公开(公告)号：AU2007309701A1

公开(公告)日：2008-05-02

申请号：AU2007309701

申请日：2007-02-05

Applicant: HARVARD COLLEGE

Inventor： FEIGEL ALEXANDER ,  SHEVKOPLYAS SERGEY S ,  PARKER KEVIN KIT ,  WHITESIDES GEORGE M ,  FEINBERG ADAM W

IPC: C12N5/071 ,  C12N5/077

公开(公告)号：CA2641446C

公开(公告)日：2016-06-07

申请号：CA2641446

申请日：2007-02-05

Applicant: HARVARD COLLEGE

Inventor： PARKER KEVIN KIT ,  FEINBERG ADAM W ,  WHITESIDES GEORGE M ,  SHEVKOPLYAS SERGEY S ,  FEIGEL ALEXANDER

IPC: C12N11/02 ,  A61L27/38 ,  C12N5/07 ,  C12N5/071 ,  C12N5/077 ,  C12Q1/02

Abstract: A free-standing thin film is fabricated from a structure comprising a base layer coated with a sacrificial polymer layer, which is in turn coated with a flexible polymer layer. Cells are then seeded onto the flexible polymer layer and cultured to form a tissue. The flexible polymer layer is then released from the base layer to produce a free-standing thin film comprising the tissue on the flexible polymer layer. In one embodiment, the cells are myocytes, which can be actuated to propel or displace the free-standing film. In another embodiment, the free-standing film is used to treat injured human tissue.

公开(公告)号：CA2641446A1

公开(公告)日：2008-05-02

申请号：CA2641446

申请日：2007-02-05

Applicant: HARVARD COLLEGE

Inventor： PARKER KEVIN KIT ,  SHEVKOPLYAS SERGEY S ,  FEINBERG ADAM W ,  WHITESIDES GEORGE M ,  FEIGEL ALEXANDER

IPC: C12N5/071 ,  C12N5/077

公开(公告)号：AU2007309701B2

公开(公告)日：2012-09-27

申请号：AU2007309701

申请日：2007-02-05

Applicant: HARVARD COLLEGE

Inventor： FEIGEL ALEXANDER ,  FEINBERG ADAM W ,  WHITESIDES GEORGE M ,  PARKER KEVIN KIT ,  SHEVKOPLYAS SERGEY S

IPC: C12N5/077 ,  C12N5/071

Abstract: A free-standing thin film is fabricated from a structure comprising a base layer coated with a sacrificial polymer layer, which is in turn coated with a flexible polymer layer. Cells are then seeded onto the flexible polymer layer and cultured to form a tissue. The flexible polymer layer is then released from the base layer to produce a free-standing thin film comprising the tissue on the flexible polymer layer. In one embodiment, the cells are myocytes, which can be actuated to propel or displace the free-standing film. In another embodiment, the free-standing film is used to treat injured human tissue.

公开(公告)号：CA2790873A1

公开(公告)日：2011-08-25

申请号：CA2790873

申请日：2011-02-08

Applicant: HARVARD COLLEGE

Inventor： PARKER KEVIN KIT ,  FEINBERG ADAM W ,  CHIN LAUREN E M

IPC: A61F2/02 ,  C12Q1/02

Abstract: The present invention provides methods for generating relevant in vitro models of engineered innervated tissue, as well as uses of such tissues.

公开(公告)号：WO2010011407A2

公开(公告)日：2010-01-28

申请号：PCT/US2009045001

申请日：2009-05-22

Applicant: HARVARD COLLEGE ,  KUO PO-LING ,  FEINBERG ADAM W ,  PARKER KEVIN KIT

Inventor： KUO PO-LING ,  FEINBERG ADAM W ,  PARKER KEVIN KIT

IPC: C12Q1/00

CPC classification number: G01N33/5026 ,  C12N5/0068 ,  C12N2533/30 ,  C12N2535/10 ,  G01N33/5029 ,  G01N33/5061 ,  G01N33/5082 ,  G01N2333/78

Abstract: The present invention provides methods of generating and devices of patterned soft substrates, on which cells may be seeded, as well as methods of using these substrates. Devices containing these patterned soft substrates are also provided.

Abstract translation: 本发明提供了生成可以接种细胞的图案化软基质的方法以及使用这些底物的方法。 还提供了包含这些图案化软基板的装置。

公开(公告)号：EP1991668A4

公开(公告)日：2011-06-15

申请号：EP07861227

申请日：2007-02-05

Applicant: HARVARD COLLEGE

Inventor： PARKER KEVIN KIT ,  FEINBERG ADAM W ,  WHITESIDES GEORGE M ,  SHEVKOPLYAS SERGEY S ,  FEIGEL ALEXANDER

IPC: C12N5/071 ,  C12N5/077

CPC classification number: G01N33/5061 ,  C12N5/0062 ,  C12N5/0657 ,  C12N2533/30 ,  C12N2533/52 ,  C12N2539/10 ,  G01N2500/10

公开(公告)号：EP2538875A4

公开(公告)日：2013-08-21

申请号：EP11745047

申请日：2011-02-08

Applicant: HARVARD COLLEGE

Inventor： PARKER KEVIN KIT ,  FEINBERG ADAM W ,  CHIN LAUREN E M

IPC: A61F2/02 ,  C12Q1/02

CPC classification number: G01N33/5082 ,  G01N2500/00

公开(公告)号：WO2010042856A3

公开(公告)日：2010-07-22

申请号：PCT/US2009060224

申请日：2009-10-09

Applicant: GEN HOSPITAL CORP ,  HARVARD COLLEGE ,  CHIEN KENNETH R ,  DOMIAN IBRAHIM J ,  CHIRAVURI MURALI ,  VAN DER MEER PETER ,  PARKER KEVIN KIT ,  FEINBERG ADAM W

Inventor： CHIEN KENNETH R ,  DOMIAN IBRAHIM J ,  CHIRAVURI MURALI ,  VAN DER MEER PETER ,  PARKER KEVIN KIT ,  FEINBERG ADAM W

IPC: A61L27/38 ,  A61F2/08 ,  A61L27/14 ,  A61L27/24 ,  A61L27/54

CPC classification number: A61L27/3804 ,  A61L27/507 ,  A61L27/54 ,  A61L2300/252 ,  A61L2300/258 ,  A61L2300/414 ,  A61L2300/426 ,  A61L2300/43 ,  A61L2300/432

Abstract: The present invention generally relates to a population of committed ventricular progenitor (CVP) cells and their use to generate a tissue engineered myocardium, in particular two dimensional tissue engineered myocardium which is comparable to functional ventricular heart muscle. One embodiment of present invention provides a composition and methods for the production of a tissue engineered myocardium which has functional properties of cardiac muscle, such as contractibility (e.g. contraction force) and numerous properties of mature fully functional ventricular heart muscle tissue. In particular, in one embodiment, a composition comprising the tissue engineered myocardium comprises committed ventricular progenitor (CVP) cells seeded on a free-standing biopolymer structure to form functional ventricular myocardium tissue.

Abstract translation: 本发明一般涉及定向心室祖细胞（CVP）细胞群及其用于产生组织工程化心肌的用途，特别是与功能性心室肌相当的二维组织工程心肌。 本发明的一个实施方案提供了用于生产具有心肌功能特性（例如收缩力（例如收缩力））和成熟全功能心室肌组织的众多特性的组织工程心肌的组合物和方法。 特别地，在一个实施方案中，包含组织工程化心肌的组合物包含种植于自立式生物聚合物结构上的定向心室祖细胞（CVP）细胞以形成功能性心室心肌组织。

公开(公告)号：WO2009085067A3

公开(公告)日：2009-10-01

申请号：PCT/US2008011173

申请日：2008-09-26

Applicant: HARVARD COLLEGE ,  FEINBERG ADAM W ,  PARKER KEVIN KIT ,  KUO PO-LING ,  GUO CHIN-LIN

Inventor： FEINBERG ADAM W ,  PARKER KEVIN KIT ,  KUO PO-LING ,  GUO CHIN-LIN

IPC: C12N5/02 ,  C12N5/00 ,  C12N5/077

CPC classification number: C12N5/0658 ,  C12N5/0657 ,  C12N2533/52 ,  C12N2535/10 ,  C12N2539/10

Abstract: The present invention relates to the arrangement of one or more cells in a medium or on a substrate through the use of boundary conditions, which are changes in local environment compared to the medium or substrate alone or cause an alteration of cell response upon interaction of a cell with the boundary condition.

Abstract translation: 本发明涉及通过使用边界条件在介质中或衬底上布置一个或多个细胞，所述边界条件是与单独的介质或衬底相比在局部环境中的变化或者在单独的介质或衬底上引起细胞响应的改变 细胞与边界条件。