公开(公告)号：JPH04290896A

公开(公告)日：1992-10-15

申请号：JP35082191

申请日：1991-12-11

Applicant: HOECHST AG

Inventor： ENGELS JOACHIM ,  HERRLEIN MATHIAS ,  KONRAD RENATE ,  MAG MATTHIAS

IPC: C07H21/02 ,  A61K31/7052 ,  A61K31/7088 ,  C07H19/20 ,  C07H21/00 ,  C07H21/04 ,  C12Q1/68 ,  C12Q1/6813 ,  C12Q1/6816 ,  G01N33/52 ,  G01N33/58

公开(公告)号：IE75726B1

公开(公告)日：1997-09-24

申请号：IE430191

申请日：1991-12-10

Applicant: HOECHST AG

Inventor： ENGELS JOACHIM ,  HERRLEIN MATHIAS ,  KONRAD RENATE ,  MAG MATTHIAS

IPC: C07H21/02 ,  A61K31/7052 ,  A61K31/7088 ,  C07H19/20 ,  C07H21/00 ,  C07H21/04 ,  C12Q1/68 ,  C12Q1/6813 ,  C12Q1/6816 ,  G01N33/52 ,  G01N33/58 ,  C07H19/06 ,  C07H19/16 ,  C12N15/09

Abstract: The OH group located in the 3' and/or 2' position of a nucleoside, nucleotide or oligonucleotide is derivatised to an amino or thiol group and subsequently a fluorescent dye is coupled thereto. The resulting 3'- and/or 2'-amino- and thiol-modified nucleosides, nucleotides and oligonucleotides can then be used for the synthesis of items in the presence of a template strand or of oligonucleotides, and for the detection of genetic material. They offer the advantage that the fluorescent label is no longer attached to the 5' end of the oligonucleotide or to the nucleobase and thus does not have to be introduced during the chemical synthesis as in labelling techniques hitherto disclosed, while the known and conventional methods have the disadvantage that only some polymerases can be employed for the synthesis, the acceptance of the triphosphates by the polymerases falls and, in addition, a large substrate excess is necessary.

公开(公告)号：NZ264894A

公开(公告)日：1997-07-27

申请号：NZ26489494

申请日：1994-11-10

Applicant: HOECHST AG

Inventor： PEYMAN ANUSCHIRWAN ,  UHLMANN EUGEN ,  MAG MATTHIAS ,  KRETZSCHMAR GERHARD ,  HELSBERG MATTHIAS ,  WINKLER IRVIN

IPC: C12N15/09 ,  A61K31/70 ,  A61K31/7088 ,  A61P31/12 ,  A61P31/22 ,  A61P35/00 ,  C07H21/00 ,  C07H21/04

Abstract: Oligonucleotides (I) having at least one non-terminal pyrimidine nucleoside modified and additionally having the 5'- and/or 3'-terminal modified are new, with some specific exclusions. Oligonucleotides (I) having at least one non-terminal pyrimidine nucleoside modified and additionally having the 5'- and/or 3'-terminal modified are new, with the exception of oligonucleotides in which all of the nucleosides are modified and Cys asterisk -Ala-Cys-Cys-Ala asterisk -Ala-Cys-Cys-Thr asterisk -Cys-Thr-Thr-Cys asterisk -Cys-Ala-Cys-Ala-, Cys asterisk -Ala-Cys asterisk -Cys-Ala asterisk -Ala-Cys asterisk -Thr-Thr-Cys-Thr asterisk -Thr-Cys asterisk -Cys-Ala asterisk -Cys-Ala-, Cys-Ala asterisk -Cys-Cys asterisk -Ala-Ala asterisk -Cys-Thr asterisk -Thr-Cys asterisk -Thr-Thr asterisk -Cys-Cys asterisk -Ala-Cys asterisk -Ala- and Cys-Ala-Cys asterisk -Cys asterisk -Ala-Ala-Cys asterisk -Thr asterisk -Thr-Cys-Thr asterisk -Thr asterisk -Thr-Cys-Cys-Ala asterisk -Cys asterisk -Ala. An Independent claim is included for the preparation of (I) by chemical synthesis.

公开(公告)号：DK0490281T3

公开(公告)日：1997-02-10

申请号：DK91120935

申请日：1991-12-06

Applicant: HOECHST AG

Inventor： ENGELS JOACHIM ,  HERRLEIN MATHIAS ,  KONRAD RENATE ,  MAG MATTHIAS

IPC: C07H21/02 ,  A61K31/7052 ,  A61K31/7088 ,  C07H19/20 ,  C07H21/00 ,  C07H21/04 ,  C12Q1/68 ,  C12Q1/6813 ,  C12Q1/6816 ,  G01N33/52 ,  G01N33/58

Abstract: The OH group located in the 3' and/or 2' position of a nucleoside, nucleotide or oligonucleotide is derivatised to an amino or thiol group and subsequently a fluorescent dye is coupled thereto. The resulting 3'- and/or 2'-amino- and thiol-modified nucleosides, nucleotides and oligonucleotides can then be used for the synthesis of items in the presence of a template strand or of oligonucleotides, and for the detection of genetic material. They offer the advantage that the fluorescent label is no longer attached to the 5' end of the oligonucleotide or to the nucleobase and thus does not have to be introduced during the chemical synthesis as in labelling techniques hitherto disclosed, while the known and conventional methods have the disadvantage that only some polymerases can be employed for the synthesis, the acceptance of the triphosphates by the polymerases falls and, in addition, a large substrate excess is necessary.

公开(公告)号：GR3021041T3

公开(公告)日：1996-12-31

申请号：GR960402400

申请日：1996-09-13

Applicant: HOECHST AG

Inventor： ENGELS JOACHIM ,  HERRLEIN MATHIAS ,  KONRAD RENATE ,  MAG MATTHIAS

IPC: C07H21/02 ,  A61K31/7052 ,  A61K31/7088 ,  C07H19/20 ,  C07H21/00 ,  C07H21/04 ,  C12Q1/68 ,  C12Q1/6813 ,  C12Q1/6816 ,  G01N33/52 ,  G01N33/58

Abstract: The OH group located in the 3' and/or 2' position of a nucleoside, nucleotide or oligonucleotide is derivatised to an amino or thiol group and subsequently a fluorescent dye is coupled thereto. The resulting 3'- and/or 2'-amino- and thiol-modified nucleosides, nucleotides and oligonucleotides can then be used for the synthesis of items in the presence of a template strand or of oligonucleotides, and for the detection of genetic material. They offer the advantage that the fluorescent label is no longer attached to the 5' end of the oligonucleotide or to the nucleobase and thus does not have to be introduced during the chemical synthesis as in labelling techniques hitherto disclosed, while the known and conventional methods have the disadvantage that only some polymerases can be employed for the synthesis, the acceptance of the triphosphates by the polymerases falls and, in addition, a large substrate excess is necessary.

公开(公告)号：RO111575B1

公开(公告)日：1996-11-29

申请号：RO14892691

申请日：1991-12-09

Applicant: HOECHST AG

Inventor： ENGELS JOACHIM ,  HERRLEIN MATHIAS ,  KONRAD RENATE ,  MAG MATTHIAS

IPC: C07H21/02 ,  A61K31/7052 ,  A61K31/7088 ,  C07H19/20 ,  C07H21/00 ,  C07H21/04 ,  C12Q1/68 ,  C12Q1/6813 ,  C12Q1/6816 ,  G01N33/52 ,  G01N33/58 ,  C07H19/06 ,  C07H19/16

Abstract: The OH group located in the 3' and/or 2' position of a nucleoside, nucleotide or oligonucleotide is derivatised to an amino or thiol group and subsequently a fluorescent dye is coupled thereto. The resulting 3'- and/or 2'-amino- and thiol-modified nucleosides, nucleotides and oligonucleotides can then be used for the synthesis of items in the presence of a template strand or of oligonucleotides, and for the detection of genetic material. They offer the advantage that the fluorescent label is no longer attached to the 5' end of the oligonucleotide or to the nucleobase and thus does not have to be introduced during the chemical synthesis as in labelling techniques hitherto disclosed, while the known and conventional methods have the disadvantage that only some polymerases can be employed for the synthesis, the acceptance of the triphosphates by the polymerases falls and, in addition, a large substrate excess is necessary.

公开(公告)号：CA2135591A1

公开(公告)日：1995-05-13

申请号：CA2135591

申请日：1994-11-10

Applicant: HOECHST AG

Inventor： PEYMAN ANUSCHIRWAN ,  UHLMANN EUGEN ,  MAG MATTHIAS ,  KRETZSCHMAR GERHARD ,  HELSBERG MATTHIAS ,  WINKLER IRVIN

IPC: C12N15/09 ,  A61K31/70 ,  A61K31/7088 ,  A61P31/12 ,  A61P31/22 ,  A61P35/00 ,  C07H21/00 ,  C07H21/04 ,  C12Q1/70 ,  C12Q1/68

Abstract: of the disclosure: Stabilized oligonucleotides and their use The invention relates to novel stabilized oligonucleotides in which at least one non-terminal pyrimidine nucleoside is modified, and to their use as a diagnostic or pharmaceutical for the treatment of viral infections, cancer or diseases in which integrins or cell-cell adhesion receptors are active.

公开(公告)号：CA2132265A1

公开(公告)日：1995-03-18

申请号：CA2132265

申请日：1994-09-16

Applicant: HOECHST AG

Inventor： PEYMAN ANUSCHIRWAN ,  UHLMANN EUGEN ,  MAG MATTHIAS ,  KRETZSCHMAR GERHARD ,  HELSBERG MATTHIAS ,  WINKLER IRVIN

IPC: C12N15/09 ,  A61K31/70 ,  A61P31/12 ,  A61P31/22 ,  C07H21/00 ,  C07H21/04 ,  C12N15/113 ,  A61K48/00 ,  A61K47/48

Abstract: of the disclosure Novel antisense oligonucleotides against HSV 1, and their preparation The invention relates to novel antisense oligonucleotides having the sequences AO1 (Herp099): 5'-G C G G G G C T C C A T G G G G G T C G-3' AO2 (Herp018): 5'-G C A G G A G G A T G C T G A G G A G G-3' AO3 (Herp002): 5'-G G G G C G G G G C T C C A T G G G G G-3' AO4 (Herp112): 5'-G G C G G G G C T C C A T G G G G G T C-3' AO5 (Herp034): 5'-G G G G C T C C A T G G G G G T C G T A-3' AO6 (Herp024): 5'-A A G A G G T C C A T T G G G T G G G G-3' or AO7 (Herp028): 5'-G G C C C T G C T G T T C C G T G G C G-3' and their mixtures, homologs or modified forms, against HSV 1.

公开(公告)号：HUT60505A

公开(公告)日：1992-09-28

申请号：HU388591

申请日：1991-12-10

Applicant: HOECHST AG

Inventor： ENGELS JOACHIM ,  HERRLEIN MATHIAS ,  KONRAD RENATE ,  MAG MATTHIAS

IPC: C07H21/02 ,  A61K31/7052 ,  A61K31/7088 ,  C07H19/20 ,  C07H21/00 ,  C07H21/04 ,  C12Q1/68 ,  C12Q1/6813 ,  C12Q1/6816 ,  G01N33/52 ,  G01N33/58 ,  C07H19/10 ,  C12P19/34

Abstract: The OH group located in the 3' and/or 2' position of a nucleoside, nucleotide or oligonucleotide is derivatised to an amino or thiol group and subsequently a fluorescent dye is coupled thereto. The resulting 3'- and/or 2'-amino- and thiol-modified nucleosides, nucleotides and oligonucleotides can then be used for the synthesis of items in the presence of a template strand or of oligonucleotides, and for the detection of genetic material. They offer the advantage that the fluorescent label is no longer attached to the 5' end of the oligonucleotide or to the nucleobase and thus does not have to be introduced during the chemical synthesis as in labelling techniques hitherto disclosed, while the known and conventional methods have the disadvantage that only some polymerases can be employed for the synthesis, the acceptance of the triphosphates by the polymerases falls and, in addition, a large substrate excess is necessary.

公开(公告)号：CS9103743A3

公开(公告)日：1992-06-17

申请号：CS374391

申请日：1991-12-10

Applicant: HOECHST AG

Inventor： ENGELS JOACHIM ,  HERRLEIN MATHIAS ,  KONRAD RENATE ,  MAG MATTHIAS

IPC: C07H21/02 ,  A61K31/7052 ,  A61K31/7088 ,  C07H19/20 ,  C07H21/00 ,  C07H21/04 ,  C12Q1/68 ,  G01N33/52 ,  G01N33/58 ,  C07H19/00

CPC classification number: C07H21/00 ,  C12Q1/6813 ,  C12Q1/6816 ,  Y02P20/55 ,  C12Q2525/113