公开(公告)号：US12173348B2

公开(公告)日：2024-12-24

申请号：US16976399

申请日：2019-03-04

Applicant: Japan Science and Technology Agency

Inventor： Kazuhito Tabata ,  Yoshihiro Minagawa ,  Hiroyuki Noji

IPC: C12Q1/34 ,  C12Q1/70

Abstract: Provided is a technique that can be used to detect a pathogenic microorganism such as influenza virus with high sensitivity, and specifically, a method for detecting a reaction product, including reacting an enzyme with a substance serving as a substrate for a reaction involving the enzyme in a hydrophilic solvent that is in interfacial contact with a hydrophobic solvent, wherein the reaction product is produced directly and released from the substrate, and wherein the hydrophilic solvent contains at least one of a buffering substance or trimethylamine oxide (TMAO).

公开(公告)号：US11231370B2

公开(公告)日：2022-01-25

申请号：US16496197

申请日：2018-03-28

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Hiroyuki Noji ,  Kazuhito Tabata

IPC: G01N21/64 ,  G01N1/36 ,  G01N21/31

Abstract: Provided is a method of enclosing a microscopic body in at least some of a plurality of cavities formed in the surface of a substrate, including the step of arranging an insertion member above the cavity-formed surface of the substrate, determining relative positions of the insertion member and the substrate by a support section provided on the insertion member such that the bottom surface of the insertion member and the cavity-formed surface of the substrate face each other, thereby providing a solution introduction space between the bottom surface of the insertion member and the cavity-formed surface of the substrate, and providing a solution discharge space that is in communication with the solution introduction space, the solution discharge space being located above the bottom surface of the insertion member, and between the substrate and the insertion member, within the substrate and/or within the insertion member.

公开(公告)号：US11008603B2

公开(公告)日：2021-05-18

申请号：US16329829

申请日：2017-09-04

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Hiroyuki Noji ,  Kazuhito Tabata

IPC: C12Q1/34 ,  C12Q1/70 ,  C12Q1/04 ,  G01N33/483

Abstract: Provided is a method for detecting pathogenic microorganisms in a biological sample, which is a technique that can be used to perform high-sensitivity detection of pathogenic microorganisms, such as influenza virus, etc.