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公开(公告)号：US20220011558A1
公开(公告)日：2022-01-13
申请号：US17482678
申请日：2021-09-23
Applicant: Femtonics Kft.
Inventor： Balazs ROZSA , Gergely KATONA , Pal MAAK , Mate VERESS , Andras FEHER , Gergely SZALAY , Peter MATYAS
Abstract: The invention relates to a method for correcting motion artifacts of in vivo fluorescence measurements using a 3D laser scanning microscope containing two pairs of orthogonally arranged acousto-optic deflectors, the method comprising: selecting a region of interest, selecting a plurality of guiding points along the region of interest, extending the guiding points to objects selected from scanning lines and/or surface elements and/or volume elements which, together, substantially cover the region of interest, repeatedly scanning the objects by generating continuous drifts to cover the objects, projecting the obtained scanning data to frames and obtaining a time series of the frames, correcting motion artifacts by shifting the data of the successive frames with respect to each other so as to maximize fluorescence cross correlation between the data of the frames.
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公开(公告)号：US20190285865A1
公开(公告)日：2019-09-19
申请号：US16290238
申请日：2019-03-01
Applicant: Femtonics Kft.
Inventor： Balazs ROZSA , Gergely KATONA , Pal MAAK , Mate VERESS , Andras FEHER , Gergely SZALAY , Peter MATYAS
Abstract: The invention relates to a method for scanning along a substantially straight line (3D line) lying at an arbitrary direction in a 3D space with a given speed using a 3D laser scanning microscope having a first pair of acousto-optic deflectors deflecting a laser beam in the x-z plane (x axis deflectors) and a second pair of acousto-optic deflectors deflecting the laser beam in the y-z plane (y axis deflectors) for focusing the laser beam in 3D.The invention further relates to a method for scanning a region of interest with a 3D laser scanning microscope having acousto-optic deflectors for focusing a laser beam within a 3D space defined by an optical axis (Z) of the microscope and X, Y axes that are perpendicular to the optical axis and to each other
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