公开(公告)号：WO2021211776A1

公开(公告)日：2021-10-21

申请号：PCT/US2021/027376

申请日：2021-04-15

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： ABEDI, Mohamad, H. ,  SHAPIRO, Mikhail ,  PIRANER, Dan, I. ,  LEE, Justin

IPC: C12N15/10 ,  C12N5/0783 ,  C12N15/63 ,  A61K35/17 ,  A61P35/00 ,  A61N7/02 ,  A61N2/00 ,  A61N5/02 ,  A61N5/06

Abstract: Disclosed herein include methods, compositions, and kits suitable for use in spatiotemporal regulation of therapeutic T-cells through a combination of molecular and physical actuation. There are provided, in some embodiments, thermal bioswitches that allow T- cells to sense small changes in temperature and use them as inputs for the actuation of genetic circuits. Also disclosed herein are T cell activity sensors. Genetic circuits capable of inducing expression of a payload upon thermal stimulation and/or immune cell stimulation are provided. There are provided, in some embodiments, thermally actuated immune cells and methods of using are provided. Oscillator circuits and methods of preventing T cell exhaustion are also disclosed.

公开(公告)号：EP4136228A1

公开(公告)日：2023-02-22

申请号：EP21788583.9

申请日：2021-04-15

Applicant: California Institute of Technology

Inventor： ABEDI, Mohamad, H. ,  SHAPIRO, Mikhail ,  PIRANER, Dan, I. ,  LEE, Justin

IPC: C12N15/10 ,  C12N5/0783 ,  C12N15/63 ,  A61K35/17 ,  A61P35/00 ,  A61N7/02 ,  A61N2/00 ,  A61N5/02 ,  A61N5/06