公开(公告)号：US20170051306A1

公开(公告)日：2017-02-23

申请号：US15111084

申请日：2015-01-14

Applicant: KAGOSHIMA UNIVERSITY

Inventor： Ken-ichiro Kosai ,  Kaoru Mitsui ,  Kanako Ide

IPC: C12N15/86 ,  C12Q1/68

CPC classification number: C12N15/86 ,  A61K48/00 ,  C07K2319/92 ,  C12N2740/15043 ,  C12N2830/008 ,  C12Q1/6897 ,  G01N33/5011 ,  G01N33/5073

Abstract: The invention provides a method to search for a best promoter for reliably targeting a cancerization-causing cell in human ES/iPS cells. Discovering a gene which can kill or remove a cancerization-causing cell contained in human ES/iPS cells with high efficiency is also provided. A method is provided for removing an undifferentiated cell, which remains after the differentiation of human ES/iPS cells into desired cells, with high efficiency and comprehensively. A viral vector is provided which includes a nucleotide sequence and a recombination cassette. The nucleotide sequence contains a target gene and a killing gene that are linked to each other through a sequence that enables the simultaneous expression of the two genes by one promoter. The recombination cassette contains a promoter region which is so linked as to enable the expression of the labeling gene and the killing gene. The viral vector may contain a promoter specific to an undifferentiated cell.

Abstract translation: 本发明提供了一种搜索用于可靠地靶向人ES / iPS细胞中的致癌细胞的最佳启动子的方法。 还提供了能够以高效率杀死或除去人ES / iPS细胞中所含致癌细胞的基因的基因。 提供了一种用于去除未分化细胞的方法，该细胞在人ES / iPS细胞分化成所需细胞后保持高效率和全面。 提供了包含核苷酸序列和重组盒的病毒载体。 核苷酸序列包含通过能够通过一个启动子同时表达两个基因的序列彼此连接的靶基因和杀死基因。 重组盒含有如此连接的启动子区，以使得能够表达标记基因和杀死基因。 病毒载体可以含有对未分化细胞特异性的启动子。