公开(公告)号：WO2016026506A1

公开(公告)日：2016-02-25

申请号：PCT/EP2014/067549

申请日：2014-08-18

Applicant: FOSS ANALYTICAL A/S

Inventor： NOERGAARD, Lars ,  BAUM, Andreas ,  JUHL, Henrik, Vilstrup ,  HANSEN, Per, Waaben ,  MIKKELSEN, Joern, Dalgaard

IPC: G01N33/04 ,  G01N21/35 ,  G06F19/00 ,  G01N33/14

CPC classification number: G01N21/3577 ,  C12Q1/37 ,  G01N21/35 ,  G01N21/75 ,  G01N33/04 ,  G01N33/143 ,  G01N33/146 ,  G01N2201/127 ,  G01N2201/129 ,  G01N2333/9648 ,  G06F19/701 ,  G06F19/702 ,  G06F19/707

Abstract: A method of determining a constituent related sample property of a multi-constituent sample comprising: subjecting the sample to a perturbation selected to induce a time dependent change in measurement data associated with a constituent related to the sample property to be determined; recording a time-series of measurement data following subjecting the sample to the perturbation; and determining the sample property from the application to the recorded time-series of measurement data of a calibration correlating the sample property with time-series of measurement data, said calibration being empirically derived from chemometric time-series modelling of time-series measurement data recorded for each of a plurality of reference samples following subjecting each reference sample to the perturbation, each reference sample having a different known values of the sample property.

Abstract translation: 一种确定多组分样品的组成相关样品性质的方法，包括：使所述样品经受选择的扰动，以引起与待测定的样品性质相关的成分相关的测量数据的时间依赖性变化; 在对样本进行扰动之后记录测量数据的时间序列; 以及将应用程序的样本属性确定为将样本属性与测量数据的时间序列相关联的校准的测量数据的记录时间序列，所述校准是经验地从记录的时间序列测量数据的化学计量时间序列建模得出的 对于多个参考样本中的每一个，在对每个参考样本进行扰动之后，每个参考样本具有不同的样本特性的已知值。

公开(公告)号：WO2014032695A1

公开(公告)日：2014-03-06

申请号：PCT/EP2012/066650

申请日：2012-08-28

Applicant: FOSS ANALYTICAL A/S ,  DANMARKS TEKNISKE UNIVERSITET ,  BAUM, Andreas ,  MIKKELSEN, Jørn ,  EGEBO, Max Sass

Inventor： BAUM, Andreas ,  MIKKELSEN, Jørn ,  EGEBO, Max Sass

IPC: C12Q1/00 ,  G06F19/12

CPC classification number: C12Q1/00 ,  G06F19/702 ,  G06F19/707

Abstract: A method of determining an indication of enzymatic kinetics of a monitored enzymatic reaction comprises: originating temporal evolution data, particularly FTIR spectral fingerprint evolution data, of one or both of a substrate and a product of an enzymatic reaction for different known enzymatic activities; establishing in a data processor a calibration correlating enzymatic kinetics with temporal evolution data by applying a chemometric multi-way model to data representative of the originated temporal evolution data; originating corresponding temporal evolution data for a monitored enzymatic reaction of unknown kinetics; providing to the data processor data representing the provided temporal evolution data; and applying to that data the calibration to determine thereby in the data processor a quantitative and/or qualitative indication of enzymatic kinetics of the monitored enzymatic reaction.

Abstract translation: 确定所监测的酶促反应的酶动力学指示的方法包括：对不同已知酶活性的底物和酶反应产物之一或两者起始时间进化数据，特别是FTIR光谱指纹进化数据; 在数据处理器中建立校准，使酶动力学与时间演化数据相关联，通过将化学计量多路模型应用于代表起始时间进化数据的数据; 产生用于未知动力学的被监测的酶反应的相应的时间演变数据; 向所述数据处理器提供表示所提供的时间演进数据的数据; 并且对该数据应用该校准，从而在数据处理器中确定所监测的酶反应的酶动力学的定量和/或定性指示。

公开(公告)号：EP3183571A1

公开(公告)日：2017-06-28

申请号：EP14755632.8

申请日：2014-08-18

Applicant: FOSS Analytical A/S

Inventor： NOERGAARD, Lars ,  BAUM, Andreas ,  JUHL, Henrik, Vilstrup ,  HANSEN, Per, Waaben ,  MIKKELSEN, Joern, Dalgaard

IPC: G01N33/04 ,  G01N21/35 ,  G06F19/00 ,  G01N33/14

CPC classification number: G01N21/3577 ,  C12Q1/37 ,  G01N21/35 ,  G01N21/75 ,  G01N33/04 ,  G01N33/143 ,  G01N33/146 ,  G01N2201/127 ,  G01N2201/129 ,  G01N2333/9648 ,  G06F19/701 ,  G06F19/702 ,  G06F19/707

Abstract: A method of determining a constituent related sample property of a multi-constituent sample comprising: subjecting the sample to a perturbation selected to induce a time dependent change in measurement data associated with a constituent related to the sample property to be determined; recording a time-series of measurement data following subjecting the sample to the perturbation; and determining the sample property from the application to the recorded time-series of measurement data of a calibration correlating the sample property with time-series of measurement data, said calibration being empirically derived from chemometric time-series modelling of time-series measurement data recorded for each of a plurality of reference samples following subjecting each reference sample to the perturbation, each reference sample having a different known values of the sample property.

Abstract translation: 一种确定多组分样品的成分相关样品性质的方法，包括：使样品受到扰动，所述扰动选择为引起与待确定的样品性质相关的成分相关联的测量数据的时间依赖性变化; 在对样本进行微扰之后记录测量数据的时间序列; 以及从应用程序确定记录的时间序列的校准的测量数据的样本属性，将样本属性与测量数据的时间序列相关联，所述校准根据经验从所记录的时间序列测量数据的化学计量时间序列建模 对于每个参考样本进行扰动后的多个参考样本中的每一个，每个参考样本具有不同的样本属性的已知值。