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    Thermal reaction device and method for using the same
    1.
    发明专利
    Thermal reaction device and method for using the same 审中-公开
    热反应装置及其使用方法

    公开(公告)号：JP2012085659A

    公开(公告)日：2012-05-10

    申请号：JP2012020787

    申请日：2012-02-02

    Applicant: Fluidigm Corp ,  フルイディグム コーポレイション

    Inventor： GOODSAID FEDERICO ,  UNGER MARC A ,  HUANG JIANG ,  QUAN EMERSON ,  GROSSMAN ROBERT ,  LAM PHILLIP ,  CHOU HOU-PU ,  KIMBALL JAKE ,  PIEPRZYK MARTIN ,  FACER GEOFFREY

    IPC: C12N15/09 ,  C12M1/00 ,  G01N37/00

    Abstract: PROBLEM TO BE SOLVED: To provide devices that can be utilized to conduct a variety of nucleic acid amplification reactions, while having sufficient versatility for use in other types of analyses as well.SOLUTION: An M×N matrix microfluidic device for performing a matrix of reactions is disclosed. This device (100) has a plurality of reaction cells (106) in communication with one of either a sample inlet (120) or a reagent inlet (124) through a via formed within an elastomeric block of the device. Methods provided include a method for forming vias in parallel in an elastomeric layer of the microfluidic device. The method includes a step of using patterned photoresist masks and a step of etching regions or portions of an elastomeric layer of the elastomeric block.

    Abstract translation: 要解决的问题：提供可用于进行各种核酸扩增反应的装置，同时具有用于其它类型分析的足够多功能性。 解决方案：公开了一种用于执行反应矩阵的M×N矩阵微流体装置。 该装置（100）具有通过形成在装置的弹性体块内的通孔与样品入口（120）或试剂入口（124）中的一个连通的多个反应池（106）。 提供的方法包括在微流体装置的弹性体层中平行地形成通孔的方法。 该方法包括使用图案化的光致抗蚀剂掩模和蚀刻弹性体块的弹性层的区域或部分的步骤。 版权所有（C）2012，JPO＆INPIT

    METHOD AND SYSTEM FOR MICROFLUIDIC DEVICE AND IMAGING THEREOF
    3.
    发明申请
    METHOD AND SYSTEM FOR MICROFLUIDIC DEVICE AND IMAGING THEREOF 审中-公开
    微流体装置及其成像方法与系统

    公开(公告)号：WO2004103563A2

    公开(公告)日：2004-12-02

    申请号：PCT/US2004016115

    申请日：2004-05-20

    Applicant: FLUIDIGM CORP ,  QUAN EMERSON ,  TAYLOR COLIN JON ,  LEE MICHAEL ,  CEASAR CHRISTOPHER ,  HARRIS GREG

    Inventor： QUAN EMERSON ,  TAYLOR COLIN JON ,  LEE MICHAEL ,  CEASAR CHRISTOPHER ,  HARRIS GREG

    IPC: B01L3/00 ,  B01L

    CPC classification number: G01N33/00 ,  B01L3/5025 ,  B01L3/502707 ,  B01L2200/025 ,  B01L2200/12 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2400/0481 ,  B01L2400/0655 ,  Y10T436/11

    Abstract: A biological substrate, e.g., microfluidic chip. The substrate includes a rigid substrate material, which has a surface region capable of acting as a handle substrate. The substrate also has a deformable fluid layer coupled to the surface region. One or more well regions are formed in a first portion of the deformable fluid layer and are capable of holding a fluid therein. The one or more channel regions are formed in a second portion of the deformable fluid layer and are coupled to one or more of the well regions. An active region is formed in the deformable fluid layer. At least three fiducial markings are formed within the non-active region and disposed in a spatial manner associated with at least one of the well regions. A control layer is coupled to the fluid layer.

    Abstract translation: 生物底物，例如微流体芯片。 衬底包括刚性衬底材料，其具有能够用作手柄衬底的表面区域。 衬底还具有耦合到表面区域的可变形流体层。 一个或多个阱区域形成在可变形流体层的第一部分中并且能够在其中保持流体。 一个或多个通道区域形成在可变形流体层的第二部分中并且耦合到一个或多个阱区域。 在可变形流体层中形成有源区。 在非有源区域内形成至少三个基准标记，并以与至少一个阱区域相关联的空间方式设置。 控制层耦合到流体层。

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