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公开(公告)号：WO2012162267A2
公开(公告)日：2012-11-29
申请号：PCT/US2012/038894
申请日：2012-05-21
Applicant: FLUIDIGM CORPORATION , ANDERSON, Megan , CHEN, Peilin , FOWLER, Brian , JONES, Robert C. , KAPER, Fiona , LEBOFSKY, Ronald , MAY, Andrew
Inventor： ANDERSON, Megan , CHEN, Peilin , FOWLER, Brian , JONES, Robert C. , KAPER, Fiona , LEBOFSKY, Ronald , MAY, Andrew
CPC classification number: C12Q1/6855 , C12N15/10 , C12N15/1065 , C12N15/65 , C12N15/66 , C12Q1/686 , C12Q1/6874
Abstract: Described herein are methods useful for incorporating one or more adaptors and/or nucleotide tag(s) and/or barcode nucleotide sequence(s) one, or typically more, target nucleotide sequences. In particular embodiments, nucleic acid fragments having adaptors, e.g., suitable for use in high-throughput DNA sequencing are generated. In other embodiments, information about a reaction mixture is encoded into a reaction product. Also described herein are methods and kits useful for amplifying one or more target nucleic acids in preparation for applications such as bidirectional nucleic acid sequencing. In particular embodiments, methods of the invention entail additionally carrying out bidirectional DNA sequencing. Also described herein are methods for encoding and detecting and/or quantifying alleles by primer extension.
Abstract translation: 本文所描述的方法可用于掺入一个或多个衔接子和/或核苷酸标签和/或条形码核苷酸序列，一个或多个靶核苷酸序列。 在具体实施方案中，产生具有适配器（例如适合用于高通量DNA测序）的核酸片段。 在其它实施方案中，关于反应混合物的信息被编码成反应产物。 本文还描述了可用于扩增一种或多种靶核酸以准备应用如双向核酸测序的方法和试剂盒。 在具体实施方案中，本发明的方法还需要进行双向DNA测序。 本文还描述了通过引物延伸来编码和检测和/或定量等位基因的方法。
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2.发明申请SIMULTANEOUS DETECTION OF TARGET PROTEIN AND TARGET NUCLEIC ACIDS IN A SINGLE CELL 审中-公开目标蛋白和目标核酸在单细胞中的同时检测
公开(公告)号：WO2014144371A1
公开(公告)日：2014-09-18
申请号：PCT/US2014/028751
申请日：2014-03-14
Applicant: FLUIDIGM CORPORATION
Inventor： JONES, Robert C.
IPC: C12Q1/68
CPC classification number: C12Q1/686 , C12Q1/682 , G01N33/532 , G01N2458/10 , C12Q2521/101 , C12Q2531/113 , C12Q2537/125 , C12Q2545/114
Abstract: Methods and reagents for detection and analysis of nucleic acids are provided. The methods employ proximity extension assays for detection of a target nucleic acids of interest, e.g., a target RNA. The method can additionally be used in multiplex assays with a protein proximity extension assay to detect protein.
Abstract translation: 提供了用于检测和分析核酸的方法和试剂。 所述方法使用用于检测目标靶核酸的接近延伸测定法，例如靶RNA。 该方法可以另外用于具有蛋白质接近延伸测定的多重测定中以检测蛋白质。
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Patent Agency Ranking
公开(公告)号：WO2008067552A2
公开(公告)日：2008-06-05
申请号：PCT/US2007/086146
申请日：2007-11-30
Applicant: FLUIDIGM CORPORATION , SUN, Gang , RAMAKRISHNAN, Ramesh , JONES, Robert C.
Inventor： SUN, Gang , RAMAKRISHNAN, Ramesh , JONES, Robert C.
IPC: G06T11/20
CPC classification number: C12Q1/686 , C12Q2565/629 , C12Q2565/501 , C12Q2545/114
Abstract: A method of adjusting amplification curves in a PCR experiment includes receiving a plurality of amplification curves for a sample and computing a first parameter for each of the plurality of amplification curves. The method also includes computing a second parameter for each of the plurality of amplification curves and computing a third parameter using at least a portion of the first or second parameters. The method further includes computing an offset for each of the plurality of amplification curves. The offset is a function of the first parameter and the third parameter. Moreover, the method includes adjusting at least one of the plurality of amplification curves by subtracting the offset.
Abstract translation: 在PCR实验中调整扩增曲线的方法包括接收样品的多个放大曲线并计算多个放大曲线中的每一个的第一参数。 该方法还包括为多个放大曲线中的每一个计算第二参数，并使用第一或第二参数的至少一部分来计算第三参数。 该方法还包括计算多个放大曲线中的每一个的偏移量。 偏移量是第一个参数和第三个参数的函数。 此外，该方法包括通过减去偏移来调整多个放大曲线中的至少一个。
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公开(公告)号：EP2710172B1
公开(公告)日：2017-03-29
申请号：EP12789957.3
申请日：2012-05-21
Applicant: Fluidigm Corporation
Inventor： ANDERSON, Megan , CHEN, Peilin , FOWLER, Brian , JONES, Robert C. , KAPER, Fiona , LEBOFSKY, Ronald , MAY, Andrew
CPC classification number: C12Q1/6855 , C12N15/10 , C12N15/1065 , C12N15/65 , C12N15/66 , C12Q1/686 , C12Q1/6874
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公开(公告)号：EP2710172A2
公开(公告)日：2014-03-26
申请号：EP12789957.3
申请日：2012-05-21
Applicant: Fluidigm Corporation
Inventor： ANDERSON, Megan , CHEN, Peilin , FOWLER, Brian , JONES, Robert C. , KAPER, Fiona , LEBOFSKY, Ronald , MAY, Andrew
CPC classification number: C12Q1/6855 , C12N15/10 , C12N15/1065 , C12N15/65 , C12N15/66 , C12Q1/686 , C12Q1/6874
Abstract: Described herein are methods useful for incorporating one or more adaptors and/or nucleotide tag(s) and/or barcode nucleotide sequence(s) one, or typically more, target nucleotide sequences. In particular embodiments, nucleic acid fragments having adaptors, e.g., suitable for use in high-throughput DNA sequencing are generated. In other embodiments, information about a reaction mixture is encoded into a reaction product. Also described herein are methods and kits useful for amplifying one or more target nucleic acids in preparation for applications such as bidirectional nucleic acid sequencing. In particular embodiments, methods of the invention entail additionally carrying out bidirectional DNA sequencing. Also described herein are methods for encoding and detecting and/or quantifying alleles by primer extension.
Abstract translation: 本文描述的方法可用于掺入一个或多个衔接子和/或核苷酸标签和/或条形码核苷酸序列，一个或多个靶核苷酸序列。 在具体实施方案中，产生具有适配器（例如适合用于高通量DNA测序）的核酸片段。 在其它实施方案中，关于反应混合物的信息被编码成反应产物。 本文还描述了可用于扩增一种或多种靶核酸以准备应用如双向核酸测序的方法和试剂盒。 在具体实施方案中，本发明的方法还需要进行双向DNA测序。 本文还描述了通过引物延伸来编码和检测和/或定量等位基因的方法。
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6.发明公开SIMULTANEOUS DETECTION OF TARGET PROTEIN AND TARGET NUCLEIC ACIDS IN A SINGLE CELL 审中-公开同时检测单细胞中的靶标蛋白和靶核酸
公开(公告)号：EP2971121A1
公开(公告)日：2016-01-20
申请号：EP14763446.3
申请日：2014-03-14
Applicant: Fluidigm Corporation
Inventor： JONES, Robert C.
IPC: C12Q1/68
CPC classification number: C12Q1/686 , C12Q1/682 , G01N33/532 , G01N2458/10 , C12Q2521/101 , C12Q2531/113 , C12Q2537/125 , C12Q2545/114
Abstract: Methods and reagents for detection and analysis of nucleic acids are provided. The methods employ proximity extension assays for detection of a target nucleic acids of interest, e.g., a target RNA. The method can additionally be used in multiplex assays with a protein proximity extension assay to detect protein.
Abstract translation: 提供了用于检测和分析核酸的方法和试剂。 该方法使用邻近延伸测定法来检测感兴趣的靶核酸，例如靶RNA。 该方法还可以用于多重分析中，使用蛋白质邻近延伸分析来检测蛋白质。
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公开(公告)号：EP2569447A2
公开(公告)日：2013-03-20
申请号：EP11780930.1
申请日：2011-05-16
Applicant: Fluidigm Corporation , Pieprzyk, Martin
Inventor： PIEPRZYK, Martin , JONES, Robert C. , LIVAK, Kenneth J. , MAY, Andrew , MIR, Alain , QIN, Jian , RAMAKRISHNAN, Ramesh , SPURGEON, Sandra , WANG, Jun , ZIMMERMANN, Bernhard G.
CPC classification number: C12Q1/6851 , C12Q1/6855 , C12Q1/6858 , G01N33/542 , G01N33/582 , G01N33/6893 , G01N2800/368 , C12Q2521/301 , C12Q2521/501 , C12Q2525/155 , C12Q2535/131 , C12Q2537/143 , C12Q2537/16 , C12Q2563/173
Abstract: The present invention provides amplification-based methods for detection of genotype, mutations, and/or aneuploidy. These methods have broad applicability, but are particularly well-suited to detecting and quantifying target nucleic acids in free fetal DNA present in a maternal bodily fluid sample.
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