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公开(公告)号：EP0538447B1
公开(公告)日：1997-05-28
申请号：EP92911242.3
申请日：1992-04-29
Applicant: Dade MicroScan Inc.
Inventor： JOHNSTON, Judith , FELLAND, Tracy , BASCOMB, Shoshana , GODSEY, James, H.
CPC classification number: C12Q1/18 , Y10S435/975
Abstract: In an assay for determination of beta-lactam antibiotic resistance in a target bacterial strain, the strain is grown in the presence of both a beta-lactamase inducing antibiotic and a beta-lactam indicator antibiotic which kills or inhibits the growth of bacteria unable to hydrolyze beta-lactam antibiotics. Growth, indicative of drug resistance in the target strain, is monitored by detecting a fluorophor released by the enzymatic cleavage of a metabolizable fluorogenic compound.
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