公开(公告)号：US12000838B2

公开(公告)日：2024-06-04

申请号：US17050716

申请日：2019-04-26

Applicant: Fluidigm Canada Inc.

Inventor： Alexander V. Loboda ,  Olga Ornatsky ,  Vladimir Baranov ,  Nikita Zabinyakov ,  Anastasia Mavropoulos ,  Daniel Majonis ,  Bedilu Allo ,  Taunia Closson

IPC: G01N33/68 ,  C07K1/13 ,  G01N1/28

CPC classification number: G01N33/6848 ,  C07K1/13 ,  G01N1/28 ,  G01N2458/15 ,  G01N2560/00

Abstract: Embodiments of the present invention relate to reagents and their use for elemental imaging mass spectrometry of biological samples. The embodiments comprising methods for quantifying one or more analytes within a sample, comprising the steps of: (a) providing the sample, wherein the one or more analytes are immobilized to a sample carrier, wherein the sample has been labelled with one or more mass tags comprising one or more labelling atoms, (b) performing mass cytometry on the sample to determine the level of the one or more labelling atoms, wherein the level of the one or more labelling atoms corresponds to the copy number of the one or more analytes.

公开(公告)号：US20220288246A1

公开(公告)日：2022-09-15

申请号：US17632438

申请日：2020-08-07

Applicant: Fluidigm Canada Inc. ,  THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTO

Inventor： Peng Liu ,  Daniel Majonis ,  Vladimir Baranov ,  Mitchel A. Winnik ,  Hyungjun Cho

IPC: A61K51/10 ,  A61K51/06

Abstract: A new class of mass-tag polymers is provided, which include enriched metal isotopes such as zirconium and hafnium mass tags. The chemistry of these new mass tags are different from that of lanthanide mass tags, and opens up new mass channels that can be used in mass cytometry. These polymers may be used for mass cytometry, therapeutic delivery of a radioactive isotope, or screening of a therapeutic isotope. Aspects include a kit, method of making, and method of using a polymer, isotopic composition, or both. A kit may include a polymer. The polymer may include pendant groups that chelate an enriched isotope, such as zirconium and/or hafnium. The kit may include an isotopic composition comprising an enriched zirconium or hafnium isotope. Polymers may be conjugated to a biologically active material. Aspects may also include making a kit. Aspects include use of a kit, such as for mass cytometry.

公开(公告)号：US20220155308A1

公开(公告)日：2022-05-19

申请号：US17439503

申请日：2020-03-27

Applicant: Fluidigm Canada Inc.

Inventor： Stephen K.H. Li ,  Daniel Majonis ,  Vladimir Baranov ,  Olga Ornatsky ,  Bedilu Allo ,  Dmitry Bandura ,  Colin Thom ,  Greg Stelzer ,  Alexander Loboda

IPC: G01N33/58 ,  G01N1/30 ,  G01N15/10

Abstract: A lyophilized antibody panel is disclosed for interrogation using elemental analysis. The antibody panel includes multiple antibodies each element-tagged or element-labelled with one or more isotopes such that each different antibody is isotopically distinguishable from the other antibodies. Each element tag can include one or more unique isotopes or unique combinations of isotopes. The set of element-tagged antibodies can be lyophilized in admixture. Thus, the lyophilized element-tagged antibody panel can be easily and efficiently resuspended and mixed with a sample prior to interrogation with an elemental analyzer, such as a mass spectrometer. This lyophilized element-tagged antibody panel can provide the benefits of an element-tagged assay while also being easy to use and remaining stable for long durations.

公开(公告)号：US20210239707A1

公开(公告)日：2021-08-05

申请号：US17050716

申请日：2019-04-26

Applicant: Fluidigm Canada Inc.

Inventor： Alexander V. Loboda ,  Olga Ornatsky ,  Vladimir Baranov ,  Nikita Zabinyakov ,  Anastasia Mavropoulos ,  Daniel Majonis ,  Bedilu Allo ,  Taunia Closson

IPC: G01N33/68 ,  G01N1/28 ,  C07K1/13

Abstract: Embodiments of the present invention relate to reagents and their use for elemental imaging mass spectrometry of biological samples. The embodiments comprising methods for quantifying one or more analytes within a sample, comprising the steps of: (a) providing the sample, wherein the one or more analytes are immobilized to a sample carrier, wherein the sample has been labelled with one or more mass tags comprising one or more labelling atoms, (b) performing mass cytometry on the sample to determine the level of the one or more labelling atoms, wherein the level of the one or more labelling atoms corresponds to the copy number of the one or more analytes.