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公开(公告)号：WO2022029500A1
公开(公告)日：2022-02-10
申请号：PCT/IB2021/000551
申请日：2021-08-06
Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD. , BLOOMFIELD, Nic , IVOSEV, Gordana , RYUMIN, Pavel
Inventor： BLOOMFIELD, Nic , IVOSEV, Gordana , RYUMIN, Pavel
IPC: H01J49/00 , H01J49/0027 , H01J49/0036 , H01J49/004
Abstract: A method for assigning charge state in mass spectrometry includes receiving a detector response signal corresponding to a plurality of ion arrival events. The detector response signal includes information related to individual ion responses generated by a detector for each ion arrival event. Detector response profiles are generated for mass-to-charge (m/z) bins of a mass spectrum generated from the ion arrival events based on the detector response signal. The m/z bins are grouped into a plurality of groups based on a similarity of the detector response profiles of the m/z bins. A charge state is assigned to one or more features based on the groups of m/z bins.
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公开(公告)号：WO2023012702A1
公开(公告)日：2023-02-09
申请号：PCT/IB2022/057225
申请日：2022-08-03
Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
Inventor： RYUMIN, Pavel , BLOOMFIELD, Nic G. , CHERNUSHEVICH, Igor
Abstract: A mass spectrometer that includes a mass filter and a TOF mass analyzer receives the ion beam from an ion source device that ionizes a compound of a sample. The mass filter selects a precursor ion mass range and the mass analyzer mass analyzes the mass range. A continuous flow of selected precursor ions is maintained between the mass filter and the mass analyzer. A first set of parameters is applied to the mass spectrometer to produce a resolution above a first resolution threshold. A space charge effect is detected by determining if the measured TIC exceeds a TIC threshold or the measured resolution is less than the first resolution threshold. If a space charge effect is detected, at least one precursor ion transmission window with a width smaller than the mass range is applied to the ion beam by the mass filter and mass analyzed to reduce the space charge.
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公开(公告)号：WO2021229301A1
公开(公告)日：2021-11-18
申请号：PCT/IB2021/000330
申请日：2021-05-14
Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
Inventor： RYUMIN, Pavel
Abstract: Methods and systems for identifying or classifying charge states of detected ions. An example method for classifying a charge state of detected ions may include generating a pulse for each ion in a plurality of ions detected by a detector, wherein each pulse has a pulse characteristic (for example a pulse height, width, or area); generating a pulse-characteristic distribution of the generated pulses; and based on the pulse-characteristic distribution, generating an identification of the charge state of one or more ions in the plurality of ions.
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公开(公告)号：WO2020044160A1
公开(公告)日：2020-03-05
申请号：PCT/IB2019/056936
申请日：2019-08-15
Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
Inventor： BABA, Takashi , RYUMIN, Pavel , LOYD, William M.
Abstract: A dissociation device fragments a precursor ion, producing at least two different product ions with overlapping m/z values in the dissociation device. The dissociation device applies an AC voltage and a DC voltage creating a pseudopotential that traps ions below a threshold m/z including the at least two product ions. The dissociation device receives a charge reducing reagent that causes the trapped at least two product ions to be charge reduced until their m/z values increase above the threshold m/z set by the AC voltage. The increase in the m/z values of the at least two product ions decreases their overlap. The at least two product ions with increased m/z values are transmitted to another device for subsequent mass analysis by applying the DC voltage to the dissociation device relative to a DC voltage applied to the other device.
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公开(公告)号：WO2020075069A1
公开(公告)日：2020-04-16
申请号：PCT/IB2019/058559
申请日：2019-10-08
Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
Inventor： RYUMIN, Pavel , BABA, Takashi
Abstract: An electron-ion interaction module for use in a mass spectrometer, having a plurality of rod sets arranged relative to one another such that said rod sets share a common longitudinal axis and each of said rod sets is longitudinally separated from an adjacent rod set by a gap, each of said rod sets comprising a plurality of rods arranged around said common longitudinal axis. The module further includes at least one magnet disposed around said rod sets so as to at least partially surround one or more of said plurality of rod sets and configured to generate a static magnetic field along said longitudinal axis. The rod sets are configured to receive electrons from an electron source and ions from an ion source within an interaction volume defined by the rods. One or more RF voltage sources coupled to the plurality of rod sets applies voltages to the rods.
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Patent Agency Ranking
公开(公告)号：WO2020075068A1
公开(公告)日：2020-04-16
申请号：PCT/IB2019/058558
申请日：2019-10-08
Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
Inventor： BABA, Takashi , RYUMIN, Pavel
Abstract: In one aspect, an electron-ion reaction module, e.g., an electron capture dissociation module, for use in a mass spectrometer is disclosed, which comprises a chamber, an electron source for generating electrons and introducing the electrons into the chamber, a gate electrode positioned relative to the electron source and the chamber, and a DC voltage source operatively coupled to the gate electrode for applying control voltages to the gate electrode. The electron-ion interaction module can further include a controller operably coupled to the DC voltage source and configured for adjusting the DC voltage applied to the gate electrode to adjust flow of electrons into the chamber.
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公开(公告)号：WO2019197983A1
公开(公告)日：2019-10-17
申请号：PCT/IB2019/052906
申请日：2019-04-09
Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
Inventor： BABA, Takashi , RYUMIN, Pavel , LOYD, William M.
Abstract: A separation device separates an unknown intact mAb or reduced mAb subunits of a known mAb class from a sample. An ion source device ionizes the mAb. A mass spectrometer fragments the ionized mAb using an ECD device and mass analyzes resulting product ions using a mass analyzer, producing one or more product ion spectra. Theoretical product ion peaks are calculated for one or more constant portions of the mAb class. The theoretical product ion peaks are removed from the one or more product ion spectra, producing one or more difference product ion spectra. De novo sequencing is applied to the one or more difference product ion spectra, producing one or more candidate sequences for one or more variable portions of the mAb. A genome database is searched for matches to the one or more candidate sequences, producing one or more matched sequences for the one or more variable portions.
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公开(公告)号：WO2023026201A2
公开(公告)日：2023-03-02
申请号：PCT/IB2022/057908
申请日：2022-08-23
Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
Inventor： BABA, Takashi , FAUR, Manuel , GERA, Tiberiu , HAUFLER, Robert , LOYD, William , RYUMIN, Pavel , YOU, Congsheng
Abstract: A system for applying RF voltages to a multipole ion processing device, configured for use in a mass spectrometer, includes a first RF generator configured to generate a first RF voltage and apply to a first pole electrode set, a second RF generator configured to generate a second RF voltage and apply to a second pole electrode set, a first amplitude adjustor configured to adjust an amplitude of the first RF voltage, a second amplitude adjustor configure to adjust an amplitude of the second RF voltage, and a phase adjustor in communication with the first RF generator and the second RF generator to adjust phase output of at least one of the first RF generator and the second RF generator so as to adjust a phase differential between the first RF voltage and the second RF voltage to be within a desired range.
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公开(公告)号：WO2023275693A1
公开(公告)日：2023-01-05
申请号：PCT/IB2022/055906
申请日：2022-06-24
Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
Inventor： CAMPBELL, John Lawrence , LOYD, Bill , RYUMIN, Pavel
IPC: H01J49/00 , H01J49/0054
Abstract: The present disclosure provides methods and systems for performing mass spectrometry in which at least two batches of precursor ions generated via ionization of at least two different portions of a sample are exposed to electron beams at different energies to cause fragmentation of at least a portion of the precursor ions. In some embodiments, the electron energies can be selected such at one of the electron energies, EIEIO fragmentation can occur while at the other electron energy, EIEO fragmentation channel is not available. The mass spectra corresponding to the two energies can then be utilized to generate a resultant mass spectrum in which mass peaks corresponding to ion fragments generated by EIEIO dissociation are more readily identifiable.
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10.发明申请
公开(公告)号：WO2020157720A1
公开(公告)日：2020-08-06
申请号：PCT/IB2020/050795
申请日：2020-01-31
Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
Inventor： RYUMIN, Pavel , BABA, Takashi , BLOOMFIELD, Nic G.
IPC: H01J49/00
Abstract: Intensity measurements made by electron multiplier and image-charge detectors are proportional to charge state. These intensities are used to separate detected ions into different data sets and create mass spectra from the different data sets. Ion measurements are separated by charge state using (i) a single electron multiplier detector, (ii) a single image-charge detector, or (iii) multiple electron multiplier ADC detectors. Using (i), the intensity of a peak calculated from each measured pulse is compared to predetermined intensity ranges and each peak is stored in a corresponding data set. Using (ii), each measured transient time-domain signal is converted to frequency-domain peaks, the intensity of each frequency-domain peak is compared to predetermined intensity ranges, and each peak is stored in a corresponding data set. Using (iii), each detector is adapted to measure a predetermined intensity range and store calculated peaks from the measured pulses in corresponding data sets.
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