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    MULTIFUNCTIONAL PROBE-PRIMERS
    1.
    发明申请
    MULTIFUNCTIONAL PROBE-PRIMERS 审中-公开
    多功能探测器

    公开(公告)号：WO2012106668A3

    公开(公告)日：2013-01-10

    申请号：PCT/US2012023870

    申请日：2012-02-03

    Applicant: FLUIDIGM CORP ,  LIVAK KENNETH J

    Inventor： LIVAK KENNETH J

    IPC: C12Q1/68 ,  C07H21/00

    CPC classification number: C12Q1/6876 ,  C12Q1/6818 ,  C12Q1/6823 ,  C12Q2525/161 ,  C12Q2525/197 ,  C12Q2565/1015

    Abstract: Methods and reagents for detection and analysis of nucleic acids are provided. Certain methods involves an encoding amplification in which a target sequence is associated with probe-binding sequences and optionally with indexing sequences, (2) an optional distribution step in which the product of the encoding amplification is split into multiple aliquots, and (3) a decoding and detection step in which the presence, absence, quantity, or relative amount of the target sequence in the aliquots is determined. The detection step makes use of a multifunctional "self-digesting" molecular probe comprising a primer polynucleotide and a probe oligonucleotide, linked in a 5'-5' orientation.

    Abstract translation: 提供了用于检测和分析核酸的方法和试剂。 某些方法涉及编码扩增，其中靶序列与探针结合序列和任选的索引序列相关，（2）任选的分布步骤，其中编码扩增的产物被分成多个等分试样，和（3）a 解码和检测步骤，其中确定等分试样中靶序列的存在，不存在，数量或相对量。 检测步骤使用包含以5'-5'取向连接的引物多核苷酸和探针寡核苷酸的多功能“自消化”分子探针。

    SINGLE-CELL TRANSCRIPT SEQUENCING
    7.
    发明专利
    SINGLE-CELL TRANSCRIPT SEQUENCING 未知

    公开(公告)号：CA3027423A1

    公开(公告)日：2018-01-18

    申请号：CA3027423

    申请日：2017-07-12

    Applicant: FLUIDIGM CORP

    Inventor： LIVAK KENNETH J ,  FEKETE RICHARD A

    IPC: C12Q1/68

    Abstract: Described herein are methods for preparing DNA templates for single-cell transcript sequencing of RNA from a population of cells. The methods entail distributing cells from the population into separate reaction volumes so that a plurality of separate reaction volumes each contain a single, isolated cell, wherein the cells have been treated with a fixative prior to distribution. The isolated cells are then permeabilized or disrupted, and cDNA is prepared by reverse transcript, followed by amplification. Also provided is a novel chemistry for efficient production of DNA templates from T-cell receptors or immunoglobulins in single cells.

    MULTIPLEX AMPLIFICATION FOR THE DETECTION OF NUCLEIC ACID VARIATIONS
    10.
    发明专利
    MULTIPLEX AMPLIFICATION FOR THE DETECTION OF NUCLEIC ACID VARIATIONS 未知

    公开(公告)号：CA2786916A1

    公开(公告)日：2011-07-21

    申请号：CA2786916

    申请日：2011-01-14

    Applicant: UNIV BRITISH COLUMBIA ,  FLUIDIGM CORP

    Inventor： LIVAK KENNETH J ,  HEYRIES KEVIN ,  HANSEN CARL L G ,  PETRIV OLEH

    IPC: C12Q1/68 ,  C12P19/34 ,  C40B30/04 ,  G01N33/50

    Abstract: Kits, primers, and methods are provided herein for detecting relative target source to reference source ratios in a biological sample, by distributing the biological sample into discrete subsamples, wherein the biological sample includes, a plurality of target molecules on a target source; and a plurality of reference molecules on a reference source; providing target primers directed to one or more of the plurality of target molecules and reference primers directed to one or more of the plurality of reference molecules; performing digital amplification with the target primers and the reference primers; and detecting the presence or absence of amplified target products with target probes and detecting the presence or absence of amplified reference products with reference probes, wherein the ratio of amplified target products to amplified reference products is indicative of a relative amount of target source to reference source in a biological sample.

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