公开(公告)号：EP0463755A1

公开(公告)日：1992-01-02

申请号：EP91305127.2

申请日：1991-06-06

Applicant: INSTRUMENTATION LABORATORY S.p.A.

Inventor： San George, Richard C. ,  Adiletto, Carol A.

IPC: C12Q1/00 ,  C12Q1/32 ,  C12Q1/52

CPC classification number: C12Q1/32 ,  C12Q1/008 ,  C12Q1/52 ,  Y10S435/81 ,  Y10S435/975 ,  Y10T436/107497

Abstract: An aqueous coenzyme reagent composition contains NADH, a carbonate/bicarbonate buffer (with a pH of about 9.5 - 11) and water. Both NADH and the buffer are at low concentrations, e.g., about 2 - 5 mM for NADH and about 2 - 15 mM for carbonate/bicarbonate. When this coenzyme reagent is mixed with an enzyme reagent, the mixture achieves a neutral pH. The combination of high pH, low NADH concentration and low buffer concentration permit the aqueous reagent to be stored for extended periods at low temperatures without NADH degrading to form impurities which interfere with or inhibit enzyme activity, such as the activity of lactate dehydrogenase or malate dehydrogenase in an assay for ALT or AST.

Abstract translation: 含水辅酶试剂组合物含有NADH，碳酸盐/碳酸氢盐缓冲液（pH约9.5-11）和水。 NADH和缓冲液均为低浓度，例如对于NADH为约2-5mM，对于碳酸盐/碳酸氢盐为约2-15mM。 当该辅酶试剂与酶试剂混合时，混合物达到中性pH。 高pH值，低NADH浓度和低缓冲浓度的组合允许水性试剂在低温下长时间储存​​，而不会使NADH降解以形成干扰或抑制酶活性的杂质，例如乳酸脱氢酶或苹果酸脱氢酶的活性 在ALT或AST的检测中。