公开(公告)号：WO2014194417A1

公开(公告)日：2014-12-11

申请号：PCT/CA2014/050496

申请日：2014-05-28

Applicant: FLUIDIGM CANADA INC.

Inventor： TANNER, Scott

IPC: H01J49/40 ,  H01J49/06 ,  H03M1/12

CPC classification number: H01J49/40 ,  H01J49/025

Abstract: A mass spectrometer of the type useful in mass cytometry includes an ion detector. A digitizing system for converting analog signals from the ion detector includes two analog-to-digital converters. The analog-to-digital converters are configured to provide an increased dynamic range for a targeted period while limiting the amount of data generated.

Abstract translation: 可用于质量细胞计数的质谱仪包括离子检测器。 用于转换离子检测器的模拟信号的数字化系统包括两个模拟 - 数字转换器。 模数转换器被配置为为目标周期提供增加的动态范围，同时限制所生成的数据量。

公开(公告)号：WO2014190429A1

公开(公告)日：2014-12-04

申请号：PCT/CA2014/050474

申请日：2014-05-22

Applicant: FLUIDIGM CANADA INC.

Inventor： BANDURA, Dmitry ,  BARANOV, Vladimir ,  LOBODA, Alexander ,  ORNATSKY, Olga ,  TANNER, Scott

IPC: G01N27/62 ,  G01N33/483

CPC classification number: G01N33/54366 ,  G01N15/1031 ,  G01N15/1404 ,  G01N15/1459 ,  G01N27/622 ,  G01N27/624 ,  G01N33/483 ,  G01N33/532 ,  G01N33/54313 ,  G01N2015/1006 ,  G01N2458/15

Abstract: The invention relates to methods and devices for molecular cytometry. The molecular cytometer is for use in the analysis of molecular tag labeled particles. In the molecular cytometer, volatile molecular tags attached to the particles, such as cells, are released as groups of molecular tags and ionized by the method of soft ionization to produce corresponding groups of molecular ions. The molecular cytometer has two detectors, one to detect the presence of the groups of molecular ions and the other to detect mobility separated molecular ions. The mobility separated molecular ions are synchronized to correspond with the groups of molecular ions.

Abstract translation: 本发明涉及用于分子细胞计数的方法和装置。 分子光谱仪用于分子标签标记颗粒的分析。 在分子细胞仪中，附着于粒子（如细胞）的挥发性分子标记作为分子标签的基团释放，并通过软电离方法离子化以产生相应的分子离子组。 分子细胞仪有两个检测器，一个用于检测分子离子组的存在，另一个用于检测迁移率分离的分子离子。 迁移率分离的分子离子被同步以对应于分子离子的组。

公开(公告)号：WO2014063246A1

公开(公告)日：2014-05-01

申请号：PCT/CA2013/050797

申请日：2013-10-22

Applicant: FLUIDIGM CANADA INC.

Inventor： TANNER, Scott

IPC: G01N27/00 ,  C12M1/34 ,  G01N33/483 ,  G01N33/58 ,  C40B20/08 ,  C40B70/00 ,  H01J49/40

CPC classification number: G01N27/626 ,  G01N1/04 ,  G01N2001/045 ,  H01J49/0031 ,  H01J49/0418

Abstract: In a mass cytometer system, a tissue sample labeled with multiple metal tags is supported on an encoded substrate for distribution profile mapping by laser ablation. Groups of elemental ions from each plume generated by each laser pulse are detected by the mass cytometer and the data is mapped according to the encoded substrate. This configuration allows for the production of a 3-dimentional distribution profile of the multiple metal tags in the tissue sample.

Abstract translation: 在质谱仪系统中，通过激光烧蚀在经编码的衬底上支持用多个金属标签标记的组织样品用于分布轮廓图。 通过质谱仪检测由每个激光脉冲产生的每个羽流的元素离子组，并且根据编码的底物映射数据。 这种构造允许生成组织样品中多个金属标签的3维分布轮廓。

公开(公告)号：EP3005404B1

公开(公告)日：2019-08-28

申请号：EP14807677.1

申请日：2014-05-28

Applicant: Fluidigm Canada Inc.

Inventor： TANNER, Scott

IPC: H01J49/02 ,  H01J49/40

公开(公告)号：EP3591688A1

公开(公告)日：2020-01-08

申请号：EP19193863.8

申请日：2014-05-28

Applicant: Fluidigm Canada Inc.

Inventor： TANNER, Scott

IPC: H01J49/02

Abstract: A mass spectrometer of the type useful in mass cytometry includes an ion detector. A digitizing system for converting analog signals from the ion detector includes two analog-to-digital converters. The analog-to-digital converters are configured to provide an increased dynamic range for a targeted period while limiting the amount of data generated.

公开(公告)号：EP3005404A1

公开(公告)日：2016-04-13

申请号：EP14807677.1

申请日：2014-05-28

Applicant: Fluidigm Canada Inc.

Inventor： TANNER, Scott

IPC: H01J49/40 ,  H01J49/06 ,  H03M1/12

CPC classification number: H01J49/40 ,  H01J49/025

Abstract: A mass spectrometer of the type useful in mass cytometry includes an ion detector. A digitizing system for converting analog signals from the ion detector includes two analog-to-digital converters. The analog-to-digital converters are configured to provide an increased dynamic range for a targeted period while limiting the amount of data generated.

Abstract translation: 可用于质量细胞计数的质谱仪包括离子检测器。 用于转换离子检测器的模拟信号的数字化系统包括两个模拟 - 数字转换器。 模数转换器被配置为为目标周期提供增加的动态范围，同时限制所生成的数据量。

公开(公告)号：EP3004860A1

公开(公告)日：2016-04-13

申请号：EP14804873.9

申请日：2014-05-22

Applicant: Fluidigm Canada Inc.

Inventor： BANDURA, Dmitry ,  BARANOV, Vladimir ,  LOBODA, Alexander ,  ORNATSKY, Olga ,  TANNER, Scott

IPC: G01N27/62 ,  G01N33/483

CPC classification number: G01N33/54366 ,  G01N15/1031 ,  G01N15/1404 ,  G01N15/1459 ,  G01N27/622 ,  G01N27/624 ,  G01N33/483 ,  G01N33/532 ,  G01N33/54313 ,  G01N2015/1006 ,  G01N2458/15

Abstract: The invention relates to methods and devices for molecular cytometry. The molecular cytometer is for use in the analysis of molecular tag labeled particles. In the molecular cytometer, volatile molecular tags attached to the particles, such as cells, are released as groups of molecular tags and ionized by the method of soft ionization to produce corresponding groups of molecular ions. The molecular cytometer has two detectors, one to detect the presence of the groups of molecular ions and the other to detect mobility separated molecular ions. The mobility separated molecular ions are synchronized to correspond with the groups of molecular ions.

Abstract translation: 本发明涉及用于分子细胞计数的方法和装置。 分子细胞仪用于分子标签标记颗粒的分析。 在分子细胞仪中，附着于颗粒（如细胞）的挥发性分子标签作为分子标签的基团释放，并通过软电离方法离子化，产生相应的分子离子组。 分子细胞仪有两个检测器，一个用于检测分子离子组的存在，另一个用于检测迁移率分离的分子离子。 迁移率分离的分子离子被同步以与分子离子的组对应。

公开(公告)号：EP2912445A1

公开(公告)日：2015-09-02

申请号：EP13849451.3

申请日：2013-10-22

Applicant: Fluidigm Canada Inc.

Inventor： TANNER, Scott

IPC: G01N27/00 ,  C12M1/34 ,  G01N33/483 ,  G01N33/58 ,  C40B20/08 ,  C40B70/00 ,  H01J49/40

CPC classification number: G01N27/626 ,  G01N1/04 ,  G01N2001/045 ,  H01J49/0031 ,  H01J49/0418

Abstract: In a mass cytometer system, a tissue sample labeled with multiple metal tags is supported on an encoded substrate for distribution profile mapping by laser ablation. Groups of elemental ions from each plume generated by each laser pulse are detected by the mass cytometer and the data is mapped according to the encoded substrate. This configuration allows for the production of a 3-dimentional distribution profile of the multiple metal tags in the tissue sample.

Abstract translation: 在质量流式细胞仪系统中，用多个金属标签标记的组织样品被支撑在编码基底上，用于通过激光烧蚀进行分布轮廓映射。 由每个激光脉冲产生的来自每个羽流的元素离子组由质量细胞仪检测，并且数据根据编码的底物被映射。 该配置允许在组织样本中产生多个金属标签的三维分布轮廓。