公开(公告)号：WO2015163733A1

公开(公告)日：2015-10-29

申请号：PCT/KR2015/004132

申请日：2015-04-24

Applicant: INSTITUTE FOR BASIC SCIENCE

Inventor： KIM, Jin Soo ,  BAE, Sang Su

IPC: C12Q1/68 ,  G06F19/22

CPC classification number: G06F19/24 ,  G06F19/18

Abstract: The present invention relates to a method of selecting a nuclease target sequence for gene knockout based on microhomology.

Abstract translation: 本发明涉及一种基于微生物学选择基因敲除的核酸酶靶序列的方法。

公开(公告)号：EP3219810A1

公开(公告)日：2017-09-20

申请号：EP15858194.2

申请日：2015-11-13

Applicant: Institute for Basic Science

Inventor： KIM, Jin Soo ,  KIM, Dae Sik ,  BAE, Sang Su

IPC: C12Q1/68 ,  C12N9/22 ,  G06F19/22

CPC classification number: C12N9/22 ,  C12Q1/68 ,  G06F19/22 ,  Y02A50/451

Abstract: The present disclosure relates to a method for detecting off-target sites of a programmable nuclease in a genome, and specifically, to a method for detecting off-target sites through data analysis by subjecting the genome isolated in vitro to programmable nucleases to cleave the genome and then performing whole genome sequencing or deep sequencing, and to a method for selecting on-target sites of a programmable nuclease, which minimizes the off-target effect, using this method. The Digenome-seq of the present disclosure can detect the off-target sites of a programmable nuclease on the genomic scale at a high degree of reproducibility, and thus can be used in the manufacture of programmable nucleases having high target specificity and the study thereof.

Abstract translation: 本发明涉及用于检测基因组中可编程核酸酶的脱靶位点的方法，具体涉及通过数据分析检测脱靶位点的方法，所述方法通过使体外分离的基因组经受可程序化的核酸酶以切割基因组 然后进行全基因组测序或深度测序，以及使用该方法选择使可脱靶效应最小化的可编程核酸酶的在靶位点的方法。 本公开的Digenome-seq可以高度重现性地在基因组规模上检测可编程核酸酶的脱靶位点，并因此可用于制备具有高靶特异性的可编程核酸酶及其研究。