公开(公告)号：WO9007259A3

公开(公告)日：1990-11-01

申请号：PCT/US8905548

申请日：1989-12-13

Applicant: COULTER ELECTRONICS

Inventor： KORTRIGHT KENNETH H ,  COULTER WALLACE H ,  RODRIGUEZ CARLOS ,  RUSSELL THOMAS ,  PAUL RONALD

IPC: G01N33/53 ,  A61K49/00 ,  C12N5/02 ,  G01N15/00 ,  G01N15/10 ,  G01N15/12 ,  G01N33/543 ,  G01N33/553 ,  G01N33/569 ,  G01N33/577

CPC classification number: G01N15/12 ,  G01N33/56972 ,  G01N2015/008 ,  G01N2015/1062 ,  G01N2015/1081

Abstract: A method and apparatus (320, 340, 360) for automatically and rapidly retrieving, counting and/or analyzing at least one selected population of cells or formed bodies, such as a white blood cell population and at least one subset thereof of a whole blood sample (322, 342) or portion thereof. A volume of a biological medium containing the white blood cells is prepared and at least one reactant (326, 346) specific or preferential at least to some selected biological cells is introduced thereto and rapidly mixed (384) for a short period of time. The opacity and/or volume parameter of the cells can be modified and the mixture is then counted and analyzed (332, 352, 416, 422) in one or more steps to obtain the desired white blood cell population and subset analysis. The biological sample can be a whole blood sample and the reactant can include or be a lyse or a monoclonal antibody bound to microspheres, which will bind to specific ones of the cells or a combination of lyse and microspheres with antibody bound thereto. The microspheres can be magnetic and the bound cells can be magnetically removed for retrieving and analyzing the remaining blood cell population.

公开(公告)号：US3603875A

公开(公告)日：1971-09-07

申请号：US3603875D

申请日：1969-05-12

Applicant: COULTER ELECTRONICS

Inventor： COULTER WALLACE H ,  HOGG WALTER R

IPC: G01N15/12 ,  G01N33/49 ,  G01N27/00

CPC classification number: G01N15/1245

Abstract: Disclosed is an improved method and apparatus for analyzing particles by the Coulter principle of employing an impedance responsive detecting aperture through which pass particles in suspension. Employed herein are a plurality of logically parallel detecting apertures, preferably of different microscopic sizes, each aperture feeding circuitry which is subdivided into a plurality of channels, each responsive to a different narrow subrange of particle size. By time and volume related elements, there is generated an output voltage which is proportional to particle volume per unit time over the entire particle system; hence, statistically valid data is available at all times during an analysis run, even in the event of a malfunctioning blockage of an aperture.

公开(公告)号：US3502973A

公开(公告)日：1970-03-24

申请号：US3502973D

申请日：1968-03-18

Applicant: COULTER ELECTRONICS

Inventor： COULTER WALLACE H ,  HOGG WALTER R

IPC: G01N15/12 ,  G01R27/02 ,  H03K5/26 ,  G06F7/38 ,  G01N27/00

CPC classification number: H03K5/26 ,  G01N15/12 ,  G01N15/1227 ,  G01R27/02

公开(公告)号：US3392331A

公开(公告)日：1968-07-09

申请号：US47989165

申请日：1965-08-16

Applicant: COULTER ELECTRONICS

Inventor： COULTER WALLACE H

IPC: G01N15/12

CPC classification number: G01N15/12

公开(公告)号：US3259891A

公开(公告)日：1966-07-05

申请号：US36407064

申请日：1964-05-01

Applicant: COULTER ELECTRONICS

Inventor： COULTER WALLACE H ,  HOGG WALTER R

IPC: G01N15/12

CPC classification number: G01N15/12 ,  Y10T137/4259

公开(公告)号：US3138290A

公开(公告)日：1964-06-23

申请号：US22061662

申请日：1962-08-31

Applicant: COULTER ELECTRONICS

Inventor： COULTER WALLACE H

IPC: B01J4/00 ,  G01N1/38 ,  G05D11/00

CPC classification number: G01N1/38 ,  B01J4/008 ,  G05D11/006

公开(公告)号：US3557352A

公开(公告)日：1971-01-19

申请号：US3557352D

申请日：1967-11-01

Applicant: COULTER ELECTRONICS

Inventor： HOGG WALTER R ,  COULTER WALLACE H

IPC: G01D1/14 ,  G01N15/12 ,  G06M11/00 ,  G01N15/02

CPC classification number: G01N15/1227

Abstract: Apparatus which uses the Coulter particle analyzing system, converting the pulses obtained from said system into accumulated electrical quantities which may be compared to one another or to other quantities derived from the same system on the basis of which one can adjust the quantities relative to one another to achieve the size of a particle which divides the system into two fractional parts having a particular relationship to one another. A variation of this structure is one in which a particle size is obtained which establishes a point in the particulate system above or below which a predetermined fraction of the total mass exists. The preferred structure uses a threshold circuit responding to particle size and separating the electrical pulses produced by the particles being scanned in the Coulter apparatus through the use of such a threshold. The pulse trains are converted into current and these are compared, adjusting the threshold up and down until the desired relationship is reached in a comparison device. The level of the threshold being calibrated to particle size then represents the dividing size between the two fractions. One of the most useful pieces of information of this kind is the dividing size which is the mass median, in which the respective masses of particulate matter above and below the dividing size are equal to one another.

公开(公告)号：US3473010A

公开(公告)日：1969-10-14

申请号：US3473010D

申请日：1965-03-22

Applicant: COULTER ELECTRONICS

Inventor： BLOOMFIELD LEO B ,  HOGG WALTER R ,  COULTER WALLACE H ,  DORMAN ERVIN L

IPC: G01N15/12 ,  G01N33/49 ,  G06G7/48 ,  G06G7/50

CPC classification number: G01N15/1227

公开(公告)号：US3444464A

公开(公告)日：1969-05-13

申请号：US3444464D

申请日：1965-11-26

Applicant: COULTER ELECTRONICS

Inventor： COULTER WALLACE H ,  HOGG WALTER R

IPC: G01N15/12 ,  G01R19/00 ,  H03K5/24 ,  G01N27/02 ,  G01N27/06

CPC classification number: G01R19/003 ,  G01N15/1245 ,  H03K5/24 ,  Y10S65/06 ,  Y10T137/4807

公开(公告)号：US3444463A

公开(公告)日：1969-05-13

申请号：US3444463D

申请日：1966-02-14

Applicant: COULTER ELECTRONICS

Inventor： COULTER WALLACE H ,  HOGG WALTER R

IPC: G01N15/12 ,  G01R19/00 ,  H03K5/24 ,  G01N27/00 ,  G06F7/38 ,  G06G7/00

CPC classification number: G01R19/003 ,  G01N15/1245 ,  H03K5/24 ,  Y10S65/06 ,  Y10T137/4807