公开(公告)号：WO1998030873A1

公开(公告)日：1998-07-16

申请号：PCT/US1997023995

申请日：1997-12-30

Applicant: INPHOCYTE, INC.

Inventor： INPHOCYTE, INC. ,  ZAKIM, David, S. ,  CHIROBOGA, Luis ,  DIEM, Max ,  XIE, Ping

IPC: G01J03/28

CPC classification number: G01J3/453 ,  G01J3/44

Abstract: A system and method is described for permitting the simultaneaous automated collection of data with regard to the presence of diseased cells, in particular neoplastic, dysplastic or cancerous cells, or normal cells at a given site in a section of tissue. This is accomplished according to the following steps. First, IR spectral data are collected simultaneously for each cell or pixel of the tissue. The present invention utilizes step scanning Fourier transform technology, where a moveable interferometer is used as a spectral filter. Next, these data are analyzed by identifying the cell types encountered in the sample by comparing them with reference data sets for healthy and diseased cells. Finally, a false color mapping of the distribution of different types of cells in the tissue section is presented that permits assessment of the prevalence of diseased cells.

Abstract translation: 描述了一种系统和方法，用于允许在组织的一部分中给定部位处存在患病细胞，特别是肿瘤，发育异常或癌细胞或正常细胞的同时自动收集数据。 这是按照以下步骤完成的。 首先，对于组织的每个单元或像素同时收集IR光谱数据。 本发明利用了步进扫描傅里叶变换技术，其中使用可移动干涉仪作为光谱滤波器。 接下来，通过将样本中遇到的细胞类型与健康和患病细胞的参考数据集进行比较来分析这些数据。 最后，提出了组织切片中不同类型细胞分布的虚拟色图，可以评估患病细胞的流行程度。