DNA SEQUENCE FOR DETECTING HUMAN ALBLISM AND DETECTION OF HUMAN ALBINISM USING THE SAME

    公开(公告)号:JPH06100A

    公开(公告)日:1994-01-11

    申请号:JP18588592

    申请日:1992-06-18

    Applicant: DAICEL CHEM

    Abstract: PURPOSE:To provide a new DNA sequence useful for the diagnosing, preventing or curing human albinism. CONSTITUTION:The objective DNA sequence, in human tyrosinase gene or its complementary strand, containing a sequence made up of at least 12 (pref. >=20) consecutive bases capable of hybridization at the upstream and/or downstream side of the 310 th base from the transfer-starting site; e.g. 5'- GCAGTTTGGTCCCCAAAAGCC-3'. This DNA sequence can be synthesized by a conventional means using a DNA synthesizer and isolated and purified by HPLC.

    VACCINE FOR POLLINOSIS OF SUGI AND HINOKI

    公开(公告)号:JPH0390036A

    公开(公告)日:1991-04-16

    申请号:JP22645189

    申请日:1989-08-31

    Applicant: DAICEL CHEM

    Abstract: PURPOSE:To obtain a vaccine, containing an antigenic protein, extracted and purified from pollens of HINOKI (Chamaecyparis obtusa Endl.) as an active ingredient and useful for diagnosing, preventing and treating pollinosis of SUGI (Cryptomeria japonica D. Don) and the HINOKI. CONSTITUTION:Pollens of HINOKI are extracted using a glucose solution containing phenol added thereto, sodium hydrogencarbonate solution, physiological saline solution, etc., preferably tris(hydroxymethyl)aminomethane (tris) hydrochloric acid solution as an extracting solvent according to a normally used method and the resultant crude extract is separated by a normally used method for purifying proteins to provide an antigenic protein having antigenicity common to the pollens of SUGI and HINOKI, 40000-45000 molecular weight and 6.5-6.8 isoelectric point measured by an electrophoretic method using polyacrylamide. A vaccine containing the aforementioned protein is subcutaneously or orally administered for a prescribed period to effectively inhibit production of IgE antibodies against pollinic antigen of the SUGI and HINOKI and simultaneously promote production of the IgG antibody and IgA antibody which are blocking antibodies against the pollinic antigen penetrated into mucous membranes or bodies.

    FISH ATTRACTANT
    5.
    发明专利

    公开(公告)号:JPS61162141A

    公开(公告)日:1986-07-22

    申请号:JP145985

    申请日:1985-01-10

    Applicant: DAICEL CHEM

    Abstract: PURPOSE:To obtain an excellent fish attractant, by compounding a fish bait component with fiber powder and a carboxymethylcellulose alkali metal salt. CONSTITUTION:A fish bait component such as chrysalis powder, rice bran, etc. is compounded with powdered fiber such as natural fiber and a carboxymethylcellulose alkali metal salt. The metal salt is preferably composed of 1-99pts.wt. of carboxymethylcellulose sodium salt and 99-1pts.wt. of carboxymethylcellulose calcium salt, and is a hardly soluble mixture of sol and gel acting as a binder. The ratios of the metal salt, fiber powder and the fish bait component are selected according to the purpose. For the fish-luring purpose, the agent should be disintegrated within a proper time when thrown into water. For example, a paste bait can be produced by mixing 20-40pts.wt. of the metal salt, 25-40pts.wt. of the fiber powder and 40-60pts.wt. of the fish bait component.

    HUMAN LYMPHOCYTIC LEUKOCYTE AND IMMUNE TOXIC MATERIAL DETECTING METHOD USING IT

    公开(公告)号:JP2000121625A

    公开(公告)日:2000-04-28

    申请号:JP29205098

    申请日:1998-10-14

    Applicant: DAICEL CHEM

    Abstract: PROBLEM TO BE SOLVED: To provide a human lymphocytic leukocyte useful for detecting a material having the endocrine disturbance action or immune toxic action to the human body simply and quantitatively. SOLUTION: By using the human lymphocytic leukocyte causing apoptosis by immune toxic action, a material having the endocrine disturbance action or immune toxic action to the human body can be detected. The human lymphocytic leukocyte includes human leukocyte T cell line L-MAT cell and Jurkat cells selected by apoptosis sensitivity to the dioxin compound and human lymphoblast T cell line such as Molt-4 cell or the like. 3-(4,5-dimethylthiazole-2- il)-2,5-diphenyltetrazolium bromide can be used as detection reagent for detecting extrinsic endocrine disturbance material or immune toxic material.

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