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    PSEUDOMONAS LIPASE
    3.
    发明专利
    PSEUDOMONAS LIPASE 未知

    公开(公告)号：CA2104838A1

    公开(公告)日：1994-02-28

    申请号：CA2104838

    申请日：1993-08-25

    Applicant: GOLDBERG STEVEN L ,  JOHNSTON ROBERT M ,  CINO PAUL M

    Inventor： GOLDBERG STEVEN L ,  JOHNSTON ROBERT M ,  CINO PAUL M

    IPC: C12N1/21 ,  C12N9/20 ,  C12N15/09 ,  C12N15/55 ,  C12P21/02 ,  C12R1/19

    Abstract: PSEUDOMONAS LIPASE Nucleic acid sequences, particularly DNA sequences, coding for all or part of a lipase of Pseudomonas sp. ATCC 21808, wherein said lipase has the amino acid sequence encoded by the nucleotide sequence of the SacI/HindIII insert of plasmid pSG312 deposited with the American Type Culture Collection, expression vectors containing the DNA sequences, host cells containing the expression vectors, and methods utilizing these materials. The invention also concerns polypeptide molecules comprising all or part of a lipase of Pseudomonas sp. ATCC 21808, wherein said lipase has the amino acid sequence encoded by the nucleotide sequence of the SacI/HindIII insert of plasmid pSG312 deposited with the American Type Culture Collection, and methods for producing these polypeptides molecules.

    PICHIA PASTORIS FORMATE DEHYDROGENASE AND USES THEREFOR
    7.
    发明申请
    PICHIA PASTORIS FORMATE DEHYDROGENASE AND USES THEREFOR 审中-公开
    PICHIA PASTORIS FORMATE DEHYDROGENASE及其用途

    公开(公告)号：WO03054155A2

    公开(公告)日：2003-07-03

    申请号：PCT/US0240235

    申请日：2002-12-16

    Applicant: SQUIBB BRISTOL MYERS CO ,  GOLDBERG STEVEN L ,  CINO PAUL M ,  PATEL RAMESH N ,  NANDURI VENKATA B ,  JOHNSTON ROBERT M

    Inventor： GOLDBERG STEVEN L ,  CINO PAUL M ,  PATEL RAMESH N ,  NANDURI VENKATA B ,  JOHNSTON ROBERT M

    IPC: C12N9/02 ,  C12P21/04 ,  C12Q1/26 ,  C12N

    CPC classification number: C12N9/0004 ,  C12Q1/26 ,  G01N2333/04

    Abstract: This invention relates to a recombinant Pichia pastoris formate dehydrogenase (FDH) enzyme that catalyzes the oxidation of formate to carbon dioxide and the simultaneous reduction of nicotinamide adenine dinucleotide (NAD+) to its reduced form (NADH). Also related are isolated nucleic acids encoding P. pastoris FDH polypeptides, and fragments and variants thereof, as well as vectors and host cells comprising these nucleic acids. Further related are isolated, recombinant P. pastoris FDH polypeptides, and fragments and variants thereof, and antibodies that specifically bind to P. pastoris FDH polypeptides, fragments, or variants. The invention also relates to methods of obtaining isolated P. pastoris FDH nucleic acids, polypeptides, and antibodies, and methods of using P. pastoris FDH in various reactions for industrial or pharmaceutical applications.

    Abstract translation: 本发明涉及催化甲酸氧化成二氧化碳并同时将烟酰胺腺嘌呤二核苷酸（NAD +）还原为还原形式（NADH）的重组巴斯德毕赤酵母甲酸脱氢酶（FDH）酶。 还涉及编码巴斯德毕赤酵母多肽的分离的核酸及其片段和变体，以及包含这些核酸的载体和宿主细胞。 进一步相关的是分离的，重组的巴斯德毕赤酵母多肽，及其片段和变体，以及特异性结合巴斯德毕赤酵母多肽，片段或变体的抗体。 本发明还涉及获得分离的巴斯德毕赤氏酵母多肽和多肽的抗体的方法，以及在工业或制药的各种反应中使用巴斯德毕赤酵母多肽的方法 应用。

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