公开(公告)号：CA2519830A1

公开(公告)日：2004-11-04

申请号：CA2519830

申请日：2004-03-22

Applicant: CORNELL RES FOUNDATION INC

Inventor： KMETKO JAN ,  O'NEILL KEVIN ,  THORNE ROBERT E ,  STUM ZACHARY

IPC: G01N23/20

Abstract: Sample mounts (10) for mounting microcrystals of biological macromolecules f or X-ray crystallography are prepared by using patterned thin polyimide films (12) that have curvature imparted thereto, for example, by being attached to a curved outer surface of a small metal rod (16). The patterned film (12) preferably includes a tapered tip end (24) for holding a crystal. Preferably , a small sample aperture is disposed in the film for reception of the crystal . A second, larger aperture can also be provided that is connected to the samp le aperture by a drainage channel, allowing removal of excess liquid and easier manipulation in viscous solutions. The curvature imparted to the film (12) increases the film's rigidity and allows a convenient scoop-like action for retrieving crystals. The polyimide contributes minimally to background and absorption, and can be treated to obtain desired hydrophobicity or hydrophilicity.

公开(公告)号：AU2004233143A1

公开(公告)日：2004-11-04

申请号：AU2004233143

申请日：2004-03-22

Applicant: CORNELL RES FOUNDATION INC

Inventor： THORNE ROBERT E ,  KMETKO JAN ,  O'NEILL KEVIN ,  STUM ZACHARY

IPC: G01N23/20

公开(公告)号：WO2004094993A2

公开(公告)日：2004-11-04

申请号：PCT/US2004006088

申请日：2004-03-22

Applicant: CORNELL RES FOUNDATION INC ,  THORNE ROBERT E ,  STUM ZACHARY ,  O'NEILL KEVIN ,  KMETKO JAN

Inventor： THORNE ROBERT E ,  STUM ZACHARY ,  O'NEILL KEVIN ,  KMETKO JAN

IPC: G01N23/20 ,  G01N

CPC classification number: C30B29/58 ,  C30B35/00 ,  G01N23/20 ,  G01N23/20025 ,  Y10S269/90 ,  Y10S269/908 ,  Y10S269/909 ,  Y10T29/53961 ,  Y10T117/1036

Abstract: Sample mounts (10) for mounting microcrystals of biological macromolecules for X-ray crystallography are prepared by using patterned thin polyimide films (12) that have curvature imparted thereto, for example, by being attached to a curved outer surface of a small metal rod (16). The patterned film (12) preferably includes a tapered tip end (24) for holding a crystal. Preferably, a small sample aperture is disposed in the film for reception of the crystal. A second, larger aperture can also be provided that is connected to the sample aperture by a drainage channel, allowing removal of excess liquid and easier manipulation in viscous solutions. The curvature imparted to the film (12) increases the film's rigidity and allows a convenient scoop-like action for retrieving crystals. The polyimide contributes minimally to background and absorption, and can be treated to obtain desired hydrophobicity or hydrophilicity.

Abstract translation: 用于安装用于X射线晶体学的生物大分子的微晶的样品安装件（10）通过使用具有赋予曲率的图案化的聚酰亚胺薄膜（12）来制备，例如通过附接到小金属棒的弯曲外表面 16）。 图案化膜（12）优选地包括用于保持晶体的锥形尖端（24）。 优选地，在膜中设置小的样品孔以接收晶体。 还可以提供第二个较大的孔，其通过排水通道连接到样品孔，允许去除多余的液体并且更容易在粘性溶液中操作。 赋予膜（12）的曲率提高了膜的刚性，并且允许用于取出晶体的方便的勺状作用。 聚酰亚胺对背景和吸收的贡献最小，可以进行处理以获得所需的疏水性或亲水性。

公开(公告)号：EP1623202A4

公开(公告)日：2007-04-18

申请号：EP04722504

申请日：2004-03-22

Applicant: CORNELL RES FOUNDATION INC

Inventor： THORNE ROBERT E ,  STUM ZACHARY ,  O'NEILL KEVIN ,  KMETKO JAN

IPC: G01N23/00 ,  G01N23/20

CPC classification number: C30B29/58 ,  C30B35/00 ,  G01N23/20 ,  G01N23/20025 ,  Y10S269/90 ,  Y10S269/908 ,  Y10S269/909 ,  Y10T29/53961 ,  Y10T117/1036

Abstract: Sample mounts (10) for mounting microcrystals of biological macromolecules for X-ray crystallography are prepared by using patterned thin polyimide films (12) that have curvature imparted thereto, for example, by being attached to a curved outer surface of a small metal rod (16). The patterned film (12) preferably includes a tapered tip end (24) for holding a crystal. Preferably, a small sample aperture is disposed in the film for reception of the crystal. A second, larger aperture can also be provided that is connected to the sample aperture by a drainage channel, allowing removal of excess liquid and easier manipulation in viscous solutions. The curvature imparted to the film (12) increases the film's rigidity and allows a convenient scoop-like action for retrieving crystals. The polyimide contributes minimally to background and absorption, and can be treated to obtain desired hydrophobicity or hydrophilicity.