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    THROMBOSIS RISK TEST
    6.
    发明申请
    THROMBOSIS RISK TEST 审中-公开
    血栓风险测试

    公开(公告)号：WO1996042018A1

    公开(公告)日：1996-12-27

    申请号：PCT/US1996009036

    申请日：1996-06-06

    Applicant: INSTRUMENTATION LABORATORY, S.P.A. ,  CAMPBELL, Paula, A. ,  PREDA, Luigi

    Inventor： INSTRUMENTATION LABORATORY, S.P.A.

    IPC: G01N33/86

    CPC classification number: G01N33/86 ,  G01N2333/4626 ,  G01N2333/745 ,  G01N2333/96461 ,  G01N2333/96463 ,  G01N2405/04

    Abstract: The present invention provides a method for determining thrombotic risk in an individual. The method involves determining the activity of Protein C and Protein S in the plasma of the individual thought to be at thrombotic risk by adding to a plasma sample obtained from the individual: (i) a first reagent in an amount sufficient to induce or activate coagulation in the plasma, (ii) a second reagent which activates endogenous protein C in the plasma, and (iii) a third reagent comprising calcium salts, phospholipids or tissue thromboplastin, or a combination thereof. To a second plasma sample from the same subject is added a reagent which induces or activates coagulation, and a buffer or other material which does not activate protein C, and a third reagent as described above. The time, rate or both, necessary for the conversion of endogenous fibrinogen to fibrin in both the first and second samples is measured. The same steps are performed on normal control plasma, and the difference or ratio in the times, rates, or both, obtained above are determined. The difference or ratio is indicative of the thrombotic risk in the subject. A kit adapted to carry out the method also is the subject of the present invention. The methods and kits of the invention in other embodiments may comprise a first reagent comprising a synthetic substrate, a second reagent which in the first sample from the subject activates protein C, and in the second sample, a second reagent which does not activate protein C. In these embodiments, the rates of hydrolysis of the synthetic substrates are measured and compared.

    Abstract translation: 本发明提供了确定个体血栓形成风险的方法。 该方法包括通过加入从个体得到的血浆样品来确定被认为是血栓形成风险的个体的血浆中的蛋白C和蛋白S的活性：（i）足以诱导或激活凝血的量的第一试剂 在等离子体中，（ii）活化血浆中的内源蛋白C的第二试剂，和（iii）包含钙盐，磷脂或组织凝血激酶或其组合的第三试剂。 向来自相同受试者的第二血浆样品中加入诱导或活化凝血剂的试剂，以及不活化蛋白质C的缓冲液或其它物质，以及如上所述的第三试剂。 测量在第一和第二样品中将内源纤维蛋白原转化为纤维蛋白所需的时间，速率或两者。 对正常对照等离子体进行相同的步骤，并确定上述得到的时间，速率或二者的差异或比例。 差异或比例表明受试者的血栓形成风险。 适于实施该方法的套件也是本发明的主题。 在其它实施方案中，本发明的方法和试剂盒可以包括第一试剂，其包含合成底物，来自受试者的第一样品中的第二试剂激活蛋白C，而在第二样品中，第二试剂不会活化蛋白C 在这些实施方案中，测量和比较合成基材的水解速率。

    Method for producing a liquid phase calibration substance having a pre-established concentration of CO2 suitable for calibrating analytical instruments such as hemogasanalyzers
    9.
    发明公开
    Method for producing a liquid phase calibration substance having a pre-established concentration of CO2 suitable for calibrating analytical instruments such as hemogasanalyzers 失效
    一种用于在液相中制备Eichungssubstanz，与CO 2预先固定浓度，其适合于分析仪器如血液气体分析装置的校准过程。

    公开(公告)号：EP0530871A1

    公开(公告)日：1993-03-10

    申请号：EP92202343.7

    申请日：1992-07-29

    Applicant: INSTRUMENTATION LABORATORY S.p.A.

    Inventor： Tancredi, Gabrio ,  Calzi, Claudio

    IPC: G01N33/00 ,  G01N27/416 ,  G01N33/49

    CPC classification number: G01N33/4925 ,  G01N27/4163 ,  G01N33/0006 ,  Y10T436/10 ,  Y10T436/100833 ,  Y10T436/102499 ,  Y10T436/106664 ,  Y10T436/108331 ,  Y10T436/109163

    Abstract: A method for producing a liquid phase substance having a pre-established concentration of CO₂ suitable for calibrating analytical instruments such as hemogasanalyzers consists in:

    preparing an aqueous solution of precisely known strength of a water-soluble salt of carbonic acid (bicarbonate or alkaline carbonate),
    then transferring said solution to a flow reactor containing a cation exchange resin in hydrogenionic form,
    passing the aforesaid solution of carbonic acid salt through the aforesaid cation resin,
    thereby achieving the reaction of producing a pre-established quantity of CO₂, and
    transferring the eluate thus obtained directly to the analytical instrument for calibration.

    Abstract translation: 如hemogasanalyzers besteht中：一种制造具有适合用于校准分析仪器CO 2的预先建立的浓度的液体相物质的方法准备wässrige的碳酸的水溶性盐的精确的已知浓度的溶液（碳酸氢盐或碱金属碳酸盐） ，然后转移环所述溶液的流动反应器包含在hydrogenionic形式的阳离子交换树脂，使碳酸盐的上述溶液通过上述阳离子树脂，由此实现生产CO 2的预先建立的量的反应，并传递环的洗脱液 从而直接获得用于校准的分析仪器。 我

    Stabilization of glucose oxidase enzyme in liquid reagent
    10.
    发明公开
    Stabilization of glucose oxidase enzyme in liquid reagent 失效
    稳定葡萄糖 - 氧化酶 - 酶在flüssigemReagenz。

    公开(公告)号：EP0418940A1

    公开(公告)日：1991-03-27

    申请号：EP90202129.4

    申请日：1990-08-04

    Applicant: INSTRUMENTATION LABORATORY S.p.A.

    Inventor： D'Alterio, Maurizio ,  Frontini, Dario

    IPC: C12N9/96 ,  C12N9/04

    CPC classification number: C12N9/96

    Abstract: The stabilization of glucose oxidase in liquid reagent compositions for laboratory use is attained by having a phosphate buffer in a concentration of 400 to 3500 mM per liter of the liquid reagent. Such phosphate concentration exceeds by a least 100% that normally used to buffer the pH value.

    Abstract translation: 用于实验室用途的液体试剂组合物中葡萄糖氧化酶的稳定是通过使用浓度为每升液体试剂400至3500mM的磷酸盐缓冲液来实现的。 这种磷酸盐浓度超过通常用于缓冲pH值的至少100％。

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