公开(公告)号：EP0516814B1

公开(公告)日：1996-09-04

申请号：EP92902996.5

申请日：1991-12-23

Applicant: Dade MicroScan Inc.

Inventor： BADAL, Robert ,  KELLEY, Roger ,  SAND, Theodore, T. ,  BASCOMB, Shoshana

IPC: C12Q1/18

CPC classification number: C12Q1/04 ,  C12Q1/18 ,  C12Q2337/22 ,  Y10S435/968 ,  Y10S436/80

Abstract: This invention relates to a method to determine susceptibility to antimicrobial agents of a majority of clinically significant Gram positive organisms. This invention also relates to a mixture of fluorogenic substrates used to detect the growth of Gram positive Bacteria.

公开(公告)号：EP0538447B1

公开(公告)日：1997-05-28

申请号：EP92911242.3

申请日：1992-04-29

Applicant: Dade MicroScan Inc.

Inventor： JOHNSTON, Judith ,  FELLAND, Tracy ,  BASCOMB, Shoshana ,  GODSEY, James, H.

IPC: C12Q1/02 ,  C12Q1/18 ,  C12Q1/20 ,  C12N1/38 ,  C12Q1/34

CPC classification number: C12Q1/18 ,  Y10S435/975

Abstract: In an assay for determination of beta-lactam antibiotic resistance in a target bacterial strain, the strain is grown in the presence of both a beta-lactamase inducing antibiotic and a beta-lactam indicator antibiotic which kills or inhibits the growth of bacteria unable to hydrolyze beta-lactam antibiotics. Growth, indicative of drug resistance in the target strain, is monitored by detecting a fluorophor released by the enzymatic cleavage of a metabolizable fluorogenic compound.