公开(公告)号：WO9007259A3

公开(公告)日：1990-11-01

申请号：PCT/US8905548

申请日：1989-12-13

Applicant: COULTER ELECTRONICS

Inventor： KORTRIGHT KENNETH H ,  COULTER WALLACE H ,  RODRIGUEZ CARLOS ,  RUSSELL THOMAS ,  PAUL RONALD

IPC: G01N33/53 ,  A61K49/00 ,  C12N5/02 ,  G01N15/00 ,  G01N15/10 ,  G01N15/12 ,  G01N33/543 ,  G01N33/553 ,  G01N33/569 ,  G01N33/577

CPC classification number: G01N15/12 ,  G01N33/56972 ,  G01N2015/008 ,  G01N2015/1062 ,  G01N2015/1081

Abstract: A method and apparatus (320, 340, 360) for automatically and rapidly retrieving, counting and/or analyzing at least one selected population of cells or formed bodies, such as a white blood cell population and at least one subset thereof of a whole blood sample (322, 342) or portion thereof. A volume of a biological medium containing the white blood cells is prepared and at least one reactant (326, 346) specific or preferential at least to some selected biological cells is introduced thereto and rapidly mixed (384) for a short period of time. The opacity and/or volume parameter of the cells can be modified and the mixture is then counted and analyzed (332, 352, 416, 422) in one or more steps to obtain the desired white blood cell population and subset analysis. The biological sample can be a whole blood sample and the reactant can include or be a lyse or a monoclonal antibody bound to microspheres, which will bind to specific ones of the cells or a combination of lyse and microspheres with antibody bound thereto. The microspheres can be magnetic and the bound cells can be magnetically removed for retrieving and analyzing the remaining blood cell population.

公开(公告)号：NO885042A

公开(公告)日：1989-01-09

申请号：NO885042

申请日：1988-11-11

Applicant: COULTER ELECTRONICS

Inventor： COULTER WALLACE H ,  HOLLINGER JOHN DAVID ,  RUSSELL THOMAS ,  RODRIGUEZ CARLOS ,  PAUL RONALD ,  KORTRIGHT KENNETH H

IPC: G01N33/53 ,  B01L99/00 ,  G01N33/50 ,  G01N33/543 ,  G01N33/553 ,  G01N33/569 ,  C12Q1/24 ,  G01N33/483

CPC classification number: G01N33/5005 ,  G01N33/56966 ,  G01N33/56972

公开(公告)号：CA1339840C

公开(公告)日：1998-04-28

申请号：CA612191

申请日：1989-09-20

Applicant: COULTER ELECTRONICS

Inventor： RUSSELL THOMAS ,  KORTRIGHT KENNETH H ,  COULTER WALLACE H ,  RODRIGUEZ CARLOS M ,  PAUL RONALD ,  HAJEK CONSTANCE MARY ,  HUDSON JAMES CAREY

IPC: G01N33/53 ,  A61K49/00 ,  C12N5/02 ,  G01N15/00 ,  G01N15/10 ,  G01N15/12 ,  G01N33/543 ,  G01N33/553 ,  G01N33/569 ,  G01N33/577 ,  G01N33/546

Abstract: A method and apparatus for automatically and rapidly, retrieving, counting and/or analyzing at least one selected population of cells or formed bodies, such as a white blood cell population and at least one subset thereof of a whole blood sample or portion thereof. A volume of a biological medium containing the white blood cells is prepared and at least one reactant specific or preferential at least to some selected biological cells is introduced thereto and rapidly mixed for a short period of time. The opacity and/or volume parameter of the cells can be modified and the mixture is then counted and analyzed in one or more steps to obtain the desired white blood cell population and subset analysis. The biological sample can be a whole blood sample and the reactant can include or be a lyse or a monoclonal antibody bound to microspheres, which will bind to specific ones of the cells or a combination of lyes and microspheres with antibody bound thereto. The microspheres can be magnetic and the bound cells can be magnetically removed for retrieving and analyzing the remaining blood cell population.

公开(公告)号：NO177809B

公开(公告)日：1995-08-14

申请号：NO885042

申请日：1988-11-11

Applicant: COULTER ELECTRONICS

Inventor： COULTER WALLACE H ,  HOLLINGER JOHN DAVID ,  RUSSELL THOMAS ,  RODRIGUEZ CARLOS ,  PAUL RONALD ,  KORTRIGHT KENNETH H

IPC: B01L99/00 ,  G01N33/50 ,  G01N33/543 ,  G01N33/53 ,  G01N33/553 ,  G01N33/569 ,  G01N33/48 ,  G01N37/00

Abstract: A method and apparatus (10, 24, 46, 76, 90, 102) for accelerating at least one definitive biological reaction including increasing the accuracy of determinations made therefrom. The reaction involves selected viable biological cells which are prepared in a small sample volume and rapidly mixed with microspheres having antibody specific at least to specific ones of the cells bound thereto. The microspheres can be magnetic and the bound cells can be magnetically removed to analyze the remaining blood cell populations. The microspheres can be introduced sequentially or simultaneously.

公开(公告)号：CA1326435C

公开(公告)日：1994-01-25

申请号：CA561092

申请日：1988-03-10

Applicant: COULTER ELECTRONICS

Inventor： COULTER WALLACE H ,  HOLLINGER JOHN D ,  RUSSELL THOMAS ,  RODRIGUEZ CARLOS ,  PAUL RONALD ,  KORTRIGHT KENNETH H

IPC: G01N33/53 ,  B01L99/00 ,  G01N33/50 ,  G01N33/543 ,  G01N33/553 ,  G01N33/569 ,  G01N33/547 ,  C12Q1/02

Abstract: A method and apparatus for accelerating at least one definitive biological reaction including increasing the accuracy of determinations made therefrom. The reaction involves selected viable biological cells which are prepared in a small sample volume and rapidly mixed with microspheres having antibody specific at least to specific ones of the cells bound thereto. The microspheres can be magnetic and the bound cells can be magnetically removed to analyze the remaining blood cell populations. The microspheres can be introduced sequentially or simultaneously.

公开(公告)号：NO174225B

公开(公告)日：1993-12-20

申请号：NO885041

申请日：1988-11-11

Applicant: COULTER ELECTRONICS

Inventor： COULTER WALLACE H ,  RUSSELL THOMAS ,  RODRIGUEZ CARLOS ,  PAUL RONALD ,  KORTRIGHT KENNETH H

IPC: C12Q1/06 ,  G01N33/00 ,  G01N33/48 ,  G01N33/543

公开(公告)号：CA1311404C

公开(公告)日：1992-12-15

申请号：CA561091

申请日：1988-03-10

Applicant: COULTER ELECTRONICS

Inventor： KORTRIGHT KENNETH H ,  COULTER WALLACE H ,  RODRIGUEZ CARLOS ,  RUSSELL THOMAS ,  PAUL RONALD

IPC: G01N15/10 ,  G01N15/12 ,  G01N15/14 ,  G01N33/49 ,  G01N33/50 ,  G01N33/53 ,  G01N33/543 ,  G01N33/553 ,  G01N33/569 ,  G01N33/577 ,  G01N35/00

Abstract: A method and apparatus for automatically and rapidly, retrieving, counting and/or analyzing at least one selected white blood cell population and/or subset thereof of a whole blood sample or portion thereof. A volume of a biological medium containing the white blood cells is prepared and at least one reactant specific or preferential at least to some selected biological cells is introduced thereto and rapidly mixed for a short period of time. The opacity and/or volume parameter of the cells can be modified and the mixture is then counted and analyzed in one or more steps to obtain the desired white blood cell population analysis. The biological sample can be a whole blood sample and the reactant can include or be a lyse or a monoclonal antibody bound to microspheres, which will bind to specific ones of the cells or a combination of lyse and microspheres with antibody bound thereto. The microspheres can be magnetic and the bound cells can be magnetically removed for retrieving and analyzing the remaining blood cell population.

公开(公告)号：AU618736B2

公开(公告)日：1992-01-09

申请号：AU1681088

申请日：1988-03-11

Applicant: COULTER ELECTRONICS

Inventor： KORTRIGHT KENNETH H ,  PAUL RONALD ,  RUSSELL THOMAS ,  RODRIGUEZ CARLOS ,  COULTER WALLACE H

IPC: G01N15/10 ,  G01N15/12 ,  G01N15/14 ,  G01N33/49 ,  G01N33/50 ,  G01N33/53 ,  G01N33/543 ,  G01N33/553 ,  G01N33/569 ,  G01N33/577 ,  G01N35/00 ,  C12Q1/06 ,  G01N33/80

Abstract: A method and apparatus for automatically and rapidly, retrieving, counting and/or analyzing at least one selected white blood cell population and/or subset thereof of a whole blood sample (60) or portion thereof. A volume of a biological medium containing the white blood cells is prepared and at least one reactant (66, 74, 144, 132) specific or preferential at least to some selected biological cells is introduced thereto and rapidly mixed for a short period of time. The opacity and/or volume parameter of the cells can be modified and the mixture is then counted and analyzed in one or more steps to obtain the desired white blood cell population analysis. The biological sample can be a whole blood sample and the reactant can include or be a lyse (66) or a monoclonal antibody bound to microspheres (132, 144), which will bind to specific ones of the cells or a combination of lyse and microspheres with antibody bound thereto. The microspheres can be magnetic and the bound cells can be magnetically removed for retrieving and analyzing the remaining blood cell population.

公开(公告)号：AU4821890A

公开(公告)日：1990-08-01

申请号：AU4821890

申请日：1989-12-13

Applicant: COULTER ELECTRONICS

Inventor： KORTRIGHT KENNETH H ,  COULTER WALLACE H ,  RODRIGUEZ CARLOS ,  RUSSELL THOMAS ,  PAUL RONALD

IPC: G01N33/53 ,  A61K49/00 ,  C12N5/02 ,  G01N15/00 ,  G01N15/10 ,  G01N15/12 ,  G01N33/543 ,  G01N33/553 ,  G01N33/569 ,  G01N33/577 ,  G01N33/49

公开(公告)号：ES2011652A6

公开(公告)日：1990-02-01

申请号：ES8800746

申请日：1988-03-11

Applicant: COULTER ELECTRONICS

Inventor： KORTRIGHT KENNETH H ,  COULTER WALLACE H ,  RODRIGUEZ CARLOS ,  RUSSELL THOMAS ,  PAUL RONALD

IPC: G01N15/10 ,  G01N15/12 ,  G01N15/14 ,  G01N33/49 ,  G01N33/50 ,  G01N33/53 ,  G01N33/543 ,  G01N33/553 ,  G01N33/569 ,  G01N33/577 ,  G01N35/00 ,  G01N27/26 ,  G01N27/72

Abstract: ANALIZADOR AUTOMATICO Y METODO PARA SEPARA UNA POBLACION DE CELULAS DE UNA MUESTRA DE SANGRE. SE DESCRIBEN UN METODO Y UN APARATO PARA EXTRAER, CONTAR Y/O ANALIZAR, DE MANERA AUTOMATICA Y RAPIDA, POR LO MENOS, UNA POBLACION ELEGIDA DE CELULAS BLANCAS DE LA SANGRE Y/O UN SUBGRUPO DE LA MISMA, DE UNA MUESTRA DE SANGRE COMPLETA O DE UNA PARTE DE LA MISMA. SE PREPARA UN VOLUMEN DE MEDIO BIOLOGICO CONTENIENDO LAS CELULAS BLANCAS DE LA SANGRE Y SE LE A/ADE, POR LO MENOS, UN AGENTE REACTIVO ESPECIFICO PARA ALGUNAS CELULAS BIOLOGICAS ELEGIDAS, Y SE MEZCLA RAPIDAMENTE EN UN CORTO PERIODO DE TIEMPO. EL PARAMETRO DE OPACIDAD Y/O DE VOLUMEN DE LAS CELULAS PUEDE SER MODIFICADO Y A CONTINUACION SE EFECTUA EL RECUENTO Y EL ANALISIS DE LA MEZCLA EN UNA O VARIAS ETAPAS PARA OBTENER EL ANALISIS DESEADO DE LA POBLACION DE CELULAS BLANCAS DE LA SANGRE. LA MUESTRA BIOLOGICA PUEDE SER UNA MUESTRA DE SANGRE COMPLETA Y EL AGENTE REACTIVO PUEDE INCLUIR O SER UN REACTIVO LITICO O UN ANTICUERPO MONOCLONAL UNIDO A MICROESFERAS, LAS CUALES SE ADHERIRAN A CIERTAS CELULAS ESPECIFICAS, O UNA COMBINACION DE REACTIVO LITICO Y MICROESFERAS CON ANTICUERPO UNIDO A ELLAS. LAS MICROESFERAS PUEDEN SER MAGNETICAS Y LAS CELULAS UNIDAS A ELLAS PUEDEN SER ELIMINADAS MAGNETICAMENTE PARA OBTENER Y ANALIZAR LA RESTANTE POBLACION DE CELULAS BLANCAS.