公开(公告)号：AU2019247650A1

公开(公告)日：2020-10-29

申请号：AU2019247650

申请日：2019-04-02

Applicant: HARVARD COLLEGE

Inventor： NOVAK RICHARD ,  JALILI-FIROOZINEZHAD SASAN ,  GAZZANIGA FRANCESCA S ,  CALAMARI ELIZABETH L ,  CAMACHO DIOGO M ,  NESTOR BRET A ,  FADEL CICELY ,  CRONCE MICHAEL L ,  KASPER DENNIS L ,  INGBER DONALD E ,  BEIN AMIR

IPC: C12M1/12 ,  C12M3/06 ,  G01N33/48 ,  G01N33/50

Abstract: A microfluidic device is directed to sustaining a complex microbial community in direct and indirect contact with living human intestinal cells in vitro. The device includes a first microchannel having cultured cells of a human intestinal epithelium and microbiota, the first microchannel further having a first level of oxygen. The device further includes a second microchannel having cultured cells of a vascular endothelium, the second microchannel further having a second level of oxygen. The device also includes a membrane located at an interface region between the first microchannel and the second microchannel, the membrane being composed of an oxygen-permeable material or further having pores via which oxygen flows between the first microchannel and the second microchannel to form a physiologically-relevant oxygen gradient.

公开(公告)号：CA3095749A1

公开(公告)日：2019-10-10

申请号：CA3095749

申请日：2019-04-02

Applicant: HARVARD COLLEGE

Inventor： NOVAK RICHARD ,  JALILI-FIROOZINEZHAD SASAN ,  GAZZANIGA FRANCESCA S ,  CALAMARI ELIZABETH L ,  CAMACHO DIOGO M ,  NESTOR BRET A ,  FADEL CICELY ,  CRONCE MICHAEL L ,  KASPER DENNIS L ,  INGBER DONALD E ,  BEIN AMIR

IPC: C12M1/12 ,  C12M3/06 ,  G01N33/48 ,  G01N33/50

Abstract: A microfluidic device is directed to sustaining a complex microbial community in direct and indirect contact with living human intestinal cells in vitro. The device includes a first microchannel having cultured cells of a human intestinal epithelium and microbiota, the first microchannel further having a first level of oxygen. The device further includes a second microchannel having cultured cells of a vascular endothelium, the second microchannel further having a second level of oxygen. The device also includes a membrane located at an interface region between the first microchannel and the second microchannel, the membrane being composed of an oxygen-permeable material or further having pores via which oxygen flows between the first microchannel and the second microchannel to form a physiologically-relevant oxygen gradient.