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    SALT CONVERSION AND PURIFICATION OF OLIGOPEPTIDE BY ONE PROCESS
    1.
    发明专利
    SALT CONVERSION AND PURIFICATION OF OLIGOPEPTIDE BY ONE PROCESS 失效

    公开(公告)号：JPH11310595A

    公开(公告)日：1999-11-09

    申请号：JP8438699

    申请日：1999-03-26

    Applicant: DEGUSSA

    Inventor： GUENTHER KURT DR ,  KUNZ FRANZ-RUDOLF ,  DRAUZ KARLHEINZ ,  MUELLER THOMAS

    IPC: C07K1/20 ,  C07K1/12 ,  C07K1/36 ,  C07K7/23 ,  G01N30/46 ,  G01N30/88

    Abstract: PROBLEM TO BE SOLVED: To convert an oligopeptide to salt and to purify the salt by one process and to obtain an oligopeptide acetate by using an oligopeptide in the form of hydrochloride and subjecting the oligopeptide to liquid chromatography using an acetate-containing solvent. SOLUTION: In the method for salt conversion and purification of an oligopeptide by one process by a liquid chromatography using an acetate- containing solvent to obtain an oligopeptide acetate, the oligopeptide is used in its hydrochloride form for purification so that the oligopeptide is converted into a salt and purified by one process. Preferably a high-pressure liquid chromatography is used as the liquid chromatography, a solvent composed of a solvent A comprising 85-95% water, 2-10% acetic acid and 0-5% acetonitrile and a solvent B comprising 20-48% water, 2-10% acetic acid and 50-70% acetonitrile is used as a solvent base and the process is carried out at a temp of 5-50 deg.C under a pressure of 5-10 bar.

    IN-PROCESS CONTROL IN A METHOD FOR PRODUCING EPO
    2.
    发明专利
    IN-PROCESS CONTROL IN A METHOD FOR PRODUCING EPO 未知

    公开(公告)号：CA2747320A1

    公开(公告)日：2010-07-08

    申请号：CA2747320

    申请日：2009-12-07

    Applicant: EVONIK DEGUSSA GMBH

    Inventor： KUNZ FRANZ-RUDOLF ,  GLASER FLORIAN ,  WIENAND WOLFGANG ,  HANKO RUDOLF ,  EUL WILFRIED ,  REICHERT DIETMAR

    IPC: G01N33/74

    Abstract: The invention relates to a method for determining the isoform composition of erythropoietin, comprising the following steps: a) isoelectrical focusing of a sample comprising erythropoietin in a gel over a pH range having a lower limit of 2.5 to 3.5 and an upper limit of 5 to 8, wherein the sample comprising erythropoietin originates from a culture supernatant of erythropoietin producing eukaryotic cells; b) transferring of the proteins comprised and separated in the gel to a membrane; c) verifying the erythropoietin bound to the membrane by specific antibodies; and to a method for in-process control of culture supernatants of erythropoietin producing eukaryotic cells during the fermentative production process.

    IN-PROCESS CONTROL IN A METHOD FOR PRODUCING EPO
    6.
    发明专利
    IN-PROCESS CONTROL IN A METHOD FOR PRODUCING EPO 未知

    公开(公告)号：SG172203A1

    公开(公告)日：2011-07-28

    申请号：SG2011044179

    申请日：2009-12-07

    Applicant: EVONIK DEGUSSA GMBH

    Inventor： KUNZ FRANZ-RUDOLF ,  GLASER FLORIAN ,  WIENAND WOLFGANG ,  HANKO RUDOLF ,  EUL WILFRIED ,  REICHERT DIETMAR

    Abstract: The invention relates to a method for determining the isoform composition of erythropoietin, comprising the following steps:isoelectrical focusing of a sample comprising erythropoietin in a gel overa pH range having a lower limit of 2.5 to 3.5 and an upper limit of 5 to 8, wherein the sample comprising erythropoietin originates from a culture super natant of erythropoietin producing eukaryotic cells;transferring of the proteins comprised and separated in the gel to a mem brane;c) verifying the erythropoietin bound to the membrane by specific antibod ies;and to a method for in-process control of culture supernatants of erythropoietin producing eukaryotic cells during the fermentative production process.

    NOVEL CHIRAL SELECTORS AND STATIONARY PHASES FOR SEPARATING ENANTIOMER MIXTURES
    9.
    发明专利
    NOVEL CHIRAL SELECTORS AND STATIONARY PHASES FOR SEPARATING ENANTIOMER MIXTURES 未知

    公开(公告)号：CA2718240A1

    公开(公告)日：2009-09-17

    申请号：CA2718240

    申请日：2009-02-16

    Applicant: EVONIK DEGUSSA GMBH

    Inventor： KUNZ FRANZ-RUDOLF ,  RICHTER PETER ,  MERGET STEFAN ,  SINGER ROLAND ,  MULLER THOMAS

    IPC: B01J20/29

    Abstract: The invention relates to the use of a-unsubstituted ß-amino acid derivatives having the structure (I), as chiral selectors in methods for separating mixtures of substances, preferably of mixtures of chiral substances, more preferred of enantiomer mixtures, especially of enantiomers of substances that are selected from the group containing ß-amino acids and the derivatives thereof, a-amino acids and a-hydroxy acids, wherein n = 1-5, preferably n = 1 or n = 2, R1 = (C1-C4) alkyl, (C6-C10) aryl, (C7-C13) aralkyl, (C7-C10) heteroaralkyl, pyridyl, hydroxymethyl, CH(OH)CH3, CH2CONH2, CH2COOH, (CH2)2CONH2, (CH2)2COOH, (CH2)4NH2, (CH2)2SCH3 or (CH2)3NHC(NH)NH2 and R2 = 3,5-dinitrobenzoyl or naphthyl, if X is a linker for the covalent bond to a carrier material, or R2 = CH2CHR3R4, wherein R3 = H or OH and R4 = (C1-C20) alkyl, (C6-C10) aryl or (C7-C13) aralkyl, if X = OH, and to the use of said selectors for producing chiral stationary phases. The invention further relates to the chiral stationary phases derived from the above chiral selectors, to methods for producing the same and for the separation by chromatography of mixtures of substances, preferably of mixtures of chiral substances, more preferred of enantiomer mixtures, especially of enantiomers of substances that are selected from the group containing ß-amino acids and the derivatives thereof, a-amino acids and a-hydroxy acids.

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