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    Microscopic body detection method and microscopic body detection device
    1.
    发明授权
    Microscopic body detection method and microscopic body detection device 有权

    公开(公告)号：US12196654B2

    公开(公告)日：2025-01-14

    申请号：US16496532

    申请日：2018-03-28

    Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

    Inventor： Hiroyuki Noji ,  Takao Ono ,  Yoshihiro Minagawa

    IPC: G01N1/36 ,  B01L3/00 ,  G01N21/64 ,  G01N21/78 ,  G01N33/569 ,  G01N33/58

    Abstract: There is provided a method of detecting a microscopic body stored in a plurality of receptacles formed separately from each other. The method, which is provided as a technique for enclosing a to-be-detected substance such as nucleic acid, protein, virus, and cell by means of a simple operation in droplets of an extremely small volume and enabling highly sensitive detection, includes the steps of (1) introducing a solvent into a space between a lower layer part in which the receptacles are formed and an upper layer part facing a surface of the lower layer part in which surface the receptacles are formed, wherein the solvent contains the microscopic body; (2) introducing gas into the space to form a droplet of the solvent in the receptacles, wherein the droplet contains the microscopic body; and (3) detecting the microscopic body present in the droplet optically, electrically, and/or magnetically.

    Kit for sealing beads and detecting target molecule
    3.
    发明授权
    Kit for sealing beads and detecting target molecule 有权

    公开(公告)号：US10267793B2

    公开(公告)日：2019-04-23

    申请号：US15351522

    申请日：2016-11-15

    Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

    Inventor： Hiroyuki Noji ,  Ryota Iino ,  Suguru Araki

    IPC: G01N31/22 ,  G01N33/543 ,  G01N21/64 ,  B01L3/00

    Abstract: This invention provides a technique enabling to detect target molecules of low concentration with high sensitivity. This invention includes (i) a step of introducing a hydrophilic solvent (42) containing beads (40),(41′) into a space (30) between (a) a lower layer section (10) including a plurality of receptacles (13) each of which is capable of storing only one of the beads (41),(41′) and which are separated from each other by a side wall (12) having a hydrophobic upper surface and (b) an upper layer section (20) facing a surface of the lower layer section (10) on which surface the plurality of receptacles (13) are provided; and (ii) a step of introducing a hydrophobic solvent (43) into the space (30), the step (ii) being carried out after the step (i).

    Method for detecting enzymatic reaction product
    4.
    发明授权
    Method for detecting enzymatic reaction product 有权

    公开(公告)号：US12173348B2

    公开(公告)日：2024-12-24

    申请号：US16976399

    申请日：2019-03-04

    Applicant: Japan Science and Technology Agency

    Inventor： Kazuhito Tabata ,  Yoshihiro Minagawa ,  Hiroyuki Noji

    IPC: C12Q1/34 ,  C12Q1/70

    Abstract: Provided is a technique that can be used to detect a pathogenic microorganism such as influenza virus with high sensitivity, and specifically, a method for detecting a reaction product, including reacting an enzyme with a substance serving as a substrate for a reaction involving the enzyme in a hydrophilic solvent that is in interfacial contact with a hydrophobic solvent, wherein the reaction product is produced directly and released from the substrate, and wherein the hydrophilic solvent contains at least one of a buffering substance or trimethylamine oxide (TMAO).

    Microscopic body enclosing method, microscopic body detection method, and microscopic body detection device
    5.
    发明授权
    Microscopic body enclosing method, microscopic body detection method, and microscopic body detection device 有权

    公开(公告)号：US11231370B2

    公开(公告)日：2022-01-25

    申请号：US16496197

    申请日：2018-03-28

    Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

    Inventor： Hiroyuki Noji ,  Kazuhito Tabata

    IPC: G01N21/64 ,  G01N1/36 ,  G01N21/31

    Abstract: Provided is a method of enclosing a microscopic body in at least some of a plurality of cavities formed in the surface of a substrate, including the step of arranging an insertion member above the cavity-formed surface of the substrate, determining relative positions of the insertion member and the substrate by a support section provided on the insertion member such that the bottom surface of the insertion member and the cavity-formed surface of the substrate face each other, thereby providing a solution introduction space between the bottom surface of the insertion member and the cavity-formed surface of the substrate, and providing a solution discharge space that is in communication with the solution introduction space, the solution discharge space being located above the bottom surface of the insertion member, and between the substrate and the insertion member, within the substrate and/or within the insertion member.

    High-density micro-chamber array and measurement method using same
    7.
    发明授权
    High-density micro-chamber array and measurement method using same 有权

    公开(公告)号：US10974246B2

    公开(公告)日：2021-04-13

    申请号：US15567431

    申请日：2016-06-07

    Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

    Inventor： Rikiya Watanabe ,  Hiroyuki Noji ,  Naoki Soga

    IPC: B01L3/00 ,  C12M1/00 ,  G01N33/48 ,  B01J19/00 ,  G01N33/487

    Abstract: A high-density micro-chamber array has a translucent flat substrate, a hydrophobic layer in which a plurality of micro-chambers are provided, and a lipid bilayer membrane formed in each of the openings of the micro-chambers, wherein an electrode is provided in each of the micro-chambers, and when the side of the substrate on which the hydrophobic layer is provided is directed upward, the micro-chamber array is configured such that with at least one of the following A) and B) being met, light entering the substrate from below is transmitted through the substrate and penetrates into the micro-chambers' interiors, and light entering the substrate from the micro-chambers' interiors is transmitted through the substrate and escapes toward below the substrate. A) The electrode is provided on an inner side surface of each of the micro-chambers. B) The electrode is transparent and provided on a bottom surface of each of the micro-chambers.

    Method for detecting target molecule
    8.
    发明授权
    Method for detecting target molecule 有权

    公开(公告)号：US10809257B2

    公开(公告)日：2020-10-20

    申请号：US16260413

    申请日：2019-01-29

    Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

    Inventor： Hiroyuki Noji ,  Ryota Iino ,  Suguru Araki

    IPC: G01N33/543 ,  G01N21/64 ,  B01L3/00

    Abstract: This invention provides a technique enabling to detect target molecules of low concentration with high sensitivity. This invention includes (i) a step of introducing a hydrophilic solvent (42) containing beads (40),(41′) into a space (30) between (a) a lower layer section (10) including a plurality of receptacles (13) each of which is capable of storing only one of the beads (41),(41′) and which are separated from each other by a side wall (12) having a hydrophobic upper surface and (b) an upper layer section (20) facing a surface of the lower layer section (10) on which surface the plurality of receptacles (13) are provided; and (ii) a step of introducing a hydrophobic solvent (43) into the space (30), the step (ii) being carried out after the step (i).

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