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公开(公告)号：WO2019036723A1
公开(公告)日：2019-02-21
申请号：PCT/US2018/047095
申请日：2018-08-20
Applicant: FLUIDIGM CORPORATION
Inventor： QIN, Jian , CHEN, Peilin , RAMAKRISHNAN, Ramesh
IPC: C12N15/10 , C12Q1/6806
Abstract: Disclosed herein is a method and kit thereof for isolating nucleic acids from wax-embedded samples using hexadecane as the solvent to dissolve the sample, precipitating and washing the extracted nucleic acids using ethanol, and dissolving the nucleic acids in a lysis buffer that includes NP40 and SDS. By implementing the reagents and techniques described in this disclosure, the user can obtain a product that has better yield, less degradation, and contains more unique mRNA transcripts for subsequent sequencing and analysis.
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公开(公告)号：WO2011142836A2
公开(公告)日：2011-11-17
申请号：PCT/US2011/000887
申请日：2011-05-16
Applicant: FLUIDIGM CORPORATION , JONES, Robert, C. , LIVAK, Kenneth, J. , MAY, Andrew , MIR, Alain , PIEPRZYK, Martin , QIN, Jian , RAMAKRISHNAN, Ramesh , SPURGEON, Sandra , WANG, Jun , ZIMMERMANN, Bernhard, G.
Inventor： JONES, Robert, C. , LIVAK, Kenneth, J. , MAY, Andrew , MIR, Alain , PIEPRZYK, Martin , QIN, Jian , RAMAKRISHNAN, Ramesh , SPURGEON, Sandra , WANG, Jun , ZIMMERMANN, Bernhard, G.
CPC classification number: C12Q1/6851 , C12Q1/6855 , C12Q1/6858 , G01N33/542 , G01N33/582 , G01N33/6893 , G01N2800/368 , C12Q2521/301 , C12Q2521/501 , C12Q2525/155 , C12Q2535/131 , C12Q2537/143 , C12Q2537/16 , C12Q2563/173
Abstract: The present invention provides amplification-based methods for detection of genotype, mutations, and/or aneuploidy. These methods have broad applicability, but are particularly well-suited to detecting and quantifying target nucleic acids in free fetal DNA present in a maternal bodily fluid sample.
Abstract translation: 本发明提供用于检测基因型，突变和/或非整倍体的基于扩增的方法。 这些方法具有广泛的适用性，但是特别适合于检测和定量存在于母体体液样品中的游离胎儿DNA中的靶核酸。
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公开(公告)号：EP2569447A2
公开(公告)日：2013-03-20
申请号：EP11780930.1
申请日：2011-05-16
Applicant: Fluidigm Corporation , Pieprzyk, Martin
Inventor： PIEPRZYK, Martin , JONES, Robert C. , LIVAK, Kenneth J. , MAY, Andrew , MIR, Alain , QIN, Jian , RAMAKRISHNAN, Ramesh , SPURGEON, Sandra , WANG, Jun , ZIMMERMANN, Bernhard G.
CPC classification number: C12Q1/6851 , C12Q1/6855 , C12Q1/6858 , G01N33/542 , G01N33/582 , G01N33/6893 , G01N2800/368 , C12Q2521/301 , C12Q2521/501 , C12Q2525/155 , C12Q2535/131 , C12Q2537/143 , C12Q2537/16 , C12Q2563/173
Abstract: The present invention provides amplification-based methods for detection of genotype, mutations, and/or aneuploidy. These methods have broad applicability, but are particularly well-suited to detecting and quantifying target nucleic acids in free fetal DNA present in a maternal bodily fluid sample.
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公开(公告)号：EP3668978A1
公开(公告)日：2020-06-24
申请号：EP18845944.0
申请日：2018-08-20
Applicant: Fluidigm Corporation
Inventor： QIN, Jian , CHEN, Peilin , RAMAKRISHNAN, Ramesh
IPC: C12N15/10 , C12Q1/6806
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