公开(公告)号：ES2532734T3

公开(公告)日：2015-03-31

申请号：ES10747418

申请日：2010-08-19

Applicant: HOFFMANN LA ROCHE

Inventor： JOSEL HANS-PETER ,  GERG MICHAEL ,  HERRMANN RUPERT ,  SAEZ DIAZ ROSA ISABEL ,  NASER WERNER

IPC: G01N1/30

Abstract: Un procedimiento para la fijación de una muestra de célula o tejido in vitro en el que dicha muestra de célula o tejido se incuba con un anhídrido bismaleico según la fórmula I**Fórmula** en la que R1 y R2 se seleccionan independientemente del grupo consistente en hidrógeno, metilo, etilo, propilo, isopropilo y butilo, en la que X es un ligador de entre 1 y 30 átomos de longitud con el que se fija dicha muestra de célula o tejido.

公开(公告)号：HK1165553A1

公开(公告)日：2012-10-05

申请号：HK12106300

申请日：2012-06-28

Applicant: HOFFMANN LA ROCHE

Inventor： JOSEL HANS-PETER ,  GERG MICHAEL ,  HERRMANN RUPERT ,  SAEZ DIAZ ROSA ISABEL

IPC: G01N20060101

公开(公告)号：CA2770545A1

公开(公告)日：2011-02-24

申请号：CA2770545

申请日：2010-08-19

Applicant: HOFFMANN LA ROCHE

Inventor： JOSEL HANS-PETER ,  GERG MICHAEL ,  HERRMANN RUPERT ,  SAEZ DIAZ ROSA ISABEL

IPC: G01N1/30

Abstract: The present invention relates to novel bis-maleic anhydrides. It especially relates to the discovery that bis-maleic anhydride cross-linking agents can be used for preservation/fixation of a cell or tissue sample. With great advantage a bis-maleic anhydride cross-linking agent can be used in methods requiring fixation of a cell or tissue sample and at the same time requiring that the fixative has little impact on the later detection of a protein or a nucleic acid in procedures like immunohistochemistry, fluorescence in situ hybridization or RT-PCR.

公开(公告)号：CA2770545C

公开(公告)日：2015-11-03

申请号：CA2770545

申请日：2010-08-19

Applicant: HOFFMANN LA ROCHE

Inventor： JOSEL HANS-PETER ,  GERG MICHAEL ,  HERRMANN RUPERT ,  SAEZ DIAZ ROSA ISABEL

IPC: G01N1/30

Abstract: The present invention relates to novel bis-maleic anhydrides. It especially relates to the discovery that bis-maleic anhydride cross-linking agents can be used for preservation/fixation of a cell or tissue sample. With great advantage a bis-maleic anhydride cross-linking agent can be used in methods requiring fixation of a cell or tissue sample and at the same time requiring that the fixative has little impact on the later detection of a protein or a nucleic acid in procedures like immunohistochemistry, fluorescence in situ hybridization or RT-PCR.