公开(公告)号：EP1164199A4

公开(公告)日：2002-05-08

申请号：EP00908024

申请日：2000-03-13

Applicant: FUSO PHARMACEUTICAL IND

Inventor： SUGIYAMA ATSUSHI

IPC: C12Q1/06 ,  C12Q1/00 ,  C12Q1/26 ,  C12Q1/32 ,  C12Q1/34 ,  C12Q1/42 ,  C12Q1/48 ,  C12Q1/527 ,  C12Q1/533

CPC classification number: G01N33/5308 ,  C12Q1/008 ,  C12Q1/527 ,  G01N33/573

Abstract: A method for quickly assaying the cAMP content or the adenylate cyclase activity in a biological sample containing endogenous non-cyclic adenine nucleotides without resort to any radioactive reagents. More particularly, a method for assaying the cAMP content or the adenylate cyclase activity in a biological sample containing endogenous non-cyclic adenine nucleotides selected from the group consisting of cAMP formed by endogenous adenylate cyclase, ATP, AMP, ADP and mixtures thereof which comprises: (1) adding efficacious amounts of apyrase, adenosine deaminase and alkaline phosphatase to the above sample to thereby enzymatically remove the endogenous non-cyclic adenine nucleotides other than cAMP and glycose-6-phosphate therefrom; (2) enzymatically converting cAMP into AMP; and (3) thus quantitating AMP without resort to any radioactive substances; and a kit for performing this method.

公开(公告)号：CA2367904A1

公开(公告)日：2000-09-21

申请号：CA2367904

申请日：2000-03-13

Applicant: FUSO PHARMACEUTICAL IND

Inventor： SUGIYAMA ATSUSHI

IPC: C12Q1/06 ,  C12Q1/00 ,  C12Q1/26 ,  C12Q1/32 ,  C12Q1/34 ,  C12Q1/42 ,  C12Q1/48 ,  C12Q1/527 ,  C12Q1/533

Abstract: A method for quickly assaying the cAMP content or the adenylate cyclase activity in a biological sample containing endogenous non-cyclic adenine nucleotides without resort to any radioactive reagents. More particularly, a method for assaying the cAMP content or the adenylate cyclase activity in a biological sample containing endogenous non-cyclic adenine nucleotides selected from the group consisting of cAMP formed by endogenous adenylate cyclase, ATP, AMP, ADP and mixtures thereof which comprises: (1) adding efficacious amounts of apyrase, adenosine deaminase and alkaline phosphatase to the above sample to thereby enzymatically remove the endogenous non-cycli c adenine nucleotides other than cAMP and glycose-6-phosphate therefrom; (2) enzymatically converting cAMP into AMP; and (3) thus quantitating AMP withou t resort to any radioactive substances; and a kit for performing this method.

公开(公告)号：DE60028555T2

公开(公告)日：2007-05-16

申请号：DE60028555

申请日：2000-03-13

Applicant: FUSO PHARMACEUTICAL IND

Inventor： SUGIYAMA ATSUSHI

IPC: C12Q1/06 ,  C12Q1/00 ,  C12Q1/26 ,  C12Q1/32 ,  C12Q1/34 ,  C12Q1/42 ,  C12Q1/48 ,  C12Q1/527 ,  C12Q1/533

Abstract: The present invention relates to a method for quickly determining cAMP content or an adenylate cyclase activity in a biological sample containing non-cyclic adenine nucleotides without the use of radioactive agents. Particularly, the present invention provides a method of determining cAMP content or an adenylate cyclase activity in a biological sample containing non-cyclic adenine nucleotides selected from the group consisting of cAMP produced by endogenous adenylate cyclase, and AMP, ATP, ADP and a mixture thereof, which comprises (1) combining a biological sample with effective amounts of apyrase, adenosine deaminase and alkaline phosphatase to enzymatically remove non-cyclic adenine nucleotides other than cAMP, and glucose-6-phosphate in the sample; (2) enzymatically converting cAMP into AMP; (3) determining an amount of AMP without the use of radioactive agents, and a kit to carry out the method.

公开(公告)号：ES2265913T3

公开(公告)日：2007-03-01

申请号：ES00908024

申请日：2000-03-13

Applicant: FUSO PHARMACEUTICAL IND

Inventor： SUGIYAMA ATSUSHI

IPC: C12Q1/06 ,  C12Q1/00 ,  C12Q1/26 ,  C12Q1/32 ,  C12Q1/34 ,  C12Q1/42 ,  C12Q1/48 ,  C12Q1/527 ,  C12Q1/533

Abstract: Un método para eliminar los nucleótidos de adenina no cíclicos, componiéndose de ATP, ADP y AMP endógenos, y la glucosa-6-fosfato endógena en una muestra biológica, el cual comprende el tratar dicha muestra con cantidades eficaces de apirasa, fosfatasa alcalina y adenosina desaminasa, con exclusión de la 5¿-nucleotidasa, para eliminar dichos nucleótidos de adenina no cíclicos y la glucosa-6-fosfato.

公开(公告)号：AU758115B2

公开(公告)日：2003-03-13

申请号：AU2943000

申请日：2000-03-13

Applicant: FUSO PHARMACEUTICAL IND

Inventor： SUGIYAMA ATSUSHI

IPC: C12Q1/06 ,  C12Q1/00 ,  C12Q1/26 ,  C12Q1/32 ,  C12Q1/34 ,  C12Q1/42 ,  C12Q1/48 ,  C12Q1/527 ,  C12Q1/533

Abstract: The present invention relates to a method for quickly determining cAMP content or an adenylate cyclase activity in a biological sample containing non-cyclic adenine nucleotides without the use of radioactive agents. Particularly, the present invention provides a method of determining cAMP content or an adenylate cyclase activity in a biological sample containing non-cyclic adenine nucleotides selected from the group consisting of cAMP produced by endogenous adenylate cyclase, and AMP, ATP, ADP and a mixture thereof, which comprises (1) combining a biological sample with effective amounts of apyrase, adenosine deaminase and alkaline phosphatase to enzymatically remove non-cyclic adenine nucleotides other than cAMP, and glucose-6-phosphate in the sample; (2) enzymatically converting cAMP into AMP; (3) determining an amount of AMP without the use of radioactive agents, and a kit to carry out the method.

公开(公告)号：CA2367904C

公开(公告)日：2011-09-13

申请号：CA2367904

申请日：2000-03-13

Applicant: FUSO PHARMACEUTICAL IND

Inventor： SUGIYAMA ATSUSHI

IPC: C12Q1/06 ,  C12Q1/00 ,  C12Q1/26 ,  C12Q1/32 ,  C12Q1/34 ,  C12Q1/42 ,  C12Q1/48 ,  C12Q1/527 ,  C12Q1/533

Abstract: The present invention relates to a method for quickly determining cAMP content or an adenylate cyclase activity in a biological sample containing non-cyclic adenine nucleotides without the use of radioactive agents. Particularly, the present invention provides a method of determining cAMP content or an adenylate cyclase activity in a biological sample containing non-cyclic adenine nucleotides selected from the group consisting of cAMP produced by endogenous adenylate cyclase, and AMP, ATP, ADP and a mixture thereof, which comprises (1) combining a biological sample with effective amounts of apyrase, adenosine deaminase and alkaline phosphatase to enzymatically remove non-cyclic adenine nucleotides other than cAMP, and glucose-6-phosphate in the sample; (2) enzymatically converting cAMP into AMP; (3) determining an amount of AMP without the use of radioactive agents, and a kit to carry out the method.

公开(公告)号：DE60028555D1

公开(公告)日：2006-07-20

申请号：DE60028555

申请日：2000-03-13

Applicant: FUSO PHARMACEUTICAL IND

Inventor： SUGIYAMA ATSUSHI

IPC: C12Q1/06 ,  C12Q1/00 ,  C12Q1/26 ,  C12Q1/32 ,  C12Q1/34 ,  C12Q1/42 ,  C12Q1/48 ,  C12Q1/527 ,  C12Q1/533

Abstract: The present invention relates to a method for quickly determining cAMP content or an adenylate cyclase activity in a biological sample containing non-cyclic adenine nucleotides without the use of radioactive agents. Particularly, the present invention provides a method of determining cAMP content or an adenylate cyclase activity in a biological sample containing non-cyclic adenine nucleotides selected from the group consisting of cAMP produced by endogenous adenylate cyclase, and AMP, ATP, ADP and a mixture thereof, which comprises (1) combining a biological sample with effective amounts of apyrase, adenosine deaminase and alkaline phosphatase to enzymatically remove non-cyclic adenine nucleotides other than cAMP, and glucose-6-phosphate in the sample; (2) enzymatically converting cAMP into AMP; (3) determining an amount of AMP without the use of radioactive agents, and a kit to carry out the method.

公开(公告)号：AT329052T

公开(公告)日：2006-06-15

申请号：AT00908024

申请日：2000-03-13

Applicant: FUSO PHARMACEUTICAL IND

Inventor： SUGIYAMA ATSUSHI

IPC: C12Q1/06 ,  C12Q1/00 ,  C12Q1/26 ,  C12Q1/32 ,  C12Q1/34 ,  C12Q1/42 ,  C12Q1/48 ,  C12Q1/527 ,  C12Q1/533

Abstract: The present invention relates to a method for quickly determining cAMP content or an adenylate cyclase activity in a biological sample containing non-cyclic adenine nucleotides without the use of radioactive agents. Particularly, the present invention provides a method of determining cAMP content or an adenylate cyclase activity in a biological sample containing non-cyclic adenine nucleotides selected from the group consisting of cAMP produced by endogenous adenylate cyclase, and AMP, ATP, ADP and a mixture thereof, which comprises (1) combining a biological sample with effective amounts of apyrase, adenosine deaminase and alkaline phosphatase to enzymatically remove non-cyclic adenine nucleotides other than cAMP, and glucose-6-phosphate in the sample; (2) enzymatically converting cAMP into AMP; (3) determining an amount of AMP without the use of radioactive agents, and a kit to carry out the method.

公开(公告)号：DK1164199T3

公开(公告)日：2006-07-10

申请号：DK00908024

申请日：2000-03-13

Applicant: FUSO PHARMACEUTICAL IND

Inventor： SUGIYAMA ATSUSHI

IPC: C12Q1/06 ,  C12Q1/00 ,  C12Q1/26 ,  C12Q1/32 ,  C12Q1/34 ,  C12Q1/42 ,  C12Q1/48 ,  C12Q1/527 ,  C12Q1/533

Abstract: The present invention relates to a method for quickly determining cAMP content or an adenylate cyclase activity in a biological sample containing non-cyclic adenine nucleotides without the use of radioactive agents. Particularly, the present invention provides a method of determining cAMP content or an adenylate cyclase activity in a biological sample containing non-cyclic adenine nucleotides selected from the group consisting of cAMP produced by endogenous adenylate cyclase, and AMP, ATP, ADP and a mixture thereof, which comprises (1) combining a biological sample with effective amounts of apyrase, adenosine deaminase and alkaline phosphatase to enzymatically remove non-cyclic adenine nucleotides other than cAMP, and glucose-6-phosphate in the sample; (2) enzymatically converting cAMP into AMP; (3) determining an amount of AMP without the use of radioactive agents, and a kit to carry out the method.

公开(公告)号：JP2000262296A

公开(公告)日：2000-09-26

申请号：JP7369099

申请日：1999-03-18

Applicant: FUSO PHARMACEUTICAL IND

Inventor： SUGIYAMA ATSUSHI

IPC: C12Q1/06 ,  C12Q1/00 ,  C12Q1/26 ,  C12Q1/32 ,  C12Q1/34 ,  C12Q1/42 ,  C12Q1/48 ,  C12Q1/527 ,  C12Q1/533

Abstract: PROBLEM TO BE SOLVED: To measure cAMP without using a radioactive reagent by treating a biological sample with apyrase, alkali phosphatase, adenosine deaminase, enzymatically converting cAMP to AMP and detecting the produced AMP. SOLUTION: In this method for measuring cAMP amount or adenylate cyclase activity in a biological sample, the biological sample is treated with apyrase, alkaliphosphatase and adenosine deaminase in an amount which is effective for removing noncyclic adenine nucleotides comprising endogenic ATP, ADP and AMP, and glucose-6-phosphoric acid and cAMP in the biological sample is. enzymatically converted to AMP and then, the produced AMP amount is measured without using a radioactive reagent to rapidly measure adenylate cyclase activity capable of measuring cAMP mediating hyperglycemic action of adrenaline and glucagon in liver and change of an amount of the cAMP.