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    REPLICABLE DNA, TRANSFORMED MICROORGANISM, PLASMID VECTOR, AND PRODUCTION OF D-PANTOTHENIC ACID
    4.
    发明专利
    REPLICABLE DNA, TRANSFORMED MICROORGANISM, PLASMID VECTOR, AND PRODUCTION OF D-PANTOTHENIC ACID 失效

    公开(公告)号：JP2000228990A

    公开(公告)日：2000-08-22

    申请号：JP34084099

    申请日：1999-11-30

    Applicant: DEGUSSA

    Inventor： NICOLE DUSCH ,  KALINOWSKI JOERN ,  PUEHLER ALFRED

    IPC: A23K1/16 ,  A23L33/15 ,  C12N1/21 ,  C12N9/88 ,  C12N15/09 ,  C12N15/10 ,  C12N15/52 ,  C12N15/60 ,  C12P13/02 ,  C12R1/15

    Abstract: PROBLEM TO BE SOLVED: To obtain a new replicable DNA which contains a base sequence coding for aspartate-1-decarboxylase, is replicable in a microorganism such as corynebacteria and escherichia, and can be used for producing pantothenic acid by fermentation. SOLUTION: This is a new replicable DNA which consists of a nucleotide sequence consisting of panD gene coding for aspartate-1-decarboxylase, or a sequence corresponding to the sequence shown by the formula within the homology of the genetic code, sequences which hybridizes with sequences complementary to these sequences, is replicable in a microorganism such as corynebacteria and escherichia, can be a recombinant new DNA, and can be used for improved production of pantothenic acid by fermentation and so on. This DNA is obtained by extracting a chromosomal DNA from Corynebacterium glutamicum ATCC 13032, preparing a gene library using the obtained DNA by the conventional method, followed by screening the obtained library using a probe consisting of its partial sequence.

    NUCLEOTIDE SEQUENCES WHICH CODE FOR THE METH GENE
    5.
    发明申请
    NUCLEOTIDE SEQUENCES WHICH CODE FOR THE METH GENE 审中-公开
    核苷酸序列，其代码为甲基

    公开(公告)号：WO0210209A8

    公开(公告)日：2002-04-04

    申请号：PCT/EP0108220

    申请日：2001-07-17

    Applicant: DEGUSSA

    Inventor： BATHE BRIGITTE ,  MOECKEL BETTINA ,  PFEFFERLE WALTER ,  HUTHMACHER KLAUS ,  RUECKERT CHRISTIAN ,  KALINOWSKI JOERN ,  PUEHLER ALFRED ,  BINDER MICHAEL ,  GREISSINGER DIETER ,  THIERBACH GEORG

    IPC: A23K1/16 ,  C12N1/21 ,  C12N9/10 ,  C12P13/12 ,  C07K14/34 ,  A23L1/305 ,  C12N15/10 ,  C12N15/63 ,  C12P13/08 ,  C12Q1/68

    CPC classification number: C12N9/1007 ,  A23K20/142 ,  C12P13/12 ,  C12R1/15 ,  C12R1/19

    Abstract: The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70 % to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70 % to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the metH gene coding for 5 -methyltetrahydrofolate homocysteine methyltransferase (EC 2.1.1.13) is present in enhanced form, and the use of the polynucleotide sequences as hybridization probes.

    Abstract translation: 本发明涉及一种分离的多核苷酸，其包含多核苷酸序列，所述多核苷酸序列选自a）与编码多肽的多核苷酸的程度相同的多核苷酸，所述多核苷酸包含SEQ ID NO： 2，b）编码多肽的多核苷酸，其包含与至少70％与SEQ ID No.2的氨基酸序列相同程度的氨基酸序列，c）多核苷酸，其与 ）或b），和d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少metH基因 编码5-甲基四氢叶酸高半胱氨酸甲基转移酶（EC 2.1.1.13）以增强形式存在，并且使用多核苷酸序列作为杂交探针。

    NUCLEOTIDE SEQUENCES WHICH CODE FOR THE METR AND METZ GENES
    6.
    发明申请
    NUCLEOTIDE SEQUENCES WHICH CODE FOR THE METR AND METZ GENES 审中-公开
    METR和METZ基因编码的核苷酸序列

    公开(公告)号：WO0218430A3

    公开(公告)日：2002-07-04

    申请号：PCT/EP0108221

    申请日：2001-07-17

    Applicant: DEGUSSA

    Inventor： BATHE BRIGITTE ,  PFEFFERLE WALTER ,  HUTHMACHER KLAUS ,  RUECKERT CHRISTIAN ,  KALINOWSKI JOERN ,  PUEHLER ALFRED ,  BINDER MICHAEL ,  GREISSINGER DIETER ,  THIERBACH GEORG

    IPC: C07K14/34 ,  C12N9/88 ,  C12P13/08 ,  C12P13/12

    CPC classification number: C12P13/12 ,  C07K14/34 ,  C12N9/88 ,  C12P13/08

    Abstract: The invention relates to polynucleotides from coryneform bacteria which code for the metR and/or metZ genes and comprise polynucleotide sequences, chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70 % to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which is identical to the extent of at least 70 % to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 3, c) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70 % to the amino acid sequence of SEQ ID No. 2, d) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70 % to the amino acid sequence of SEQ ID No. 3, e) polynucleotide which is complementary to the polynucleotides of a) b), c) or d), and f) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequences of a), b), c), d) or e) and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the metR gene and/or the metZ gene is present in attenuated form, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.

    Abstract translation: 本发明涉及编码metR和/或metZ基因的来自棒状细菌的多核苷酸，其包含选自以下的多核苷酸序列：a）与编码多核苷酸的多核苷酸至少70％相同的多核苷酸， 包含SEQ ID No.2的氨基酸序列的多肽，b）与编码包含SEQ ID No.3的氨基酸序列的多肽的多核苷酸的至少70％同一性的多核苷酸，c ）多核苷酸，其编码包含与SEQ ID No.2的氨基酸序列的至少70％的同一性的氨基酸序列的多肽，d）编码包含氨基酸序列的多肽的多核苷酸 其与SEQ ID No.3的氨基酸序列的至少70％的同一性程度相同，e）与a）b），c）或d）和f）多核苷酸的多核苷酸互补的多核苷酸 其包含a），b），c），d）或e）的多核苷酸序列的至少15个连续核苷酸以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少该metR基因和/或 metZ基因以减毒形式存在，并且使用包含根据本发明的序列作为杂交探针的多核苷酸。

    NEW NUCLEOTIDE SEQUENCE ENCODING lrp GENE
    9.
    发明专利
    NEW NUCLEOTIDE SEQUENCE ENCODING lrp GENE 失效

    公开(公告)号：JP2001128692A

    公开(公告)日：2001-05-15

    申请号：JP2000303892

    申请日：2000-10-03

    Applicant: DEGUSSA

    Inventor： BATHE BRIGITTE ,  KALINOWSKI JOERN ,  PUEHLER ALFRED ,  MOECKEL BETTINA ,  PFEFFERLE WALTER

    IPC: C12N15/09 ,  C07K14/34 ,  C12N1/21 ,  C12N15/31 ,  C12N15/54 ,  C12P13/04 ,  C12P13/08 ,  C12R1/01 ,  C12R1/15

    Abstract: PROBLEM TO BE SOLVED: To provide an improved fermentative method for producing an L- amino acid, especially L-lysine and L-isoleucine by using a new nuclestide encoding lrp gene and a coryneform bacterium. SOLUTION: A polynucleotide is isolated from coryneform bacterium containing (a) a polynucleotide having at least 70% homology to a polynucleotide encoding a polypeptide having a specific amino acid sequence derived from Corynebacterium glutamicum, (b) a polynucleotide encoding a polynucleotide containing an amino acid sequence having at least 70% homology to the amino acid sequence, (c) a polynucleotide complementary to the polynucleotide of (a) or (b) and (d) a polynucleotide sequence selected from a polynucleotide having at least 15 continuous bases of the polynucleotide sequence of (a), (b) or (c). The fermentative method for producing an L-amino acid comprises using amplification or attenuation of the lrp gene.

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