公开(公告)号：JP2014064591A

公开(公告)日：2014-04-17

申请号：JP2014002146

申请日：2014-01-09

Applicant: Cepheid ,  セフィードCepheid

Inventor： CHANG RONALD ,  CHING JESUS ,  DORITY DOUGLAS BRYAN ,  ZHANG JIAN PING ,  WANG JAMES JIAN QUAN ,  WONG WENDY WINGKEI ,  PAUL KENDRA LARA ,  ATTA REUEL VAN

IPC: C12N15/09 ,  C12M1/00 ,  C12Q1/68

CPC classification number: G01N21/6428 ,  B01L3/502 ,  B01L7/52 ,  B01L2200/0684 ,  B01L2300/0654 ,  B01L2300/0877 ,  B01L2400/0487 ,  B01L2400/0644 ,  C12Q1/6844 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q2565/629 ,  C12Q2563/173 ,  C12Q2549/119 ,  C12Q2531/143 ,  C12Q2537/149

Abstract: PROBLEM TO BE SOLVED: To provide systems, methods, and an apparatus for closed multi-stage nucleic acid amplification reactions wherein a portion of a prior-stage reaction mixture serves as the sample for the next stage reaction.SOLUTION: The invention is directed to systems, methods and an apparatus for carrying out multi-stage amplification reactions, especially under fluidly closed conditions. In one aspect, the methods of the invention are carried out in a fluidly closed reaction system that permits the isolation of a portion of a first (or prior) reaction mixture and its use as a sample or specimen in a second (or subsequent) reaction mixture, thereby substantially avoiding interfering effects that first reaction components may have in the second reaction if both reaction mixtures were simply combined together. In the aspect, the systems, methods, and apparatus of the invention may be used with any amplification reaction that permits multiple stages of amplification based on the use of nested primers.

Abstract translation: 要解决的问题：提供用于闭合多级核酸扩增反应的系统，方法和装置，其中一部分前期反应混合物用作下一阶段反应的样品。解决方案：本发明涉及 系统，方法和用于进行多级扩增反应的装置，特别是在流体封闭的条件下。 在一个方面，本发明的方法在流体封闭的反应体系中进行，其允许分离第一（或先前）反应混合物的一部分，并且其在第二次（或随后的）反应中作为样品或样品的用途 从而基本上避免了如果两个反应混合物简单地组合在一起，第一反应组分可能在第二反应中可能具有干扰效应。 在这方面，本发明的系统，方法和装置可以与基于嵌套引物的使用允许多个扩增阶段的任何扩增反应一起使用。

公开(公告)号：JP2009236933A

公开(公告)日：2009-10-15

申请号：JP2009170254

申请日：2009-07-21

Applicant: Cepheid ,  セフィードCepheid

Inventor： POURAHMADI FARZAD ,  MCMILLAN WILLIAM A ,  CHING JESUS ,  CHANG RONALD ,  CHRISTEL LEE A ,  KOVACS GREGORY T ,  NORTHRUP M ALLEN ,  PETERSEN KURT E

IPC: G01N31/20 ,  G01N35/08 ,  B01D11/00 ,  B01J19/00 ,  B01L3/00 ,  B01L7/00 ,  C12M1/00 ,  C12M1/12 ,  C12M1/26 ,  C12M1/33 ,  C12M1/34 ,  C12Q1/24 ,  C40B60/14 ,  G01N1/28 ,  G01N30/60 ,  G01N33/50 ,  G01N37/00

CPC classification number: G01N30/6095 ,  B01J2219/00432 ,  B01J2219/00529 ,  B01L3/502715 ,  B01L3/502753 ,  B01L7/52 ,  B01L2200/0621 ,  B01L2200/0631 ,  B01L2200/0636 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/1827 ,  B01L2400/0421 ,  B01L2400/046 ,  B01L2400/0487 ,  B01L2400/0622 ,  B01L2400/0644 ,  B01L2400/086 ,  C12M47/02 ,  C12M47/06 ,  C40B60/14 ,  G01N30/6091 ,  Y10T436/25375 ,  Y10T436/255

Abstract: PROBLEM TO BE SOLVED: To provide a fluid manipulation cartridge that permits the rapid processing of a fluid sample that is larger in volume than any interactive region within the cartridge, allowing increased sensitivity in the detection of low copy concentrations of analytes. SOLUTION: A cartridge 101 includes a sample port 103 and a sample flow path. The sample flow path includes a component, e.g., filter paper or a microfabricated chip, for capturing the desired analyte from the sample. The cartridge 101 also includes an elution flow path to release captured analyte from the component into the elution fluid. COPYRIGHT: (C)2010,JPO&INPIT

Abstract translation: 要解决的问题：为了提供允许快速处理体积大于盒内的任何交互区域的流体样品的流体处理盒，允许在检测低拷贝浓度的分析物时提高灵敏度。 解决方案：盒101包括样品端口103和样品流动路径。 样品流动路径包括用于从样品捕获所需分析物的组分，例如滤纸或微制造芯片。 药筒101还包括洗脱流动路径，以将捕获的被分析物从组分释放到洗脱流体中。 版权所有（C）2010，JPO＆INPIT

公开(公告)号：JP2012055320A

公开(公告)日：2012-03-22

申请号：JP2011274877

申请日：2011-12-15

Applicant: Cepheid ,  セフィードCepheid

Inventor： CHANG RONALD ,  CHING JESUS ,  DORITY DOUGLAS BRYAN ,  ZHANG JIAN PING ,  WANG JAMES JIAN QUAN ,  WONG WENDY WINGKEI ,  PAUL KENDRA LARA ,  ATTA REUEL VAN

IPC: C12Q1/68 ,  C12M1/00

CPC classification number: G01N21/6428 ,  B01L3/502 ,  B01L7/52 ,  B01L2200/0684 ,  B01L2300/0654 ,  B01L2300/0877 ,  B01L2400/0487 ,  B01L2400/0644 ,  C12Q1/6844 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q2565/629 ,  C12Q2563/173 ,  C12Q2549/119 ,  C12Q2531/143 ,  C12Q2537/149

Abstract: PROBLEM TO BE SOLVED: To provide systems, methods, and apparatus for closed multistage nucleic acid amplification reactions wherein a part of a prior-stage reaction mixture serves as the sample for the next stage reaction.SOLUTION: There are provided systems, methods, and apparatus for carrying out multistage amplification reactions, especially under fluidly closed conditions. In one embodiment are carried out in a fluidly closed reaction system that permits the isolation of a part of a first (or prior) reaction mixture and its use as a sample or specimen in a second (or subsequent) reaction mixture, thereby substantially avoiding interfering effects that first reaction components may have in the second reaction if both reaction mixtures were simply combined together. In the above embodiment, the systems, the methods, and the apparatus may be used with any amplification reaction that permits multiple stages of amplification based on the use of nested primers.

Abstract translation: 要解决的问题：提供封闭多级核酸扩增反应的系统，方法和装置，其中一部分前期反应混合物用作下一阶段反应的样品。 解决方案：提供用于进行多级扩增反应的系统，方法和装置，特别是在流体关闭的条件下。 在一个实施方案中，在流体封闭的反应体系中进行，其允许在第二（或后续）反应混合物中分离第一（或在先）反应混合物的一部分及其作为样品或样品的用途，从而基本避免干扰 如果两个反应混合物简单地组合在一起，第一反应组分可能在第二反应中具有影响。 在上述实施方案中，系统，方法和装置可以与允许基于嵌套引物的使用允许多个扩增阶段的任何扩增反应一起使用。 版权所有（C）2012，JPO＆INPIT

公开(公告)号：JP2009014736A

公开(公告)日：2009-01-22

申请号：JP2008210329

申请日：2008-08-19

Applicant: Cepheid ,  シーフィード

Inventor： POURAHMADI FARZAD ,  MCMILLAN WILLIAM A ,  CHING JESUS ,  CHANG RONALD ,  CHRISTEL LEE A ,  KOVACS GREGORY T ,  NORTHRUP M ALLEN ,  PETERSEN KURT E

IPC: G01N31/20 ,  G01N35/08 ,  B01D11/00 ,  B01J19/00 ,  B01L3/00 ,  B01L7/00 ,  C12M1/00 ,  C12M1/26 ,  C12M1/33 ,  C12M1/34 ,  C40B60/14 ,  G01N1/28 ,  G01N30/60 ,  G01N33/50 ,  G01N37/00

CPC classification number: G01N30/6095 ,  B01J2219/00432 ,  B01J2219/00529 ,  B01L3/502715 ,  B01L3/502753 ,  B01L7/52 ,  B01L2200/0621 ,  B01L2200/0631 ,  B01L2200/0636 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/1827 ,  B01L2400/0421 ,  B01L2400/046 ,  B01L2400/0487 ,  B01L2400/0622 ,  B01L2400/0644 ,  B01L2400/086 ,  C12M47/02 ,  C12M47/06 ,  C40B60/14 ,  G01N30/6091 ,  Y10T436/25375 ,  Y10T436/255

Abstract: PROBLEM TO BE SOLVED: To provide a fluid manipulation cartridge that permits the rapid processing of a fluid sample that is larger in volume, allowing increased sensitivity in the detection of low copy concentrations of analytes. SOLUTION: A cartridge 101 includes a sample port 103 and a sample flow path. The sample flow path includes a component such as filter paper or a microfabricated chip for capturing the desired analyte from a sample. The cartridge 101 includes an elution flow path to release the captured analyte from the component into elution fluid. COPYRIGHT: (C)2009,JPO&INPIT

Abstract translation: 要解决的问题：为了提供允许快速处理体积较大的流体样品的流体处理盒，允许在检测低拷贝浓度的分析物时增加灵敏度。 解决方案：盒101包括样品端口103和样品流动路径。 样品流路包括诸如滤纸的组分或用于从样品捕获所需分析物的微制造芯片。 盒101包括将捕获的分析物从组分释放到洗脱流体中的洗脱流动路径。 版权所有（C）2009，JPO＆INPIT

公开(公告)号：WO0073412A3

公开(公告)日：2002-03-21

申请号：PCT/US0014738

申请日：2000-05-30

Applicant: CEPHEID

Inventor： PETERSEN KURT E ,  TAYLOR MICHAEL T ,  POURAHMADI FARZAD ,  MCMILLAN WILLIAM A ,  CHANG RONALD ,  SAKAI STANLEY H ,  CHING JESUS ,  BELGRADER PHILLIP ,  NORTHRUP M ALLEN ,  DORITY DOUGLAS B

IPC: C12M1/33 ,  B01L3/00 ,  B01L7/00 ,  C12M1/26 ,  C12N1/00 ,  G01N1/04 ,  G01N1/10 ,  G01N33/48 ,  C12M3/08

CPC classification number: B01L3/502 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0809 ,  B01L2300/087 ,  B01L2400/0487 ,  B01L2400/0622 ,  B01L2400/0644 ,  C12N1/066 ,  C12N13/00 ,  Y10S435/81 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S436/807 ,  Y10S436/809 ,  Y10S436/829 ,  Y10T137/87716 ,  Y10T137/88046 ,  Y10T436/255

Abstract: A cartridge for separating a desired analyte from a fluid sample has a sample flow path and a lysing chamber (86) in the sample flow path. The lysing chamber contains at least one filter for capturing cells or viruses from the sample as the sample flows through the lysing chamber. Beads are also disposed in the lysing chamber for rupturing the cells or viruses to release the analyte therefrom. An analyte flow path extends from the lysing chamber and diverges from the sample flow path. The analyte flow path preferably leads to a reaction chamber for chemically reacting and optically detecting the analyte. The cartridge also includes at least one flow controller (e.g., valves) for directing the sample into the waste chamber after the sample flows through the lysing chamber and for directing the analyte separated from the sample into the analyte flow path.

Abstract translation: 用于从流体样品分离所需分析物的筒在样品流动路径中具有样品流动路径和裂解室（86）。 裂解室包含至少一个用于在样品流过裂解室时从样品捕获细胞或病毒的过滤器。 珠也设置在裂解室中，用于破裂细胞或病毒以从其中释放分析物。 分析物流动路径从裂解室延伸并从样品流动路径发散。 分析物流动路径优选地导致用于化学反应和光学检测分析物的反应室。 该盒还包括至少一个流量控制器（例如阀），用于在样品流过裂解室之后将样品引入废物室，并将从样品分离的分析物引导到分析物流动路径中。

公开(公告)号：WO0073413A9

公开(公告)日：2002-01-24

申请号：PCT/US0014740

申请日：2000-05-30

Applicant: CEPHEID

Inventor： TAYLOR MICHAEL T ,  BELGRADER PHILLIP ,  POURAHMADI FRAZAD ,  MCMILLAN WILLIAM A ,  CHANG RONALD ,  SAKAI STANLEY H ,  CHING JESUS ,  PETERSEN KURT E ,  NORTHRUP M ALLEN

IPC: C12M1/33 ,  B01L3/00 ,  B01L7/00 ,  C12M1/26 ,  C12N1/00 ,  G01N1/04 ,  G01N1/10 ,  G01N33/48 ,  C12M3/08

CPC classification number: B01L3/502 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0809 ,  B01L2300/087 ,  B01L2400/0487 ,  B01L2400/0622 ,  B01L2400/0644 ,  C12N1/066 ,  C12N13/00 ,  Y10S435/81 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S436/807 ,  Y10S436/809 ,  Y10S436/829 ,  Y10T137/87716 ,  Y10T137/88046 ,  Y10T436/255

Abstract: An apparatus for disrupting cells or viruses comprises a container having a chamber for holding the cells or viruses. The container includes at least one flexible wall defining the chamber. The apparatus also includes a transducer for impacting an external surface of the flexible wall to generate pressure waves in the chamber. The apparatus also includes a pressure source for increasing the pressure in the chamber. The pressurization of the chamber ensures effective coupling between the transducer and the flexible wall. The apparatus may also include beads in the chamber for rupturing the cells or viruses.

Abstract translation: 用于破坏细胞或病毒的装置包括具有用于保持细胞或病毒的室的容器。 容器包括限定腔室的至少一个柔性壁。 该装置还包括用于冲击柔性壁的外表面的换能器，以在腔室中产生压力波。 该装置还包括用于增加腔室中的压力的​​压力源。 室的加压确保换能器与柔性壁之间的有效耦合。 该装置还可以包括室中的珠子，用于破裂细胞或病毒。

公开(公告)号：EP1805318A4

公开(公告)日：2008-08-13

申请号：EP05824763

申请日：2005-10-27

Applicant: CEPHEID

Inventor： CHANG RONALD ,  CHING JESUS ,  DORITY DOUGLAS BRYAN ,  ZHANG JIAN PING ,  WANG JAMES ,  WONG WENDY WINGKEI ,  PAUL KENDRA LARA ,  ATTA REUEL VAN

IPC: C12P19/34 ,  A21C3/04 ,  A21C11/16

CPC classification number: G01N21/6428 ,  B01L3/502 ,  B01L7/52 ,  B01L2200/0684 ,  B01L2300/0654 ,  B01L2300/0877 ,  B01L2400/0487 ,  B01L2400/0644 ,  C12Q1/6844 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q2565/629 ,  C12Q2563/173 ,  C12Q2549/119 ,  C12Q2531/143 ,  C12Q2537/149

Abstract: The invention is directed to systems, methods and apparatus for carrying out multi-stage amplification reactions, especially under fluidly closed conditions. In one aspect, methods of the invention are carried out in a fluidly closed reaction system that permits the isolation of a portion of a first (or prior) reaction mixture and its use a s a sample or specimen in a second (or subsequent) reaction mixture, thereby substantially avoiding interfering effects that first reaction components may have in the second reaction if both reaction mixture were simply combined together. In the aspect, systems, methods, and apparatus of the invention may be used with any amplification reaction that permits multiple stages of amplification based on the use of nested primers.

公开(公告)号：WO2006047777A9

公开(公告)日：2006-06-29

申请号：PCT/US2005039300

申请日：2005-10-27

Applicant: CEPHEID ,  CHANG RONALD ,  CHING JESUS ,  DORITY DOUGLAS BRYAN ,  ZHANG JIAN PING ,  WANG JAMES JIAN QUAN ,  WONG WENDY WINGKEI ,  PAUL KENDRA LARA ,  ATTA REUEL VAN

Inventor： CHANG RONALD ,  CHING JESUS ,  DORITY DOUGLAS BRYAN ,  ZHANG JIAN PING ,  WANG JAMES JIAN QUAN ,  WONG WENDY WINGKEI ,  PAUL KENDRA LARA ,  ATTA REUEL VAN

IPC: C40B40/08

CPC classification number: G01N21/6428 ,  B01L3/502 ,  B01L7/52 ,  B01L2200/0684 ,  B01L2300/0654 ,  B01L2300/0877 ,  B01L2400/0487 ,  B01L2400/0644 ,  C12Q1/6844 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q2565/629 ,  C12Q2563/173 ,  C12Q2549/119 ,  C12Q2531/143 ,  C12Q2537/149

Abstract: The invention is directed to systems, methods and apparatus for carrying out multi-stage amplification reactions, especially under fluidly closed conditions. In one aspect, methods of the invention are carried out in a fluidly closed reaction system that permits the isolation of a portion of a first (or prior) reaction mixture and its use a s a sample or specimen in a second (or subsequent) reaction mixture, thereby substantially avoiding interfering effects that first reaction components may have in the second reaction if both reaction mixture were simply combined together. In the aspect, systems, methods, and apparatus of the invention may be used with any amplification reaction that permits multiple stages of amplification based on the use of nested primers.

Abstract translation: 本发明涉及用于进行多阶段扩增反应的系统，方法和设备，特别是在流体封闭条件下。 一方面，本发明的方法在流体密闭的反应系统中进行，该反应系统允许分离第一（或之前）反应混合物的一部分并将其用作第二（或后续）反应混合物中的样品或样品， 从而基本上避免了如果两种反应混合物简单地结合在一起时第一反应组分可能在第二反应中具有的干扰作用。 在该方面，本发明的系统，方法和装置可以与允许基于使用嵌套引物的多个扩增阶段的任何扩增反应一起使用。

公开(公告)号：WO0073413A2

公开(公告)日：2000-12-07

申请号：PCT/US0014740

申请日：2000-05-30

Applicant: CEPHEID

Inventor： TAYLOR MICHAEL T ,  BELGRADER PHILLIP ,  POURAHMADI FRAZAD ,  MCMILLAN WILLIAM A ,  CHANG RONALD ,  SAKAI STANLEY H ,  CHING JESUS ,  PETERSEN KURT E ,  NORTHRUP M ALLEN

IPC: C12M1/33 ,  B01L3/00 ,  B01L7/00 ,  C12M1/26 ,  C12N1/00 ,  G01N1/04 ,  G01N1/10 ,  G01N33/48 ,  C12M3/08

CPC classification number: B01L3/502 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0809 ,  B01L2300/087 ,  B01L2400/0487 ,  B01L2400/0622 ,  B01L2400/0644 ,  C12N1/066 ,  C12N13/00 ,  Y10S435/81 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S436/807 ,  Y10S436/809 ,  Y10S436/829 ,  Y10T137/87716 ,  Y10T137/88046 ,  Y10T436/255

Abstract: An apparatus for disrupting cells or viruses comprises a container having a chamber for holding the cells or viruses. The container includes at least one flexible wall defining the chamber. The apparatus also includes a transducer for impacting an external surface of the flexible wall to generate pressure waves in the chamber. The apparatus also includes a pressure source for increasing the pressure in the chamber. The pressurization of the chamber ensures effective coupling between the transducer and the flexible wall. The apparatus may also include beads in the chamber for rupturing the cells or viruses.

Abstract translation: 用于破坏细胞或病毒的装置包括具有用于保持细胞或病毒的室的容器。 容器包括限定腔室的至少一个柔性壁。 该装置还包括用于冲击柔性壁的外表面的换能器，以在腔室中产生压力波。 该装置还包括用于增加腔室中的压力的​​压力源。 室的加压确保换能器与柔性壁之间的有效耦合。 该装置还可以包括室中的珠子，用于破裂细胞或病毒。

公开(公告)号：ES2502815T3

公开(公告)日：2014-10-06

申请号：ES05824763

申请日：2005-10-27

Applicant: CEPHEID

Inventor： CHANG RONALD ,  CHING JESUS ,  DORITY DOUGLAS BRYAN ,  ZHANG JIAN PING ,  WANG JAMES JIAN QUAN ,  WONG WENDY WINGKEI ,  PAUL KENDRA LARA ,  ATTA REUEL VAN

IPC: C12Q1/68

Abstract: Un método para realizar una reacción de amplificación anidada, comprendiendo el método las etapas de: a) realizar una reacción de amplificación de primer estadio en una cámara de reacción de un sistema cerrado de forma fluida, comprendiendo la reacción de amplificación de primer estadio uno o más polinucleótidos diana de una muestra usando reactivos de amplificación de primer estadio en una primera mezcla de reacción para formar uno o más primeros amplicones, incluyendo los reactivos de amplificación de primer estadio cebadores iniciales para cada polinucleótido diana, en donde el o los primeros amplicones se amplifican en presencia de un indicador fluorescente capaz de generar una señal óptica relacionada con una cantidad de un amplicón en la reacción de amplificación de primer estadio; b) controlar la señal óptica del indicador fluorescente en la reacción de amplificación de primer estadio en la cámara de reacción; c) retener automáticamente una parte efectiva de la mezcla de reacción de la reacción de amplificación de primer estadio cuando la señal óptica alcanza o supera un nivel umbral; d) amplificar en la cámara de reacción el o los primeros amplicones en la parte efectiva usando reactivos de amplificación de segundo estadio en una segunda mezcla de reacción para formar uno o más segundos amplicones, incluyendo los reactivos de amplificación de segundo estadio al menos un cebador secundario para cada uno del o los primeros amplicones, de modo que cada cebador secundario esté anidado en dicho primer amplicón en relación con un cebador inicial de dicho primer amplicón; y e) detectar el o los segundos amplicones para determinar la presencia o ausencia del o los polinucleótidos diana en la muestra.