公开(公告)号：DE69728158T2

公开(公告)日：2004-10-28

申请号：DE69728158

申请日：1997-07-22

Applicant: IDEXX LAB INC

Inventor： KUMAR AMIT ,  JANG SHELDON ,  LEUNG KAI-YU ,  ROCCO MICHELE ,  PLATSHON CHARLES

IPC: G01N21/03 ,  G01N21/64 ,  G01N33/543 ,  G01N33/552 ,  G01N33/94 ,  G01N35/00 ,  B01L9/06 ,  B01L3/00 ,  G01N35/04

Abstract: A fluorescent immunoassay employing the interior surface of a capillary tube is provided. Devices to permit immunoassays using one or more capillary tubes, an apparatus for use with the devices, and a process for screening for analyte in a sample using the devices and apparatus are also provided. Samples suspected of containing analyte are added to a disposable self-contained sample tray containing one or more sample wells, mixed with a reagent, drawn into one or more spaced-apart capillary tubes held within a disposable cartridge connected to an analytical apparatus, reacted with a binding member on the surface of the capillary tube, washed to stop the reaction, and dried by the apparatus. The capillary tube is then exposed to a signal generation device to create a fluorescence signal that is detected using a signal detector. The apparatus determines the presence of the analyte and optionally determines the amount of analyte present in the sample, and presents the results to the operator.

公开(公告)号：DE69728158D1

公开(公告)日：2004-04-22

申请号：DE69728158

申请日：1997-07-22

Applicant: IDEXX LAB INC

Inventor： KUMAR AMIT ,  JANG SHELDON ,  LEUNG KAI-YU ,  ROCCO MICHELE ,  PLATSHON CHARLES

IPC: G01N21/03 ,  G01N21/64 ,  G01N33/543 ,  G01N33/552 ,  G01N33/94 ,  G01N35/00 ,  B01L9/06 ,  B01L3/00 ,  G01N35/04

Abstract: A fluorescent immunoassay employing the interior surface of a capillary tube is provided. Devices to permit immunoassays using one or more capillary tubes, an apparatus for use with the devices, and a process for screening for analyte in a sample using the devices and apparatus are also provided. Samples suspected of containing analyte are added to a disposable self-contained sample tray containing one or more sample wells, mixed with a reagent, drawn into one or more spaced-apart capillary tubes held within a disposable cartridge connected to an analytical apparatus, reacted with a binding member on the surface of the capillary tube, washed to stop the reaction, and dried by the apparatus. The capillary tube is then exposed to a signal generation device to create a fluorescence signal that is detected using a signal detector. The apparatus determines the presence of the analyte and optionally determines the amount of analyte present in the sample, and presents the results to the operator.