公开(公告)号：WO1997030338A1

公开(公告)日：1997-08-21

申请号：PCT/US1997001625

申请日：1997-02-04

Applicant: INPHOCYTE, INC.

Inventor： INPHOCYTE, INC. ,  ZAKIM, David, S. ,  DIEM, Max

IPC: G01N15/14

CPC classification number: G01N21/65 ,  G01N15/1459 ,  G01N2015/1486 ,  G01N2015/149 ,  G01N2021/651

Abstract: A system and method of detecting diseased cells in a large population of normal cells that uses flow cytometry and vibrational spectroscopy techniques. The system and method combine flow cytometry with a spectrometric system to detect inherent properties of each cell examined, inherent properties that not only discriminate between the presence or absence of disease but also the degree at which the cell is diseased. More specifically, the system and method combine the use of flow cytometry to separate cells of the sample population and Raman or resonance Raman spectroscopic techniques for detecting inherent differences in cells for the purpose of determining whether cells are diseased and level of disease in the cells. This is accomplished by forming a stream of cells by flow cytometry into the sampling region at which each cell is irradiated by an incident beam of a Raman laser. The scattered Raman spectrum from each cell is detected by a Raman spectroscopy. More specifically, a complete Raman spectrum or resonance Raman spectrum is detected for each cell flowing past the incident beam. This will provide information as to whether the specific cell in the beam of the incident light is normal or diseased, and the level of disease also may be determined on the details of the spectrum detected.

Abstract translation: 一种使用流式细胞仪和振动光谱技术检测大量正常细胞中病变细胞的系统和方法。 该系统和方法将流式细胞术与光谱系统结合，以检测所检测的每个细胞的固有性质，其不仅区分疾病的存在或不存在，而且区分疾病的程度的固有性质。 更具体地，该系统和方法将流式细胞术的使用结合到分离样品群体的细胞和用于检测细胞内固有差异的拉曼或共振拉曼光谱技术，以确定细胞是否患病和细胞中的疾病水平。 这是通过流式细胞术将细胞流形成到每个细胞被拉曼激光的入射束照射的采样区域来实现的。 通过拉曼光谱法检测每个电池的散射拉曼光谱。 更具体地，对于流过入射光束的每个单元，检测完整的拉曼光谱或共振拉曼光谱。 这将提供关于入射光束中的特定细胞是正常还是患病的信息，并且还可以根据所检测的光谱的细节来确定疾病的水平。

公开(公告)号：WO1998030873A1

公开(公告)日：1998-07-16

申请号：PCT/US1997023995

申请日：1997-12-30

Applicant: INPHOCYTE, INC.

Inventor： INPHOCYTE, INC. ,  ZAKIM, David, S. ,  CHIROBOGA, Luis ,  DIEM, Max ,  XIE, Ping

IPC: G01J03/28

CPC classification number: G01J3/453 ,  G01J3/44

Abstract: A system and method is described for permitting the simultaneaous automated collection of data with regard to the presence of diseased cells, in particular neoplastic, dysplastic or cancerous cells, or normal cells at a given site in a section of tissue. This is accomplished according to the following steps. First, IR spectral data are collected simultaneously for each cell or pixel of the tissue. The present invention utilizes step scanning Fourier transform technology, where a moveable interferometer is used as a spectral filter. Next, these data are analyzed by identifying the cell types encountered in the sample by comparing them with reference data sets for healthy and diseased cells. Finally, a false color mapping of the distribution of different types of cells in the tissue section is presented that permits assessment of the prevalence of diseased cells.

Abstract translation: 描述了一种系统和方法，用于允许在组织的一部分中给定部位处存在患病细胞，特别是肿瘤，发育异常或癌细胞或正常细胞的同时自动收集数据。 这是按照以下步骤完成的。 首先，对于组织的每个单元或像素同时收集IR光谱数据。 本发明利用了步进扫描傅里叶变换技术，其中使用可移动干涉仪作为光谱滤波器。 接下来，通过将样本中遇到的细胞类型与健康和患病细胞的参考数据集进行比较来分析这些数据。 最后，提出了组织切片中不同类型细胞分布的虚拟色图，可以评估患病细胞的流行程度。

公开(公告)号：WO1997030340A1

公开(公告)日：1997-08-21

申请号：PCT/US1997001890

申请日：1997-02-04

Applicant: INPHOCYTE, INC.

Inventor： INPHOCYTE, INC. ,  ZAKIM, David, S. ,  DIEM, Max

IPC: G01N21/35

CPC classification number: G01N1/4077 ,  G01N1/2813 ,  G01N21/03 ,  G01N21/35

Abstract: A biological cell sample holder (100) for use in vibrational spectroscopy. The sample holder includes a rectangular body (102) that has a central relief area (104) and an opening (106) through the relief area. A porous membrane (108) is disposed in the opening and a metal coated disk (110) is disposed on top of the porous membrane. The disk has pores of a predetermined size to allow fluid to pass through the disk but retain cells of interest on the disk.

Abstract translation: 用于振动光谱的生物细胞样品架（100）。 样品架包括具有中心浮雕区域（104）和穿过浮雕区域的开口（106）的矩形体（102）。 多孔膜（108）设置在开口中，金属涂覆的盘（110）设置在多孔膜的顶部。 盘具有预定尺寸的孔，以允许流体通过盘，但将感兴趣的细胞保留在盘上。