公开(公告)号：CA3027423A1

公开(公告)日：2018-01-18

申请号：CA3027423

申请日：2017-07-12

Applicant: FLUIDIGM CORP

Inventor： LIVAK KENNETH J ,  FEKETE RICHARD A

IPC: C12Q1/68

Abstract: Described herein are methods for preparing DNA templates for single-cell transcript sequencing of RNA from a population of cells. The methods entail distributing cells from the population into separate reaction volumes so that a plurality of separate reaction volumes each contain a single, isolated cell, wherein the cells have been treated with a fixative prior to distribution. The isolated cells are then permeabilized or disrupted, and cDNA is prepared by reverse transcript, followed by amplification. Also provided is a novel chemistry for efficient production of DNA templates from T-cell receptors or immunoglobulins in single cells.

公开(公告)号：SG11201811048UA

公开(公告)日：2019-01-30

申请号：SG11201811048U

申请日：2017-07-12

Applicant: FLUIDIGM CORP

Inventor： LIVAK KENNETH J ,  FEKETE RICHARD A

IPC: C12Q1/68