公开(公告)号：US3772264A

公开(公告)日：1973-11-13

申请号：US3772264D

申请日：1971-09-17

Applicant: HOFFMANN LA ROCHE

Inventor： BAYER E ,  MUTTER M ,  GILLESSEN D ,  KUENZI H ,  STUDER R

IPC: C07C233/09 ,  C07K1/04 ,  C07K5/103 ,  C07C103/52 ,  C07G7/00 ,  C08H1/00

CPC classification number: C07K1/042 ,  C07K5/1005

Abstract: PEPTIDES ARE PREPARED IN A HOMOGENEOUS SYSTEM BY COUPLING A FIRST AMINO ACID TO A SOLUBLE POLYMER SUPPORT AND THEREAFTER COUPLING ADDITIONAL AMINO ACIDS IN DESIRED SEQUENCE UNTIL THE ULTIMATE PEPTIDE IS OBTAINED. REAGENTS USED IN THE INTERMEDIATE STEPS ARE READILY SEPARATED FROM THE COUPLED POLYMER-AMINO ACID CHAIN BY SELECTIVE FILTRATION TECHNIQUES. IN THE FINAL STEP THE DESIRED PEPTIDE IS CLEAVED FROM THE POLYMER SUPPORT AND SEPARATED FROM THE SOLUBLE POLYMER BY USE OF ULTRAFILTRATION.