公开(公告)号：EP1805318A4

公开(公告)日：2008-08-13

申请号：EP05824763

申请日：2005-10-27

Applicant: CEPHEID

Inventor： CHANG RONALD ,  CHING JESUS ,  DORITY DOUGLAS BRYAN ,  ZHANG JIAN PING ,  WANG JAMES ,  WONG WENDY WINGKEI ,  PAUL KENDRA LARA ,  ATTA REUEL VAN

IPC: C12P19/34 ,  A21C3/04 ,  A21C11/16

CPC classification number: G01N21/6428 ,  B01L3/502 ,  B01L7/52 ,  B01L2200/0684 ,  B01L2300/0654 ,  B01L2300/0877 ,  B01L2400/0487 ,  B01L2400/0644 ,  C12Q1/6844 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q2565/629 ,  C12Q2563/173 ,  C12Q2549/119 ,  C12Q2531/143 ,  C12Q2537/149

Abstract: The invention is directed to systems, methods and apparatus for carrying out multi-stage amplification reactions, especially under fluidly closed conditions. In one aspect, methods of the invention are carried out in a fluidly closed reaction system that permits the isolation of a portion of a first (or prior) reaction mixture and its use a s a sample or specimen in a second (or subsequent) reaction mixture, thereby substantially avoiding interfering effects that first reaction components may have in the second reaction if both reaction mixture were simply combined together. In the aspect, systems, methods, and apparatus of the invention may be used with any amplification reaction that permits multiple stages of amplification based on the use of nested primers.

公开(公告)号：EP2852686A4

公开(公告)日：2016-01-06

申请号：EP13793093

申请日：2013-05-22

Applicant: CEPHEID

Inventor： WEIR ALFHAUS ,  PERSING DAVID ,  HIGUCHI RUSSELL ,  WANG JAMES ,  VANATTA REUEL ,  MURRAY WILLIAM E

IPC: C12Q1/68 ,  C12N15/11 ,  G01N33/48

CPC classification number: C12Q1/689 ,  C12N15/111 ,  C12N2310/141 ,  C12N2320/10 ,  C12Q1/6883 ,  C12Q2600/112 ,  C12Q2600/156

公开(公告)号：AU2014400858A1

公开(公告)日：2017-01-12

申请号：AU2014400858

申请日：2014-07-15

Applicant: CEPHEID

Inventor： WANG JAMES ,  ALUGUPALLY SUDHIR ,  YU ROSA ,  YOUSIF SALLY

IPC: C12Q1/68

Abstract: Compositions and methods for detecting Trichomonas vaginalis are provided.

公开(公告)号：AU2014400858B2

公开(公告)日：2021-09-16

申请号：AU2014400858

申请日：2014-07-15

Applicant: CEPHEID

Inventor： WANG JAMES ,  ALUGUPALLY SUDHIR ,  YU ROSA ,  YOUSIF SALLY

IPC: C12Q1/68

Abstract: Compositions and methods for detecting Trichomonas vaginalis are provided.

公开(公告)号：AU2013266312A1

公开(公告)日：2014-12-04

申请号：AU2013266312

申请日：2013-05-22

Applicant: CEPHEID

Inventor： WEIR ALFHOUS ,  PERSING DAVID ,  HIGUCHI RUSSELL ,  WANG JAMES ,  VANATTA REUEL ,  MURRAY WILLIAM E

IPC: C12Q1/68 ,  C12N15/11 ,  G01N33/48

Abstract: Compositions and methods for detecting Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) are provided. The present invention also provides methods and compositions for screening for infection/inflammation based on genomic copy number. Described herein is a method that entails assaying a sample obtained from the urogenital tract of the mammal for an indicator of genomic copy number, wherein a genomic copy number level that is higher than a control genomic copy number level is indicative of the presence of infection or inflammation of the urogenital tract. Also described in a kit of the invention that includes a primer and/or probe for detecting or sequencing an indicator of genomic copy number, wherein the indicator of genomic copy number comprises a nucleic acid sequence that is expected to be present in the genome of the mammal in one or two copies; and a primer and/or probe for detecting or sequencing a nucleic acid sequence that is indicative of a pathogen that infects the urogenital tract or a miRNA correlated with inflammation.

公开(公告)号：CA2953006A1

公开(公告)日：2016-01-21

申请号：CA2953006

申请日：2014-07-15

Applicant: CEPHEID

Inventor： WANG JAMES ,  ALUGUPALLY SUDHIR ,  YU ROSA ,  YOUSIF SALLY

IPC: C12Q1/68

Abstract: Compositions and methods for detecting Trichomonas vaginalis are provided.

公开(公告)号：CA2874499A1

公开(公告)日：2013-11-28

申请号：CA2874499

申请日：2013-05-22

Applicant: CEPHEID

Inventor： WEIR ALFHOUS ,  PERSING DAVID ,  HIGUCHI RUSSELL ,  WANG JAMES ,  VANATTA REUEL ,  MURRAY WILLIAM E

IPC: C12Q1/68 ,  C12N15/11 ,  G01N33/48

Abstract: Compositions and methods for detecting Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) are provided. The present invention also provides methods and compositions for screening for infection/inflammation based on genomic copy number. Described herein is a method that entails assaying a sample obtained from the urogenital tract of the mammal for an indicator of genomic copy number, wherein a genomic copy number level that is higher than a control genomic copy number level is indicative of the presence of infection or inflammation of the urogenital tract. Also described in a kit of the invention that includes a primer and/or probe for detecting or sequencing an indicator of genomic copy number, wherein the indicator of genomic copy number comprises a nucleic acid sequence that is expected to be present in the genome of the mammal in one or two copies; and a primer and/or probe for detecting or sequencing a nucleic acid sequence that is indicative of a pathogen that infects the urogenital tract or a miRNA correlated with inflammation.