公开(公告)号：DE69733426D1

公开(公告)日：2005-07-07

申请号：DE69733426

申请日：1997-10-03

Applicant: CORNING INC

Inventor： BOOKBINDER C ,  FEWKES J ,  GRIFFIN A ,  SMITH M ,  TENNENT L

IPC: B05D5/08 ,  B01L99/00 ,  B05D3/02 ,  C07H21/00 ,  C08J7/04 ,  C08J7/06 ,  G01N33/543 ,  B08B3/00 ,  B08B7/00 ,  C11D1/83 ,  C11D1/835 ,  C11D3/30 ,  C11D3/43

Abstract: Hydrophobic polymer surfaces whose level of protein binding is less than about 50-80 ng/cm2 are achieved by: (1) applying a coating solution composed of a solvent and a non-ionic surfactant having a HLB number of less than 5 to the surface; and (2) drying the surface to remove the solvent and thereby bring the surfactant into direct contact with the hydrophobic polymer. The combination of a low HLB number and the drying step have been found to produce low binding surfaces which can withstand multiple washes with water and/or protein-containing solutions Alternatively, the low binding surfaces can be produced by applying the non-ionic surfactant to the mold surfaces which contact molten polymer and form the polymer into a desired shape, e.g., into a multi-well plate, a pipette tip, or the like. Further, the low binding surfaces may be produced by incorporating non-soluble, non-ionic surfactants having an HLB number of less than or equal to 10 into a polymer blend prior to molding the article.

公开(公告)号：DE69733426T2

公开(公告)日：2006-04-27

申请号：DE69733426

申请日：1997-10-03

Applicant: CORNING INC

Inventor： BOOKBINDER C ,  FEWKES J ,  GRIFFIN A ,  SMITH M ,  TENNENT L

IPC: B05D5/08 ,  B08B3/00 ,  B01L99/00 ,  B05D3/02 ,  B08B7/00 ,  C07H21/00 ,  C08J7/04 ,  C08J7/06 ,  C11D1/83 ,  C11D1/835 ,  C11D3/30 ,  C11D3/43 ,  G01N33/543

Abstract: Hydrophobic polymer surfaces whose level of protein binding is less than about 50-80 ng/cm2 are achieved by: (1) applying a coating solution composed of a solvent and a non-ionic surfactant having a HLB number of less than 5 to the surface; and (2) drying the surface to remove the solvent and thereby bring the surfactant into direct contact with the hydrophobic polymer. The combination of a low HLB number and the drying step have been found to produce low binding surfaces which can withstand multiple washes with water and/or protein-containing solutions Alternatively, the low binding surfaces can be produced by applying the non-ionic surfactant to the mold surfaces which contact molten polymer and form the polymer into a desired shape, e.g., into a multi-well plate, a pipette tip, or the like. Further, the low binding surfaces may be produced by incorporating non-soluble, non-ionic surfactants having an HLB number of less than or equal to 10 into a polymer blend prior to molding the article.