中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

15 records (took 0.029 seconds)

    METHODS OF PRODUCING PROTEINS HAVING TRIPLE-HELIX STRUCTURE
    1.
    发明公开
    METHODS OF PRODUCING PROTEINS HAVING TRIPLE-HELIX STRUCTURE 有权
    用于生产蛋白的三螺旋

    公开(公告)号：EP1870460A4

    公开(公告)日：2008-12-24

    申请号：EP06730889

    申请日：2006-03-31

    Applicant: FUSO PHARMACEUTICAL IND ,  OSAKA PREFECTURE

    Inventor： KASE TETSUO ,  KIMURA AKIO ,  KISAKI HIROSHI ,  KESHI HIROYUKI ,  UEYAMA HIROSHI ,  NISHIHARA MIZUKI

    IPC: C12N15/09 ,  C07K14/78 ,  C12N5/10 ,  C12P21/02

    CPC classification number: C12P21/02 ,  C07K14/78

    Abstract: Disclosed is a process for producing a human collagen molecule having substantially the same structure as that of a naturally occurring collagen molecule, which process can synthesize, in a host cell having a collagen gene introduced therein, a large amount of a human collagen protein derived from the gene introduced in a vector capable of expressing a foreign gene at a high level, and which process enables to isolate and purify the human collagen molecule in a simple manner. Also disclosed is a collagen molecule produced by the process. A collagen gene construct is introduced into a vector capable of expressing a foreign gene at a high level, and a cell capable of expressing a collagen molecule at a low level is selected from a variety of mammalian cells as a host, whereby human collagen with little contamination by host cell-derived collagen can be produced in a large amount.

    METHODS OF PRODUCING PROTEINS HAVING TRIPLE-HELIX STRUCTURE
    4.
    发明专利
    METHODS OF PRODUCING PROTEINS HAVING TRIPLE-HELIX STRUCTURE 未知

    公开(公告)号：CA2602611C

    公开(公告)日：2015-09-29

    申请号：CA2602611

    申请日：2006-03-31

    Applicant: FUSO PHARMACEUTICAL IND ,  OSAKA PREFECTURE

    Inventor： KASE TETSUO ,  KIMURA AKIO ,  KISAKI HIROSHI ,  KESHI HIROYUKI ,  UEYAMA HIROSHI ,  NISHIHARA MIZUKI

    IPC: C12N15/09 ,  C07K14/78 ,  C12N5/10 ,  C12P21/02

    Abstract: An objective of the present invention is to provide methods of producing human collagen molecules that are easy to isolate and purify and that have a structure substantially equivalent to that of a natural collagen molecule, wherein host cells that are transduced with a collagen gene synthesize large amounts of human collagen protein derived from a gene introduced into a high exogenous gene expression vector. Another objective of the present invention is to provide collagen molecules produced by the production methods. The present inventors discovered that a large amount of human collagen hardly contaminated with host cell-derived collagen could be produced, by selecting from various mammalian cells a host cell that has low collagen expression and introducing a collagen gene construct into a vector capable of high exogenous gene expression.

    ">
    6.
    发明专利
    "Process for production of protein having triple-helical structure" 未知

    公开(公告)号：AU2012200573A1

    公开(公告)日：2012-02-23

    申请号：AU2012200573

    申请日：2012-02-01

    Applicant: FUSO PHARMACEUTICAL IND ,  OSAKA PREFECTURE

    Inventor： KASE TETSUO ,  KIMURA AKIO ,  KISAKI HIROSHI ,  KESHI HIROYUKI ,  UEYAMA HIROSHI ,  NISHIHARA MIZUKI

    IPC: C12N15/09 ,  C07K14/78 ,  C12N5/10 ,  C12P21/02

    Abstract: An objective of the present invention is to provide methods of producing human collagen molecules that are easy to isolate and purify and that have a structure substantially equivalent to that of a natural collagen molecule, wherein host cells that are transduced with a collagen gene synthesize large amounts of human collagen protein derived from a gene introduced into a high exogenous gene expression vector. Another objective of the present invention is to provide collagen molecules produced by the production methods. The present inventors discovered that a large amount of human collagen hardly contaminated with host cell-derived collagen could be produced, by selecting from various mammalian cells a host cell that has low collagen expression and introducing a collagen gene construct into a vlector capable of high exogenous gene expression.

    Process for production of protein having triple-helical structure
    7.
    发明专利
    Process for production of protein having triple-helical structure 未知

    公开(公告)号：AU2006231795B2

    公开(公告)日：2012-02-16

    申请号：AU2006231795

    申请日：2006-03-31

    Applicant: OSAKA PREFECTURE ,  FUSO PHARMACEUTICAL IND

    Inventor： KASE TETSUO ,  KISAGI HIROSHI ,  NISHIHARA MIZUKI ,  CHASHI HIROYUKI ,  KIMURA AKIO ,  KAMIYAMA HIROSHI

    IPC: C12N15/09 ,  C07K14/78 ,  C12N5/10 ,  C12P21/02

    Abstract: Disclosed is a process for producing a human collagen molecule having substantially the same structure as that of a naturally occurring collagen molecule, which process can synthesize, in a host cell having a collagen gene introduced therein, a large amount of a human collagen protein derived from the gene introduced in a vector capable of expressing a foreign gene at a high level, and which process enables to isolate and purify the human collagen molecule in a simple manner. Also disclosed is a collagen molecule produced by the process. A collagen gene construct is introduced into a vector capable of expressing a foreign gene at a high level, and a cell capable of expressing a collagen molecule at a low level is selected from a variety of mammalian cells as a host, whereby human collagen with little contamination by host cell-derived collagen can be produced in a large amount.

    Process for production of protein having triple-helical structure
    8.
    发明专利
    Process for production of protein having triple-helical structure 未知

    公开(公告)号：AU2006231795A1

    公开(公告)日：2006-10-12

    申请号：AU2006231795

    申请日：2006-03-31

    Applicant: OSAKA PREFECTURE ,  FUSO PHARMACEUTICAL IND

    Inventor： KASE TETSUO ,  KISAGI HIROSHI ,  NISHIHARA MIZUKI ,  CHASHI HIROYUKI ,  KIMURA AKIO ,  KAMIYAMA HIROSHI

    IPC: C12N15/09 ,  C07K14/78 ,  C12N5/10 ,  C12P21/02

    Abstract: An objective of the present invention is to provide methods of producing human collagen molecules that are easy to isolate and purify and that have a structure substantially equivalent to that of a natural collagen molecule, wherein host cells that are transduced with a collagen gene synthesize large amounts of human collagen protein derived from a gene introduced into a high exogenous gene expression vector. Another objective of the present invention is to provide collagen molecules produced by the production methods. The present inventors discovered that a large amount of human collagen hardly contaminated with host cell-derived collagen could be produced, by selecting from various mammalian cells a host cell that has low collagen expression and introducing a collagen gene construct into a vector capable of high exogenous gene expression.

    PROCESS FOR PRODUCTION OF PROTEIN HAVING TRIPLE-HELICAL STRUCTURE
    9.
    发明专利
    PROCESS FOR PRODUCTION OF PROTEIN HAVING TRIPLE-HELICAL STRUCTURE 未知

    公开(公告)号：CA2602611A1

    公开(公告)日：2006-10-12

    申请号：CA2602611

    申请日：2006-03-31

    Applicant: FUSO PHARMACEUTICAL IND ,  OSAKA PREFECTURE

    Inventor： NISHIHARA MIZUKI ,  KISAKI HIROSHI ,  KIMURA AKIO ,  KESHI HIROYUKI ,  KASE TETSUO ,  UEYAMA HIROSHI

    IPC: C12N15/09 ,  C07K14/78 ,  C12N5/10 ,  C12P21/02

    Abstract: Disclosed is a process for producing a human collagen molecule having substantially the same structure as that of a naturally occurring collagen molecule, which process can synthesize, in a host cell having a collagen gen e introduced therein, a large amount of a human collagen protein derived from the gene introduced in a vector capable of expressing a foreign gene at a hi gh level, and which process enables to isolate and purify the human collagen molecule in a simple manner. Also disclosed is a collagen molecule produced by the process. A collagen gene construct is introduced into a vector capable o f expressing a foreign gene at a high level, and a cell capable of expressing a collagen molecule at a low level is selected from a variety of mammalian cel ls as a host, whereby human collagen with little contamination by host cell- derived collagen can be produced in a large amount.

    Procedimiento para la produccíón de colágeno humano de tipo II
    10.
    发明专利
    Procedimiento para la produccíón de colágeno humano de tipo II 未知

    公开(公告)号：ES2489715T3

    公开(公告)日：2014-09-02

    申请号：ES11002048

    申请日：2006-03-31

    Applicant: FUSO PHARMACEUTICAL IND ,  OSAKA PREFECTURE

    Inventor： KASE TETSUO ,  KIMURA AKIO ,  UEYAMA HIROSHI ,  NISHIHARA MIZUKI ,  KISAKI HIROSHI ,  KESHI HIROYUKI

    IPC: C12N15/09 ,  C07K14/78 ,  C12N5/10 ,  C12P21/02

    Abstract: Un método de producción de una proteina que tiene una estructura de triple helice, en el que el método comprende: (a) introducir ADN que codifica una proteina que tiene una estructura de triple helice en el vector pNOW/CMV-AA, en el que el ADN se selecciona de: (i) un ADN que comprende la secuencia de nucleetidos de SEQ ID NO: 7; y (ii) un ADN que se hibrida en condiciones rigurosas con un ADN que comprende la secuencia de nucleetidos de SEQ ID NO: 7; (b) transformar una celula CHO por transferencia del vector genico; y (c) cultivar o reproducir el transformante, y recoger la proteina que tiene una estructura de triple helice de la celula o del sobrenadante del cultivo de la misma, en el que la proteina que tiene una estructura de triple helice es colageno humano de tipo II o un peptido parcial del mismo.

Patent Agency Ranking