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    MOLECULAR CYTOMETRY
    2.
    发明申请
    MOLECULAR CYTOMETRY 审中-公开
    分子细胞计数

    公开(公告)号：WO2014190429A1

    公开(公告)日：2014-12-04

    申请号：PCT/CA2014/050474

    申请日：2014-05-22

    Applicant: FLUIDIGM CANADA INC.

    Inventor： BANDURA, Dmitry ,  BARANOV, Vladimir ,  LOBODA, Alexander ,  ORNATSKY, Olga ,  TANNER, Scott

    IPC: G01N27/62 ,  G01N33/483

    CPC classification number: G01N33/54366 ,  G01N15/1031 ,  G01N15/1404 ,  G01N15/1459 ,  G01N27/622 ,  G01N27/624 ,  G01N33/483 ,  G01N33/532 ,  G01N33/54313 ,  G01N2015/1006 ,  G01N2458/15

    Abstract: The invention relates to methods and devices for molecular cytometry. The molecular cytometer is for use in the analysis of molecular tag labeled particles. In the molecular cytometer, volatile molecular tags attached to the particles, such as cells, are released as groups of molecular tags and ionized by the method of soft ionization to produce corresponding groups of molecular ions. The molecular cytometer has two detectors, one to detect the presence of the groups of molecular ions and the other to detect mobility separated molecular ions. The mobility separated molecular ions are synchronized to correspond with the groups of molecular ions.

    Abstract translation: 本发明涉及用于分子细胞计数的方法和装置。 分子光谱仪用于分子标签标记颗粒的分析。 在分子细胞仪中，附着于粒子（如细胞）的挥发性分子标记作为分子标签的基团释放，并通过软电离方法离子化以产生相应的分子离子组。 分子细胞仪有两个检测器，一个用于检测分子离子组的存在，另一个用于检测迁移率分离的分子离子。 迁移率分离的分子离子被同步以对应于分子离子的组。

    SYSTEMS, METHODS AND COMPOSITIONS FOR SIMULTANEOUS DETECTION OF RNA AND PROTEIN BY MASS SPECTROMETRY
    5.
    发明申请
    SYSTEMS, METHODS AND COMPOSITIONS FOR SIMULTANEOUS DETECTION OF RNA AND PROTEIN BY MASS SPECTROMETRY 审中-公开
    通过质谱法同时检测RNA和蛋白质的系统，方法和组合物

    公开(公告)号：WO2017004203A1

    公开(公告)日：2017-01-05

    申请号：PCT/US2016/040095

    申请日：2016-06-29

    Applicant: FLUIDIGM CANADA INC.

    Inventor： ORNATSKY, Olga

    IPC: C07H19/00 ,  C07H21/02 ,  C07H21/00

    CPC classification number: G01N33/6851 ,  C07H21/00 ,  C07H21/02 ,  C12N2310/3145 ,  C12N2310/315 ,  C12Q1/68 ,  C12Q1/6818 ,  C12Q1/6872 ,  C12Q2565/627 ,  C12Q2600/16

    Abstract: In certain embodiments, a method of detection includes providing cells labeled with a first element tag bound to a target RNA sequence and a second element tag bound to a target protein. The method may further include ionizing a cell, or a portion thereof, to produce an ionized first and second element tag, and simultaneously detecting the ionized first and second element tag. The ionization may be performed by an inductively coupled plasma (ICP) torch. The method may further comprise nebulizing the cells in suspension prior to ionizing. In certain embodiments, the ionized first and second element tag may be detected by time-of-flight mass spectrometry. Additional methods, and systems and reagents for simultaneous detection of RNA and protein by mass spectrometry are also described.

    Abstract translation: 在某些实施方案中，检测方法包括提供标记有与靶RNA序列结合的第一元件标签的细胞和与靶蛋白结合的第二元件标签。 该方法还可以包括电离细胞或其一部分，以产生电离的第一和第二元件标签，同时检测电离的第一和第二元件标签。 电离可以通过电感耦合等离子体（ICP）手电筒进行。 该方法还可以包括在电离之前将悬浮液中的细胞雾化。 在某些实施方案中，可以通过飞行时间质谱法检测电离的第一和第二元件标签。 还描述了用于通过质谱法同时检测RNA和蛋白质的其它方法和系统和试剂。

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