公开(公告)号：WO2015195678A1

公开(公告)日：2015-12-23

申请号：PCT/US2015/036050

申请日：2015-06-16

Applicant: INVISTA Technologies S.à r.l.

Inventor： BOTES, Adriana Leonora ,  CONRADIE, Alex Van Eck ,  HADDOUCHE, Ramdane

IPC: C12P7/42

CPC classification number: C12P13/001 ,  C07C47/02 ,  C07C47/12 ,  C07C59/01 ,  C07C59/325 ,  C07C223/02 ,  C08G63/06 ,  C12N9/0008 ,  C12N9/0077 ,  C12N9/1007 ,  C12N9/1096 ,  C12P7/24 ,  C12P7/40 ,  C12P7/42 ,  C12P7/44 ,  C12P7/62 ,  C12P7/6409 ,  C12P7/6436 ,  C12Y102/99006 ,  C12Y114/15001 ,  C12Y114/15003 ,  C12Y206/01018 ,  C12Y206/01019 ,  C12Y206/01029 ,  C12Y206/01048 ,  C12Y206/01082

Abstract: This document describes biochemical pathways for producing 7-hydroxyheptanoate methyl ester and heptanoic acid heptyl ester using one or more of a fatty acid O-methyltransferase , an alcohol O-acetyltransferase , and a monooxygenase , as well as recombinant hosts expressing one or more of such exogenous enzymes. 7-hydroxyheptanoate methyl esters and heptanoic acid heptyl esters can be enzymatically converted to pimelic acid, 7-aminoheptanoate, 7-hydroxyheptanoate, heptamethylenediamine, or 1,7-heptanediol.

Abstract translation: 本文件描述了使用一种或多种脂肪酸O-甲基转移酶，醇O-乙酰转移酶和单加氧酶来生产7-羟基庚酸甲酯和庚酸庚酯的生物化学途径，以及表达一种或多种这样的 外源酶。 7-羟基庚酸甲酯和庚酸庚酯可以酶促转化成庚二酸，7-氨基庚酸酯，7-羟基庚酸酯，七亚甲基二胺或1,7-庚二醇。

公开(公告)号：WO2015021045A3

公开(公告)日：2015-02-12

申请号：PCT/US2014/049786

申请日：2014-08-05

Applicant: INVISTA TECHNOLOGIES S.À R.L.

Inventor： BOTES, Adriana Leonora ,  CONRADIE, Alex Van Eck

IPC: C12P5/00 ,  C12P5/02 ,  C12N15/70 ,  C12N9/10 ,  C12N9/12 ,  C12N15/52 ,  C12N9/88

Abstract: This document describes biochemical pathways for producing isoprene by forming two vinyl groups in a central precursor produced from isobutyryl-CoA, 3-methyl-2-oxopentanoate, or 4-methyl-2-oxopentanoate as well as recombinant hosts for producing isoprene.

公开(公告)号：EP3237626A2

公开(公告)日：2017-11-01

申请号：EP15882902.8

申请日：2015-12-21

Applicant: Invista Technologies S.à r.l.

Inventor： BOTES, Adriana Leonora ,  CONRADIE, Alex Van Eck

IPC: C12P7/26 ,  C12P7/42 ,  C12P7/62 ,  C07H19/207

CPC classification number: C12P19/32 ,  C07H1/00 ,  C07H19/207 ,  C12N9/1029 ,  C12P7/18 ,  C12P7/24 ,  C12P7/26 ,  C12P7/42 ,  C12P7/44 ,  C12P13/001 ,  C12P13/005 ,  C12P17/10 ,  C12Y203/01

Abstract: This document describes materials and methods for, for example, producing 6-hydroxyhexanoic acid using a β-ketothiolase or synthase and an alcohol O-acetyltransferase to form a 6-acetyloxy-3-oxohexanoyl-CoA intermediate. This document describes biochemical pathways for producing 6-hydroxyhexanoic acid using a β-ketothiolase or synthase and an alcohol O-acetyltransferase to form a 6-acetyloxy-3-oxohexanoyl-CoA intermediate. 6-hydroxyhexanoic acid can be enzymatically converted to adipic acid, caprolactam, 6-aminohexanoic acid, hexamethylenediamine or 1,6-hexanediol. This document also describes recombinant hosts producing 6-hydroxyhexanoic acid as well as adipic acid, caprolactam, 6-aminohexanoic acid, hexamethylenediamine and 1,6-hexanediol.

Abstract translation: 该文献描述了例如使用β-酮硫解酶或合酶和醇O-乙酰转移酶生成6-羟基己酸以形成6-乙酰氧基-3-氧代己酰基-CoA中间体的材料和方法。 该文献描述了使用β-酮硫解酶或合酶和醇O-乙酰转移酶生成6-羟基己酸以形成6-乙酰氧基-3-氧代己酰-CoA中间体的生物化学途径。 6-羟基己酸可以酶促转化为己二酸，己内酰胺，6-氨基己酸，六亚甲基二胺或1,6-己二醇。 该文件还描述了生产6-羟基己酸以及己二酸，己内酰胺，6-氨基己酸，六亚甲基二胺和1,6-己二醇的重组宿主。

公开(公告)号：EP3224365A1

公开(公告)日：2017-10-04

申请号：EP15805044.3

申请日：2015-11-20

Applicant: Invista Technologies S.à r.l.

Inventor： BOTES, Adriana Leonora ,  CONRADIE, Alex Van Eck ,  HADDOUCHE, Ramdane

IPC: C12P7/42 ,  C12P7/44 ,  C12P7/64 ,  C12N9/02

CPC classification number: C12N9/0071 ,  C07C47/12 ,  C07C47/19 ,  C07C49/185 ,  C07C223/02 ,  C07C229/08 ,  C07C229/12 ,  C12N9/0008 ,  C12N9/0073 ,  C12N9/18 ,  C12N9/20 ,  C12P7/42 ,  C12P7/44 ,  C12P7/6409 ,  C12Y114/13 ,  C12Y114/14001 ,  C12Y301/01001 ,  C12Y301/01003

Abstract: This document describes biochemical pathways for producing 6-hydroxyhexanoic acid using a
monooxygenase to form a 7-hydroxyoctanoate intermediate, which can be converted to 6-hydroxyhexanoate using a polypeptide having
monooxygenase ,
secondary alcohol dehydrogenase , or
esterase activity. 6-hydroxyhexanoic acid can be enzymatically converted to adipic acid, caprolactam, 6-aminohexanoic acid, hexamethylenediamine or 1,6-hexanediol. This document also describes recombinant hosts producing 6-hydroxyhexanoic acid as well as adipic acid, caprolactam, 6-aminohexanoic acid, hexamethylenediamine and 1,6-hexanediol.

Abstract translation: 该文献描述了使用单加氧酶生成6-羟基己酸以形成7-羟基辛酸酯中间体的生物化学途径，其可以使用具有单加氧酶，仲醇脱氢酶或酯酶活性的多肽转化成6-羟基己酸酯。 6-羟基己酸可以酶促转化为己二酸，己内酰胺，6-氨基己酸，六亚甲基二胺或1,6-己二醇。 该文件还描述了生产6-羟基己酸以及己二酸，己内酰胺，6-氨基己酸，六亚甲基二胺和1,6-己二醇的重组宿主。

公开(公告)号：EP3218505A1

公开(公告)日：2017-09-20

申请号：EP15804257.2

申请日：2015-11-13

Applicant: Invista Technologies S.à r.l.

Inventor： BOTES, Adriana Leonora ,  CONRADIE, Alex Van Eck ,  KADI, Nadia

IPC: C12P7/42 ,  C12P13/00 ,  C12P7/18 ,  C12P7/44 ,  C12P17/10 ,  C12N9/10

CPC classification number: C12P19/32 ,  C07C47/12 ,  C07C47/19 ,  C07C225/06 ,  C07C229/08 ,  C07C233/31 ,  C07F9/65616 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/88 ,  C12N15/52 ,  C12P7/18 ,  C12P7/42 ,  C12P7/44 ,  C12P13/001 ,  C12P13/005 ,  C12P17/10 ,  C12Y101/01035 ,  C12Y101/01036 ,  C12Y101/011 ,  C12Y101/01157 ,  C12Y102/99006 ,  C12Y103/01008 ,  C12Y103/01038 ,  C12Y103/01044 ,  C12Y203/01016 ,  C12Y203/01174 ,  C12Y206/01 ,  C12Y402/01017 ,  C12Y402/01119

Abstract: This document describes biochemical pathways for producing 6-hydroxyhexanoic acid using a polypeptide having
ß-ketothiolase activity to form a 3-oxo-6-hydroxyhexanoyl-CoA intermediate. 6-hydroxyhexanoic acid can be enzymatically converted to adipic acid, caprolactam, 6-aminohexanoic acid, hexamethylenediamine or 1,6-hexanediol. This document also describes recombinant hosts producing 6-hydroxyhexanoic acid as well as adipic acid, caprolactam, 6-aminohexanoic acid, hexamethylenediamine and 1,6-hexanediol.

Abstract translation: 该文献描述了使用具有β-酮硫解酶活性的多肽生成6-羟基己酸以形成3-氧代-6-羟基己酰基-CoA中间体的生物化学途径。 6-羟基己酸可以酶促转化为己二酸，己内酰胺，6-氨基己酸，六亚甲基二胺或1,6-己二醇。 该文件还描述了生产6-羟基己酸以及己二酸，己内酰胺，6-氨基己酸，六亚甲基二胺和1,6-己二醇的重组宿主。

公开(公告)号：EP3155110A1

公开(公告)日：2017-04-19

申请号：EP15731806.4

申请日：2015-06-16

Applicant: Invista Technologies S.à r.l.

Inventor： BOTES, Adriana Leonora ,  CONRADIE, Alex Van Eck ,  HADDOUCHE, Ramdane

IPC: C12P7/42

CPC classification number: C12P13/001 ,  C07C47/02 ,  C07C47/12 ,  C07C59/01 ,  C07C59/325 ,  C07C223/02 ,  C08G63/06 ,  C12N9/0008 ,  C12N9/0077 ,  C12N9/1007 ,  C12N9/1096 ,  C12P7/24 ,  C12P7/40 ,  C12P7/42 ,  C12P7/44 ,  C12P7/62 ,  C12P7/6409 ,  C12P7/6436 ,  C12Y102/99006 ,  C12Y114/15001 ,  C12Y114/15003 ,  C12Y206/01018 ,  C12Y206/01019 ,  C12Y206/01029 ,  C12Y206/01048 ,  C12Y206/01082

Abstract: This document describes biochemical pathways for producing 7-hydroxyheptanoate methyl ester and heptanoic acid heptyl ester using one or more of a fatty acid O-methyltransferase, an alcohol O-acetyltransferase, and a monooxygenase, as well as recombinant hosts expressing one or more of such exogenous enzymes. 7-hydroxyheptanoate methyl esters and heptanoic acid heptyl esters can be enzymatically converted to pimelic acid, 7-aminoheptanoate, 7-hydroxyheptanoate, heptamethylenediamine, or 1,7-heptanediol.

公开(公告)号：EP3155090A1

公开(公告)日：2017-04-19

申请号：EP15741382.4

申请日：2015-06-16

Applicant: Invista Technologies S.à r.l.

Inventor： BOTES, Adriana Leonora ,  CONRADIE, Alex Van Eck

IPC: C12N1/32 ,  C12N9/00

CPC classification number: C12N9/0008 ,  C07C229/08 ,  C12N9/0022 ,  C12N9/0069 ,  C12N9/1096 ,  C12N9/80 ,  C12N9/88 ,  C12P7/18 ,  C12P7/42 ,  C12P7/46 ,  C12P13/005 ,  C12Y102/01003 ,  C12Y102/01004 ,  C12Y104/03021 ,  C12Y113/12002 ,  C12Y206/01 ,  C12Y305/0103 ,  C12Y401/01

Abstract: This document describes biochemical pathways for producing glutaric acid, 5 aminopentanoic acid, 5-hydroxypentanoic acid or 1,5-pentanediol by forming one or two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C5 backbone substrate such as cadaverine or 5-aminopentanamide.

Abstract translation: 该文献描述了通过在C5主链底物如（例如）N-乙酰半胱氨酸中形成包含羧基，胺或羟基的一个或两个末端官能团来生产戊二酸，5-氨基戊酸，5-羟基戊酸或1,5-戊二醇的生物化学途径 尸胺或5-氨基戊酰胺。

公开(公告)号：EP3143152A1

公开(公告)日：2017-03-22

申请号：EP15727164.4

申请日：2015-05-15

Applicant: Invista Technologies S.à r.l.

Inventor： BOTES, Adriana Leonora ,  CONRADIE, Alex Van Eck

IPC: C12P13/04 ,  C12N15/52

CPC classification number: C12P13/001 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1096 ,  C12N9/88 ,  C12N15/52 ,  C12P7/42 ,  C12P13/005 ,  C12P13/04 ,  C12Y102/99006 ,  C12Y103/01031 ,  C12Y402/01 ,  C12Y403/01

Abstract: This document describes biochemical pathways for producing 2- aminopimelate from 2,6-diaminopimelate, and methods for converting 2- aminopimelate to one or more of adipic acid, adipate semialdehyde, caprolactam, 6- aminohexanoic acid, 6-hexanoic acid, hexamethylenediamine, or 1,6-hexanediol by decarboxylating 2-aminopimelate into a six carbon chain aliphatic backbone and enzymatically forming one or two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in the backbone.

Abstract translation: 该文件描述了由2,6-二氨基庚二酸制备2- aminopimelate生化途径和方法用于2 aminopimelate转换成一个或多个己二酸，己二酸半醛，己内酰胺，6-氨基己酸，6-己酸，六亚甲基二胺，或 1,6-己二醇通过脱羧-2- aminopimelate到六个碳链的脂族主链和酶形成一个或两个末端的官能团，包括在主链羧基，胺或羟基基团。

公开(公告)号：EP3030667A2

公开(公告)日：2016-06-15

申请号：EP14750966.5

申请日：2014-08-05

Applicant: Invista Technologies S.à r.l.

Inventor： BOTES, Adriana Leonora ,  CONRADIE, Alex Van Eck

IPC: C12P5/00 ,  C12P5/02 ,  C12N15/70 ,  C12N9/10 ,  C12N9/12 ,  C12N15/52 ,  C12N9/88

CPC classification number: C12P5/007 ,  C12N9/1029 ,  C12N9/1205 ,  C12N9/88 ,  C12N15/52 ,  C12N15/70 ,  C12P5/026 ,  C12Y203/01041 ,  Y02E50/343

Abstract: This document describes biochemical pathways for producing isoprene by forming two vinyl groups in a central precursor produced from isobutyryl-CoA, 3-methyl-2-oxopentanoate, or 4-methyl-2-oxopentanoate as well as recombinant hosts for producing isoprene.

公开(公告)号：EP3143149A1

公开(公告)日：2017-03-22

申请号：EP15725180.2

申请日：2015-05-13

Applicant: Invista Technologies S.à r.l.

Inventor： BOTES, Adriana Leonora ,  CONRADIE, Alex Van Eck

IPC: C12P7/40 ,  C12N15/63 ,  C12P7/44 ,  C12P7/62 ,  C12P17/10

CPC classification number: C12P13/001 ,  C08G83/00 ,  C12N15/52 ,  C12P7/04 ,  C12P7/40 ,  C12P7/42 ,  C12P7/44 ,  C12P7/625 ,  C12P17/10 ,  C12Y102/99006 ,  C12Y206/01

Abstract: This document describes biochemical pathways for producing adipyl-[acp] and either hexanoic acid or acetic acid from a long chain acyl-[acp] such as dodecanoyl-[acp] or octanoyl-[acp] using a polypeptide having pimeloyl-[acp] synthase activity and biochemical pathways for converting adipyl-[acp] and/or hexanoic acid to one of more of adipic acid, 6-aminohexanoic acid, 6-hydroxyhexanoic acid, hexamethylenediamine, caprolactam, and 1,6-hexanediol.

Abstract translation: 该文献描述的生物化学途径的制造己二酰[ACP]和任己酸或乙酸从一个长链酰基[ACP]：如十二烷[ACP]或辛酰基使用具有pimeloyl- [ACP]的多肽[ACP] 合酶活性和生物化学途径，用于将己二酰[ACP]和/或己酸到的更己二酸，6-氨基己酸，6-羟基己酸，六亚甲基二胺，己内酰胺，以及1,6-己二醇中的一个。