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    TOP-DOWN ANALYSIS OF ANTIBODIES IN MASS SPECTROMETRY
    1.
    发明申请
    TOP-DOWN ANALYSIS OF ANTIBODIES IN MASS SPECTROMETRY 审中-公开

    公开(公告)号：WO2019197983A1

    公开(公告)日：2019-10-17

    申请号：PCT/IB2019/052906

    申请日：2019-04-09

    Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

    Inventor： BABA, Takashi ,  RYUMIN, Pavel ,  LOYD, William M.

    IPC: G01N33/68 ,  G16B40/10 ,  G16B30/00

    Abstract: A separation device separates an unknown intact mAb or reduced mAb subunits of a known mAb class from a sample. An ion source device ionizes the mAb. A mass spectrometer fragments the ionized mAb using an ECD device and mass analyzes resulting product ions using a mass analyzer, producing one or more product ion spectra. Theoretical product ion peaks are calculated for one or more constant portions of the mAb class. The theoretical product ion peaks are removed from the one or more product ion spectra, producing one or more difference product ion spectra. De novo sequencing is applied to the one or more difference product ion spectra, producing one or more candidate sequences for one or more variable portions of the mAb. A genome database is searched for matches to the one or more candidate sequences, producing one or more matched sequences for the one or more variable portions.

    TOF MASS CALIBRATION
    2.
    发明申请
    TOF MASS CALIBRATION 审中-公开

    公开(公告)号：WO2020250157A1

    公开(公告)日：2020-12-17

    申请号：PCT/IB2020/055464

    申请日：2020-06-10

    Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

    Inventor： HAUFLER, Robert E. ,  LOYD, William M. ,  BABA, Takashi

    IPC: G01N30/72 ,  H01J49/00 ,  H01J49/34

    Abstract: A calibration apparatus for a mass analyzer includes an ion source device and a dual-purpose electron beam generating unit. The ion source device ionizes an analyte of a sample, producing analyte ions. The dual-purpose electron beam generating unit is positioned between the ion source device and the mass analyzer. In a first mode, the dual-purpose electron beam generating unit is used to create fragments of analyte ions of unknown mass-to-charge ratio. In a second mode, the dual-purpose electron beam generating unit is used to create ions of calibration compounds of known mass-to-charge ratio. All ions are subsequently transferred to the mass analyzer.

    METHOD FOR TOP DOWN PROTEOMICS USING EXD AND PTR
    3.
    发明申请
    METHOD FOR TOP DOWN PROTEOMICS USING EXD AND PTR 审中-公开

    公开(公告)号：WO2020044160A1

    公开(公告)日：2020-03-05

    申请号：PCT/IB2019/056936

    申请日：2019-08-15

    Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

    Inventor： BABA, Takashi ,  RYUMIN, Pavel ,  LOYD, William M.

    IPC: H01J49/42 ,  H01J49/00

    Abstract: A dissociation device fragments a precursor ion, producing at least two different product ions with overlapping m/z values in the dissociation device. The dissociation device applies an AC voltage and a DC voltage creating a pseudopotential that traps ions below a threshold m/z including the at least two product ions. The dissociation device receives a charge reducing reagent that causes the trapped at least two product ions to be charge reduced until their m/z values increase above the threshold m/z set by the AC voltage. The increase in the m/z values of the at least two product ions decreases their overlap. The at least two product ions with increased m/z values are transmitted to another device for subsequent mass analysis by applying the DC voltage to the dissociation device relative to a DC voltage applied to the other device.

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