公开(公告)号：US09340765B2

公开(公告)日：2016-05-17

申请号：US13850988

申请日：2013-03-26

Applicant: California Institute of Technology

Inventor： Frederick Balagadde ,  Carl L. Hansen ,  Emil Kartalov ,  Stephen R. Quake

IPC: C12M1/00 ,  C12M1/18 ,  C12M1/33 ,  B01L3/00 ,  C12M3/06 ,  A01P1/00

CPC classification number: C12P19/04 ,  B01L3/5027 ,  B01L3/502738 ,  B01L2300/0861 ,  B01L2300/0877 ,  B01L2300/10 ,  B01L2300/163 ,  B01L2400/0481 ,  B01L2400/0655 ,  C12M23/16 ,  C12M23/34 ,  C12M39/00 ,  C12P1/00

Abstract: A method to suppress biofilm formation in a growth chamber of a chemostat is described. The method includes the steps of adding a lysis agent to an isolated portion of the growth chamber, and reuniting the isolated portion with the rest of the growth chamber. The microfluidic chemostat includes a growth chamber having a plurality of compartments. Each of the compartments may be fluidly isolated from the rest of the growth chamber by one or more actuatable valves. The chemostat also included a nutrient supply-line to supply growth medium to the growth chamber, and an output port to remove fluids from the growth chamber.

Abstract translation: 描述了抑制恒化器生长室中生物膜形成的方法。 该方法包括以下步骤：将裂解剂加入生长室的分离部分，并将分离部分与生长室的其余部分重新连接。 微流控恒化器包括具有多个隔室的生长室。 每个隔室可以通过一个或多个可致动阀与生长室的其余部分流体隔离。 恒化器还包括向生长室供应生长培养基的营养供应线以及从生长室中除去流体的输出端口。