公开(公告)号：US20160251645A1

公开(公告)日：2016-09-01

申请号：US15056026

申请日：2016-02-29

Applicant: INVISTA North America S.a.r.l.

Inventor： ADRIANA L. BOTES ,  Nadia Kadi ,  Mihai Luchian Azoitei ,  Yih-En A. Ban ,  Daniela Grabs-Röthlisberger ,  Alexander Pisarchik ,  Alexandre Zanghellini ,  Eric Althoff

IPC: C12N9/88 ,  C07K16/40 ,  G01N33/573 ,  C12P5/00 ,  C08F36/06 ,  C07C11/18 ,  C07C11/21 ,  C08F36/08 ,  C08F36/22 ,  C12P5/02 ,  C07C11/167

Abstract: The present disclosure provides novel polypeptides with 3-buten-2-ol dehydratase activity, polypeptides with catalytic activity in the conversion of 3-methyl-3-buten-2-ol to isoprene, and crystal structure data for one of such polypeptides. Methods of making and using the polypeptides and their related crystal structure data are also provided.

公开(公告)号：US20160251644A1

公开(公告)日：2016-09-01

申请号：US15055493

申请日：2016-02-26

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： ADRIANA L. BOTES ,  Nadia Kadi

IPC: C12N9/88 ,  C11B9/00 ,  C08F136/06 ,  C07K16/40 ,  C12P5/02 ,  C08F136/08 ,  C07C11/21 ,  C07C11/18 ,  C07C11/167 ,  A61K31/01 ,  C08F136/22

CPC classification number: C12N9/88 ,  A61K31/01 ,  C07C11/167 ,  C07C11/18 ,  C07C11/21 ,  C07K16/40 ,  C08F136/06 ,  C08F136/08 ,  C08F136/22 ,  C11B9/0007 ,  C12P5/026 ,  C12Y402/01 ,  C12Y402/01127

Abstract: The present disclosure provides novel polypeptides with improved 3-buten-2-ol dehydratase activity, polypeptides with improved linalool dehydratase activity, and polypeptides with catalytic activity in the conversion of 3-methyl-3-buten-2-ol to isoprene. Methods of making and using the polypeptides are also provided.

Abstract translation: 本公开提供具有改进的3-丁烯-2-醇脱水酶活性的新型多肽，具有改进的里哪醇脱水酶活性的多肽和在3-甲基-3-丁烯-2-醇转化为异戊二烯时具有催化活性的多肽。 还提供了制备和使用多肽的方法。