公开(公告)号：JP2001190297A

公开(公告)日：2001-07-17

申请号：JP2000376438

申请日：2000-12-11

Applicant: DEGUSSA

Inventor： MOECKEL BETTINA ,  PFEFFERLE WALTER ,  MARX ACHIM

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/02 ,  C12N15/31 ,  C12N15/53 ,  C12P13/08 ,  C12P13/14 ,  C12R1/15

Abstract: PROBLEM TO BE SOLVED: To provide a method for producing an amino acid, especially L-lysine by an improved fermentation. SOLUTION: This method for isolating new genes sdhC, sdhA and sdhB from coryneform bacteria is provided, and the improved fermenting method for producing an L-amino acid by attenuating the sdhA-, sdhB- or sdhC-gene- encoding subunits A, B or C of an enzyme, succinic acid dehydrogenase is also provided.

公开(公告)号：JP2001197891A

公开(公告)日：2001-07-24

申请号：JP2000363631

申请日：2000-11-29

Applicant: DEGUSSA

Inventor： MOECKEL BETTINA ,  PFEFFERLE WALTER

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/90 ,  C12N15/31 ,  C12N15/54 ,  C12N15/61 ,  C12P13/04 ,  C12P13/08 ,  C12Q1/68 ,  C12R1/15

Abstract: PROBLEM TO BE SOLVED: To provide a new fermentative method for producing an amino acid, particularly L-lysine, by the use of a nucleotide sequence encoding gpm gene and a corynebacterium having amplified gpm gene. SOLUTION: This fermentative method is carried out by using a polynucleotide isolated from a corynebacterium containing a polynucleotide sequence selected from the following groups: a) a polynucleotide showing at least 70% of homogeneity to a polynucleotide which codes for a polypeptide containing a specific amino acid sequence derived from Corynebacterium glutamicum, b) a polynucleotide encoding a polypeptide containing an amino acid sequence which shows at least 70% of homogeneity to the amino acid sequence, c) a polynucleotide complementary to the polynucleotide a) or b) and d) a polynucleotide containing at least 15 of successive bases in the polynucleotide sequence a), b) or c).

公开(公告)号：JP2001186895A

公开(公告)日：2001-07-10

申请号：JP2000354308

申请日：2000-11-21

Applicant: DEGUSSA

Inventor： MOECKEL BETTINA ,  PFEFFERLE WALTER

IPC: C12N15/09 ,  C12N1/20 ,  C12N1/21 ,  C12N9/12 ,  C12N15/54 ,  C12P13/04 ,  C12P13/08 ,  C12Q1/68 ,  C12R1/15

Abstract: PROBLEM TO BE SOLVED: To provide a new nucleotide sequence encoding the pfk-gene. SOLUTION: The new nucleotide sequence encoding the pfk-gene has the following nucleotide sequence selected from (a), (b) and (c): (a) the polynucleotide that has at least 70% homology to the polynucleotide encoding the specific amino acid sequence originating from Corynebacterium glutamicum; (b) the polynucleotide that encodes the polypeptide including the amino acid sequence that has at least 70% homology to the above-stated amino acid sequence; (c) the polynucleotides that are complementary to the polynucleotides (a) and (b); and (d) the polynucleotides that include at least 15 bases-linking bases in the polynucleotides (a), (b) or (c); and can be isolated from the cell bodies of Corynebacterium and the pfk-gene is enhanced and L-amino acids are produced by fermentation.

公开(公告)号：JP2001161380A

公开(公告)日：2001-06-19

申请号：JP2000305110

申请日：2000-10-04

Applicant: DEGUSSA

Inventor： MOECKEL BETTINA ,  PFEFFERLE WALTER ,  THOMAS HERRMANN ,  PUEHLER ALFRED ,  KALINOWSKI JOERN ,  BATHE BRIGITTE

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/88 ,  C12N15/54 ,  C12N15/60 ,  C12P13/04 ,  C12P13/08 ,  C12R1/15

Abstract: PROBLEM TO BE SOLVED: To provide a newly improved method for producing amino acid, particularly L-lysine, by fermentation. SOLUTION: This method comprises using a nucleotide sequence encoding eno gene and a coryneform bacterium having eno genes that was amplified, particularly excessively expressed.

公开(公告)号：JP2001299372A

公开(公告)日：2001-10-30

申请号：JP2001083004

申请日：2001-03-22

Applicant: DEGUSSA

Inventor： MOECKEL BETTINA ,  WEISSENBORN ANKE ,  PFEFFERLE WALTER ,  HARTMANN MICHAEL ,  KALINOWSKI JOERN ,  PUEHLER ALFRED

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/10 ,  C12N15/31 ,  C12N15/54 ,  C12P13/08 ,  C12Q1/68 ,  C12R1/13

Abstract: PROBLEM TO BE SOLVED: To provide an improved method for producing L-lysine by fermentation. SOLUTION: This isolated polynucleotide has a polynucleotide sequence selected from the group consisting of (a) a polynucleotide at least 70% identical to a polynucleotide encoding the polypeptide having a specific amino acid sequence derived from Corynebacterium glutamicum, (b) a polynucleotide encoding a polypeptide having an amino acid sequence at least 70% identical to the amino acid sequence, (c) a polynucleotide complementary to the polynucleotide (a) or (b), and (d) a polynucleotide having 15 nucleotides or more of a sequence of the polynucleotide (a), (b), or (c).

公开(公告)号：JP2001197892A

公开(公告)日：2001-07-24

申请号：JP2000371850

申请日：2000-12-06

Applicant: DEGUSSA

Inventor： MOECKEL BETTINA ,  WEISSENBORN ANKE ,  PFEFFERLE WALTER ,  MARX ACHIM ,  PUEHLER ALFRED ,  KALINOWSKI JOERN ,  BATHE BRIGITTE ,  DUSCH NICOLE

IPC: C12N15/09 ,  C07K14/34 ,  C12N1/21 ,  C12N9/00 ,  C12N15/01 ,  C12N15/31 ,  C12N15/53 ,  C12P13/08 ,  C12R1/15

Abstract: PROBLEM TO BE SOLVED: To provide a new ameliorated method on a fermentative production for an amino acid, particularly L-lysine. SOLUTION: This method is carried out by using a polynucleotide containing a polynucleotide sequence selected from the following groups: a) a polynucleotide showing at least 70% of homogeneity to a polynucleotide which codes for a polypeptide containing a specific amino acid sequence derived from Corynebacterium glutamicum, b) a polynucleotide encoding a polypeptide containing an amino acid sequence which shows at least 70% of homogeneity to the specific amino acid sequence derived from Corynebacterium glutamicum, c) a polynucleotide complementary to the polynucleotide a) or b) and d) a polynucleotide containing at least 15 of nucleotides in the polynucleotide sequence a), b) or c).

公开(公告)号：JP2001169788A

公开(公告)日：2001-06-26

申请号：JP2000324315

申请日：2000-10-24

Applicant: DEGUSSA ,  FORSCHUNGSZENTRUM JUELICH GMBH

Inventor： KENNERKNECHT NICOLE ,  SAHM HERMANN ,  EGGELING LOTHAR ,  PFEFFERLE WALTER

IPC: C12N15/09 ,  C07K14/34 ,  C12N1/21 ,  C12N15/31 ,  C12N15/54 ,  C12P13/06 ,  C12P13/08 ,  C12R1/15

Abstract: PROBLEM TO BE SOLVED: To provide a newly improved fermentative producing method of branched amino acids. SOLUTION: The fermentative producing method of branched amino acids is carried out by using an isolated polynucleotide including at least one of polynucleotides selected from the group consisting of (a) a polynucleotide which shows at least 70% of homogeneity to the polynucleotide that codes for a polypeptide containing at least one of either of two kinds of specific amino acid sequences derived from Corynebacterium glutamicum, (b) a polynucleotide encoding a polypeptide containing an amino acid sequence which shows at least 70% of homogeneity to the specific amino acid sequence, (c) a polynucleotide complementary to the polynucleotide (a) or (b) and (d) a polynucleotide containing at least 15 successive bases in the polynucleotide sequence of (a), (b) or (c).

公开(公告)号：JP2001037495A

公开(公告)日：2001-02-13

申请号：JP2000202550

申请日：2000-07-04

Applicant: DEGUSSA ,  FORSCHUNGSZENTRUM JUELICH GMBH

Inventor： KREUTZER CAROLINE ,  MOECKEL BETTINA ,  PFEFFERLE WALTER ,  EGGELING LOTHAR ,  SAHM HERMANN ,  PATEK MIROSLAV

IPC: C12N15/09 ,  C07K14/34 ,  C12N1/21 ,  C12N15/53 ,  C12N15/54 ,  C12N15/67 ,  C12N15/77 ,  C12P13/08 ,  C12R1/15

Abstract: PROBLEM TO BE SOLVED: To provide a novel Escherichia or Corynebacterium strain that has enhanced pyc gene in which dapA-gene, lysE-gene, dapB gene and the like are together enhanced simultaneously, and is useful for production of L-lysine. SOLUTION: The objective novel L-lysine-producing Corynebacterium has enhanced pyc (pyruvic acid carboxylase) gene, additionally the genes in this bacterium, selected from the dapA (dihydrodipicolinic acid synthase) gene represented by formula I, lysC (aspartic acid kinase)-gene, lysE (lysine-export carrier)gene and dapB (dihydrodipicholinic acid reductase) gene are enhanced individually or together and is useful for production of L-lysine and the like. The bacterium is prepared by cloning the gene relating to the production of L-lysine from the chromosomal DNA of Corynebacterium glutamicum and transducing the cloned DNA into a Corybacterium.

公开(公告)号：JP2001190296A

公开(公告)日：2001-07-17

申请号：JP2000376435

申请日：2000-12-11

Applicant: DEGUSSA ,  FORSCHUNGSZENTRUM JUELICH GMBH

Inventor： ZIGLER PETRA ,  EGGELING LOTHAR ,  SAHM HERMANN ,  THIERBACH GEORG DR ,  PFEFFERLE WALTER

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/10 ,  C12N9/99 ,  C12N15/01 ,  C12N15/31 ,  C12N15/54 ,  C12P13/04 ,  C12P13/08 ,  C12R1/15 ,  C12R1/19

Abstract: PROBLEM TO BE SOLVED: To provide a fermentation method for producing an L-amino acid, especially L-threonine, by using a Corynebacterium having an attenuated glyA gene. SOLUTION: This method for producing the L-amino acid comprises conducting the following processes: (a) culturing a Corynebacterium having an at least attenuated glyA gene and producing a desired L-amino acid; (b) increasing the desired product in a culturing medium or in the cell of the bacterium; (c) isolating the L-amino acid.

公开(公告)号：JP2001186896A

公开(公告)日：2001-07-10

申请号：JP2000354681

申请日：2000-11-21

Applicant: DEGUSSA

Inventor： MOECKEL BETTINA ,  PFEFFERLE WALTER

IPC: C12N15/09 ,  C12N1/20 ,  C12N1/21 ,  C12N9/12 ,  C12N15/54 ,  C12P13/08 ,  C12R1/15

Abstract: PROBLEM TO BE SOLVED: To provide a new method for producing amino acids, particularly an improved fermentation process for producing L-lysine. SOLUTION: In this fermentation process, the cell bodies of Coryneform microorganism are used that bears the polynucleotide sequence selected from the following groups: (a) the polynucleotide that has at least 70% homology to the polynucleotide encoding the specific amino acid sequence originating from Corynebacterium glutamicum; (b) the polynucleotide that encodes the polypeptide including the amino acid sequence that has at least 70% homology to the above-stated amino acid sequence; (c) the polynucleotides that are complementary to the polynucleotides (a) and (b); and (d) the polynucleotides that include at least 15 bases-linking bases in the polynucleotides (a), (b) or (c).