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    Fluorometry for analyte by intramolecular quencher-fluorophore conjugate
    2.
    发明专利
    Fluorometry for analyte by intramolecular quencher-fluorophore conjugate 有权
    荧光分光光度计分析仪荧光分光光度计

    公开(公告)号：JP2004258021A

    公开(公告)日：2004-09-16

    申请号：JP2004017685

    申请日：2004-01-26

    Applicant: F Hoffmann La Roche Ag ,  エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft

    Inventor： HORN CARINA ,  JOSEL HANS-PETER ,  SPINKE JUERGEN ,  HERRMANN RUPERT ,  HEINDL DIETER

    IPC: G01N31/22 ,  C12Q1/26 ,  G01N21/64 ,  G01N21/78 ,  G01N33/58 ,  G01N33/66

    CPC classification number: G01N21/6428 ,  G01N2021/6432 ,  Y10S436/80 ,  Y10S436/904

    Abstract: PROBLEM TO BE SOLVED: To provide a method and a reagent kit n for fluorometry of an analyte, and a novel oxidation-reduction active compound serving as a fluorescent reagent for the analyte. SOLUTION: In this method for detecting the analyte by an oxidation-reduction reaction and the fluorometry, a sample containing the analyte is brought into contact with a detecting reagent containing a compound expressed by the following general formula (1): Q-F(Q is a quencher group, and F is a fluorophore group) as a fluorescent oxidation-reduction indicator. Examples favorable as the reducible quencher group are quinones, aromatic nitroso compounds such as nitrosoanilines and other nitrosobenzene derivatives, and N-oxides. COPYRIGHT: (C)2004,JPO&NCIPI

    Abstract translation: 要解决的问题：提供用于分析物的荧光测定的方法和试剂盒n，以及用作分析物的荧光试剂的新型氧化还原活性化合物。 解决方案：在通过氧化还原反应和荧光测定法检测分析物的方法中，使含有分析物的样品与含有由以下通式（1）表示的化合物的检测试剂接触：QF（ Q是猝灭基团，F是荧光团）作为荧光氧化还原指示剂。 可还原的猝灭剂组是醌类，亚硝基苯胺和其它亚硝基苯衍生物等芳香族亚硝基化合物，N-氧化物等。 版权所有（C）2004，JPO＆NCIPI

    IMPROVED MEASUREMENT OF VITAMIN D
    4.
    发明申请
    IMPROVED MEASUREMENT OF VITAMIN D 审中-公开
    改善维生素D的测定

    公开(公告)号：WO2007140962A3

    公开(公告)日：2008-01-31

    申请号：PCT/EP2007004924

    申请日：2007-06-04

    Applicant: ROCHE DIAGNOSTICS GMBH ,  HOFFMANN LA ROCHE ,  KOBOLD UWE ,  DUELFFER THOMAS ,  GROL MICHAEL ,  HERRMANN RUPERT ,  ELTZ HERBERT VON DER ,  VON PROFF LEOPOLD

    Inventor： KOBOLD UWE ,  DUELFFER THOMAS ,  GROL MICHAEL ,  HERRMANN RUPERT ,  VON DER ELTZ HERBERT ,  VON PROFF LEOPOLD

    IPC: G01N33/82

    CPC classification number: G01N33/82 ,  Y10T436/20 ,  Y10T436/203332

    Abstract: The present invention relates to a method of measuring a vitamin D metabolite in a sample, the method comprising the steps of (a) treating said sample with a vitamin D metabolite releasing reagent under conditions appropriate to release a vitamin D metabolite from vitamin D-binding protein and not to cause protein precipitation, (b) subjecting the treated sample obtained in step (a) to a chromatographic separation, and (c) measuring a vitamin D metabolite during or after said chromatographic separation. The present invention also relates to methods for determining the vitamin D status of a subject, for use in the diagnosis of disease, and to agents and kits for use in performing the methods of the invention.

    Abstract translation: 本发明涉及一种测量样品中维生素D代谢物的方法，所述方法包括以下步骤：（a）在适于从维生素D结合物释放维生素D代谢物的条件下用维生素D代谢物释放试剂处理所述样品 蛋白质，不会引起蛋白质沉淀，（b）使步骤（a）中得到的处理样品进行色谱分离，和（c）在所述色谱分离期间或之后测量维生素D代谢物。 本发明还涉及确定用于疾病诊断的受试者的维生素D状态的方法以及用于实施本发明的方法的试剂和试剂盒。

    DIFFERENTIAL HEMOLYSIS OF A WHOLE BLOOD SAMPLE
    6.
    发明申请
    DIFFERENTIAL HEMOLYSIS OF A WHOLE BLOOD SAMPLE 审中-公开
    全血样品的差异分析

    公开(公告)号：WO2007140961A8

    公开(公告)日：2008-11-06

    申请号：PCT/EP2007004923

    申请日：2007-06-04

    Applicant: ROCHE DIAGNOSTICS GMBH ,  HOFFMANN LA ROCHE ,  KOBOLD UWE ,  DUELFFER THOMAS ,  HERRMANN RUPERT ,  ELTZ HERBERT VON DER

    Inventor： KOBOLD UWE ,  DUELFFER THOMAS ,  HERRMANN RUPERT ,  VON DER ELTZ HERBERT

    IPC: G01N30/06 ,  G01N1/38 ,  G01N30/02 ,  G01N30/60 ,  G01N30/72 ,  G01N33/50

    CPC classification number: G01N30/06 ,  G01N30/603 ,  G01N30/7233 ,  G01N33/5002 ,  G01N2030/027 ,  Y10T436/24 ,  Y10T436/25

    Abstract: The invention relates to a method for differentially hemolyzing whole blood. It discloses method for detecting an analyte in a liquid sample known or suspected to comprise red blood cells and suspected or known to comprise eukaryotic cells, the method comprising the steps of processing said liquid sample with a membrane solubilizing agent under conditions appropriate to lyse cell membranes of red blood cells and at the same time not to cause precipitation of sample constituents, subjecting the processed sample to a chromatographic separation, and detecting the analyte. The differential hemolysis of red blood cells is of advantage in a method of detecting an analyte in a liquid sample that may comprise both erythrocytes as well as nucleated cells. The differential solubilization of red blood cells can be easily combined with an online detection methodology, like LC-MS, and is advantageous in the detection of many analytes, e.g. in the detection of folate or of immunosuppressive drugs, like tacrolimus or sirolimus.

    Abstract translation: 本发明涉及一种使全血不同溶血的方法。 它公开了用于检测已知或怀疑包含红细胞并且怀疑或已知包含真核细胞的液体样品中的分析物的方法，所述方法包括以下步骤：在适合于裂解细胞膜的条件下用膜增溶剂处理所述液体样品 的红细胞，同时不会引起样品成分沉淀，对经处理的样品进行色谱分离，并检测分析物。 在检测可能包含红细胞和有核细胞的液体样品中的分析物的方法中，红细胞的差异溶血是有利的。 红细胞的差异溶解可以容易地与诸如LC-MS的在线检测方法组合，并且在许多分析物的检测中是有利的，例如， 在检测叶酸或免疫抑制药物，如他克莫司或西罗莫司。

    REACTIVO PARA LA DIGESTION DE LA HEMOGLOBINA.
    10.
    发明专利
    REACTIVO PARA LA DIGESTION DE LA HEMOGLOBINA. 未知

    公开(公告)号：ES2347920T3

    公开(公告)日：2010-11-25

    申请号：ES07801434

    申请日：2007-07-19

    Applicant: HOFFMANN LA ROCHE

    Inventor： DUELFFER THOMAS ,  VON DER ELTZ HERBERT ,  KOBOLD UWE ,  VOGT BERND ,  HERRMANN RUPERT

    IPC: G01N33/72 ,  C12Q1/37

    Abstract: Método para la medición de HbA1c, comprendiendo el método las etapas siguientes: a) mezclar una muestra que contiene hemoglobina con un reactivo para la digestión de la hemoglobina, comprendiendo el reactivo: aa) un tampón, ab) pepsina, y ac) una sal 1,3-dialquil-imidazolio de un catión 1,3dialquil-imidazolio y un contraión. b) digerir la hemoglobina durante 1 a 60 minutos, obteniendo de esta manera un fragmento de hemoglobina que comprende los 14 aminoácidos N-terminales de HbA1c, y c) medir dicho fragmento N-terminal de HbA1c.

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