公开(公告)号：WO2013188546A2

公开(公告)日：2013-12-19

申请号：PCT/US2013/045430

申请日：2013-06-12

Applicant: INVISTA Technologies S.à.r.l.

Inventor： PEARLMAN, Paul S. ,  CHEN, Changlin ,  BOTES, Adriana ,  CONRADIE, Alex Van Eck

IPC: C12P5/02 ,  C12N9/00 ,  C07C11/167 ,  C12N9/02

CPC classification number: C12N9/0004 ,  C12P5/026 ,  C12Y401/01033 ,  C12Y402/01127 ,  C12Y402/03027 ,  Y02E50/343

Abstract: This document describes biochemical pathways for producing butadiene by forming two vinyl groups in a butadiene synthesis substrate. These pathways described herein rely on enzymes such as mevalonate diphosphate decarboxylase, isoprene synthase, and dehydratases for the final enzymatic step.

Abstract translation: 该文献描述了通过在丁二烯合成底物中形成两个乙烯基来生产丁二烯的生化途径。 本文描述的这些途径依赖于酶，例如甲羟戊酸二磷酸脱羧酶，异戊二烯合酶和用于最终酶促步骤的脱水酶。

公开(公告)号：WO2017136344A1

公开(公告)日：2017-08-10

申请号：PCT/US2017/015842

申请日：2017-01-31

Applicant: INVISTA NORTH AMERICA S.À.R.L. ,  INVISTA TECHNOLOGIES S.À.R.L.

Inventor： BOTES, Adriana, Leonora ,  CONRADIE, Alexander, Van Eck ,  FOSTER, Alec ,  CHEN, Changlin

IPC: C12P7/04 ,  C12P7/24 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/50 ,  C12P7/64 ,  C12P13/00

CPC classification number: C12P7/40 ,  C07C47/21 ,  C07C59/147 ,  C08G69/10 ,  C08G69/26 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/80 ,  C12N9/88 ,  C12N9/90 ,  C12P7/04 ,  C12P7/24 ,  C12P7/42 ,  C12P7/46 ,  C12P7/50 ,  C12P7/6409 ,  C12P7/6427 ,  C12P13/001 ,  C12P17/182 ,  C12Y102/99006 ,  C12Y103/01031 ,  C12Y203/01032 ,  C12Y206/01018 ,  C12Y305/01 ,  C12Y402/99 ,  C12Y503/03008

Abstract: This document describes biochemical pathways for producing pimeloyl-CoA using a polypeptide having the enzymatic activity of a hydroperoxide lyase to form non-3-enal and 9- oxononanoate from 9-hydroxyperoxyoctadec-10,12-dienoate. Non-3-enal and 9-oxononanoate can be enzymatically converted to pimeloyl-CoA or a salt thereof using one or more polypeptides having the activity of a dehydrogenase , a CoA ligase , an isomerase , a reductase , a thioesterase , a monooxygenase , a hydratase , and/or a thiolase . Pimeloyl-CoA can be enzymatically converted to pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine, or 1,7-heptanediol, or corresponding salts thereof. This document also describes recombinant microorganisms producing pimeloyl-CoA, as well as pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine, and 1,7-heptanediol, or corresponding salts thereof.

Abstract translation: 该文件描述了使用具有过氧化氢裂合酶酶活性的多肽从9-氧代癸酸形成非3-烯醛和9-氧代壬酸产生庚二酰-CoA的生物化学途径， hydroxyperoxyoctadec-10,12二烯酸酯。 可使用一种或多种具有脱氢酶活性，CoA连接酶活性的多肽将非3-烯醇和9-氧代壬酸酯酶促转化为庚二酰-CoA或其盐， ，一种异构酶，一种还原酶，一种硫酯酶，一种单加氧酶，一种水合酶， ，和/或硫解酶。 庚二酰-CoA可以酶促转化为庚二酸，7-氨基庚酸，7-羟基庚酸，七亚甲基二胺或1,7-庚二醇，或其相应的盐。 该文献还描述了产生庚二酰-CoA以及庚二酸，7-氨基庚酸，7-羟基庚酸，七亚甲基二胺和1,7-庚二醇或其相应盐的重组微生物。

公开(公告)号：EP2721161A2

公开(公告)日：2014-04-23

申请号：EP12731824.4

申请日：2012-06-15

Applicant: Invista Technologies S.à.r.l.

Inventor： PEARLMAN, Paul, S. ,  CHEN, Changlin ,  BOTES, Adriana, L.

IPC: C12P7/04 ,  C12P7/42 ,  C12P7/46 ,  C12P13/02 ,  C12P13/00 ,  C12P5/02 ,  C07K14/195 ,  C07K14/37 ,  C07K14/405 ,  C07K14/415 ,  C12N1/32 ,  C12N9/10 ,  C12N9/78 ,  C12N9/88 ,  C12N9/00

CPC classification number: C12P13/005 ,  C07K14/195 ,  C07K14/37 ,  C12N1/32 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1022 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/78 ,  C12N9/88 ,  C12N15/52 ,  C12P5/02 ,  C12P5/026 ,  C12P7/04 ,  C12P7/18 ,  C12P7/42 ,  C12P7/44 ,  C12P7/46 ,  C12P13/001 ,  C12P13/02 ,  C12P17/10 ,  C12Y101/01004 ,  C12Y101/01157 ,  C12Y202/01006 ,  C12Y203/01009 ,  C12Y401/01005

Abstract: Embodiments of the invention relate to the enzymatic conversion of bioderived feedstocks to commercially valuable chemicals. The enzymatic conversions of the embodiments of the invention offer the potential for lower cost routes to these value-added chemicals. Some of the chemicals that are useful include nylon intermediates such as caprolactam, adipic acid, 1,6-hexamethylene diamine; butanediols such as 1,4-butanediol, 1,3-butanediol, and 2,3-butanediol; butanols such as 1-butanol, and 2-butanol; succinic acid, butadiene, isoprene, and 3-hydroxypropanoic acid.

Abstract translation: 本发明的实施方案涉及将生物活性原料酶催化转化成商业上有价值的化学品。 本发明实施方案的酶促转化提供了对这些增值化学品的较低成本路线的潜力。 一些有用的化学品包括尼龙中间体如己内酰胺，己二酸，1,6-六亚甲基二胺; 丁二醇如1,4-丁二醇，1,3-丁二醇和2,3-丁二醇; 丁醇如1-丁醇和2-丁醇; 琥珀酸，丁二烯，异戊二烯和3-羟基丙酸。

公开(公告)号：EP2785848A2

公开(公告)日：2014-10-08

申请号：EP12799032.3

申请日：2012-11-30

Applicant: INVISTA Technologies S.à.r.l.

Inventor： PEARLMAN, Paul S. ,  CHEN, Changlin ,  BOTES, Adriana ,  CONRADIE, Alex Van Eck

IPC: C12P5/02 ,  C12N9/00 ,  C07C11/167

CPC classification number: C12P5/026 ,  C12N9/0004 ,  C12N9/88 ,  C12N15/52 ,  C12Y401/01033 ,  C12Y402/03027 ,  Y02E50/343

Abstract: This document describes biochemical pathways for producing butadiene by forming two vinyl groups in a butadiene synthesis substrate. These pathways described herein rely on enzymes such as mevalonate diphosphate decarboxylase, isoprene synthase, and dehydratases for the final enzymatic step.

Abstract translation: 该文件描述了通过在丁二烯合成底物中形成两个乙烯基来生产丁二烯的生物化学途径。 本文所述的这些途径依赖酶如甲羟戊酸二磷酸脱羧酶，异戊二烯合酶和脱水酶用于最后的酶促步骤。

公开(公告)号：EP2938732A1

公开(公告)日：2015-11-04

申请号：EP13824254.0

申请日：2013-12-23

Applicant: Invista Technologies S.à.r.l.

Inventor： BOTES, Adriana Leonora ,  CONRADIE, Alex Van Eck ,  CHEN, Changlin ,  PEARLMAN, Paul S.

IPC: C12P7/18 ,  C12P7/42 ,  C12P7/44 ,  C12P13/00

CPC classification number: C12P7/46 ,  C12P7/18 ,  C12P7/42 ,  C12P7/44 ,  C12P13/001 ,  C12P13/005

Abstract: This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the fatty acid synthesis pathway and oxidative cleavage of long chain acyl-[acp] intermediates by a monooxgenase (e.g., cytochrome P450) such as that encoded by BioI from microorganisms such as Bacillus subtillis.

公开(公告)号：EP2861745A2

公开(公告)日：2015-04-22

申请号：EP13739305.4

申请日：2013-06-12

Applicant: Invista Technologies S.à.r.l.

Inventor： PEARLMAN, Paul S. ,  CHEN, Changlin ,  BOTES, Adriana ,  CONRADIE, Alex Van Eck

IPC: C12P5/02 ,  C12N9/00 ,  C07C11/167 ,  C12N9/02

CPC classification number: C12N9/0004 ,  C12P5/026 ,  C12Y401/01033 ,  C12Y402/01127 ,  C12Y402/03027 ,  Y02E50/343

Abstract: This document describes biochemical pathways for producing butadiene by forming two vinyl groups in a butadiene synthesis substrate. These pathways described herein rely on enzymes such as mevalonate diphosphate decarboxylase, isoprene synthase, and dehydratases for the final enzymatic step.

Abstract translation: 该文献描述了通过在丁二烯合成底物中形成两个乙烯基来生产丁二烯的生化途径。 本文描述的这些途径依赖于酶，例如甲羟戊酸二磷酸脱羧酶，异戊二烯合酶和用于最终酶促步骤的脱水酶。

公开(公告)号：EP2721164A2

公开(公告)日：2014-04-23

申请号：EP12731825.1

申请日：2012-06-15

Applicant: Invista Technologies S.à.r.l.

Inventor： PEARLMAN, Paul, S. ,  CHEN, Changlin ,  BOTES, Adriana, L.

IPC: C12P7/16 ,  C12P5/02 ,  C12P7/18

CPC classification number: C12P5/026 ,  C12N9/001 ,  C12N9/0071 ,  C12N9/88 ,  C12N15/52 ,  C12P5/02 ,  C12P7/16 ,  C12P7/18 ,  C12Y103/01035 ,  C12Y103/08 ,  C12Y114/11022 ,  C12Y401/01033 ,  C12Y402/01 ,  C12Y402/01127 ,  C12Y402/03027 ,  Y02E50/10 ,  Y02E50/343 ,  Y02P20/52

Abstract: Disclosed are methods for producing butadiene from one or more of several diverse feedstocks including bioderived feedstocks, renewable feedstocks, petrochemical feedstocks and natural gas.

Abstract translation: 该文献描述了通过在丁二烯合成底物中形成两个乙烯基来生产丁二烯的生化途径。 本文描述的这些途径依赖于酶，例如甲羟戊酸二磷酸脱羧酶，异戊二烯合酶和用于最终酶促步骤的脱水酶。

公开(公告)号：EP2726627A2

公开(公告)日：2014-05-07

申请号：EP12735729.1

申请日：2012-06-29

Applicant: Invista Technologies S.à.r.l.

Inventor： PEARLMAN, Paul, S. ,  CHEN, Changlin ,  BOTES, Adriana, L. ,  CONRADIE, Alex, Van Eck ,  HERZOG, Benjamin, D.

IPC: C12P17/10

CPC classification number: C12P7/44 ,  B29B9/16 ,  C08G69/14 ,  C08G69/16 ,  C08G69/26 ,  C08G69/28 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1025 ,  C12N9/1096 ,  C12N9/13 ,  C12N9/16 ,  C12N9/88 ,  C12N9/93 ,  C12P7/40 ,  C12P7/42 ,  C12P7/62 ,  C12P13/001 ,  C12P13/005 ,  C12P13/02 ,  C12P13/04 ,  C12P17/10 ,  C12Y102/0105 ,  C12Y102/99006 ,  C12Y208/03012 ,  C12Y208/03013 ,  C12Y301/02018 ,  C12Y301/02019 ,  C12Y301/0202 ,  C12Y602/01014 ,  C12Y602/01023

Abstract: Embodiments of the present invention relate to methods for the biosynthesis of di- or trifunctional C7 alkanes in the presence of isolated enzymes or in the presence of a recombinant host cell expressing those enzymes. The di- or trifunctional C7 alkanes are useful as intermediates in the production of nylon-7, nylon-7,x, nylon-x,7, and polyesters.

Abstract translation: 本发明的实施方案涉及在存在分离的酶或存在表达那些酶的重组宿主细胞的情况下生物合成二官能或三官能C7烷烃的方法。 二或三官能C7烷烃可用作尼龙-7，尼龙-7，x，尼龙-x，7和聚酯的生产中的中间体。