公开(公告)号：EP2165758A4

公开(公告)日：2016-08-03

申请号：EP08764904

申请日：2008-05-30

Applicant: KANEKA CORP

Inventor： KAWAI YOSHIKAZU ,  KAWAHARA NAOMI

IPC: B01J20/24 ,  A61M1/18 ,  C07K1/22 ,  C07K17/02 ,  G01N30/88

CPC classification number: C07K1/16 ,  B01D15/00 ,  B01J20/289 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3242 ,  B01J20/3274 ,  C07K1/22

Abstract: The present invention relates to a porous base matrix having formyl group, which base matrix has a structure represented by the formula (2) as a spacer, which structure is obtained by cleaving a group represented by the formula (1): wherein, R 1 represents a group forming a five-membered ring or a six-membered ring with -CH (OH) -CH (OH) -; a method for producing the porous base matrix; and an adsorbent obtained by binding a ligand on the porous base matrix. By the present invention, a high-intensity base matrix for an adsorbent and an adsorbent are provided. The amount of a ligand leaked from the adsorbend is small.

公开(公告)号：EP2383035A4

公开(公告)日：2017-11-22

申请号：EP09830207

申请日：2009-12-03

Applicant: KANEKA CORP ,  JNC CORP

Inventor： KAWAI YOSHIKAZU ,  KAWASAKI HIROAKI ,  KAWAHARA NAOMI

IPC: B01J20/24 ,  B01D15/08 ,  B01J20/32 ,  G01N30/88

CPC classification number: C08B37/00 ,  B01J20/289 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3229 ,  B01J20/3244 ,  B01J20/3248

Abstract: The present invention relates to a method for producing a formyl group-containing porous base matrix, comprising the steps of introducing a spacer in a formyl group-containing porous particle; and then oxidizing the spacer with periodic acid and/or a periodate, to transform the part of the spacer into a formyl group; wherein the formyl group content in the porous particle after introduction of the spacer is not more than 3 µmol per 1 mL of the porous particle. Also, the present invention relates to a method for producing an adsorbent, comprising the step of immobilizing an amino group-containing ligand on the formyl group-containing porous base matrix. According to the present invention, a formyl-group containing porous base matrix and an adsorbent produced from the porous base matrix of which adsorption amount is high and which is has high strength and of which ligand is difficult to be leaked are provided.

公开(公告)号：JP2011252929A

公开(公告)日：2011-12-15

申请号：JP2011193297

申请日：2011-09-05

Applicant: Kaneka Corp ,  株式会社カネカ

Inventor： KAWAI YOSHIKAZU ,  KAWAHARA NAOMI

IPC: G01N30/88 ,  B01J20/24

Abstract: PROBLEM TO BE SOLVED: To conveniently and inexpensively provide a porous carrier which has strength capable of purifying an antibody and the like without problems on a large scale and under a high linear velocity and has a large amount of adsorption of a substance to be purified; an adsorbent for purification using the same; a manufacturing method thereof; and a purifying method using the same.SOLUTION: The present invention provides: a porous carrier having the compressive stress of 0.01 MPa to 1 MPa at the time of 5% compression, the compressive stress of 0.03 MPa to 3 MPa at the time of 10% compression, and the compressive stress of 0.06 MPa to 5 MPa at the time of 15% compression; the adsorbent for purification using the same; the manufacturing method thereof; and the purification method using the same. As a preferred embodiment, the porous carrier can have larger strength by acting a long chain cross-linking agent having a functionality of 2 or higher. The present invention makes it possible to purify an antibody and the like on a large scale, at a high speed, and a low cost.

Abstract translation: 要解决的问题：为了方便和廉价地提供具有能够大规模和高线性速度纯化抗体等的强度的多孔载体，并且具有大量的物质吸附 被净化 用其进行纯化的吸附剂; 其制造方法; 和使用其的纯化方法。 解决方案：本发明提供：压缩5％时的压缩应力为0.01MPa〜1MPa，压缩10％时的压缩应力为0.03MPa〜3MPa的多孔载体， 15％压缩时的压应力为0.06MPa〜5MPa; 用于其的净化吸附剂; 其制造方法; 和使用其的纯化方法。 作为优选的实施方案，多孔载体可通过作用具有2或更高官能度的长链交联剂而具有较大的强度。 本发明使得可以大规模，高速和低成本地纯化抗体等。 版权所有（C）2012，JPO＆INPIT

公开(公告)号：JP2014194425A

公开(公告)日：2014-10-09

申请号：JP2014103780

申请日：2014-05-19

Applicant: Kaneka Corp ,  株式会社カネカ ,  Jnc Corp ,  Jnc株式会社

Inventor： KAWAI YOSHIKAZU ,  KAWAHARA NAOMI

IPC: G01N30/88 ,  B01D15/08 ,  B01J20/24 ,  B01J20/30 ,  C07K1/22 ,  G01N30/60

Abstract: PROBLEM TO BE SOLVED: To provide easily and at low cost a porous carrier which has a strength enough to purify an antibody or the like in large scale and under a high linear velocity and of which the adsorption amount of a purification target object is large, an adsorbent for purification using the same, a manufacturing method of the same and a purification method using the same.SOLUTION: A porous carrier, an adsorbent for purification using the same, a manufacturing method of the same and a purification method using the same are disclosed. Regarding the porous carrier, a compression stress under compression of 5% is 0.01 MPa or more and 1 MPa or less, a compression stress under compression of 10% is 0.03 MPa or more and 3 MPa or less and a compression stress under compression of 15% is 0.06 MPa or more and 5 MPa or less. A strength of the porous carrier can be increased by action of two or more functionalities of a long-chain crosslinking agent. An antibody or the like can be purified in large scale, at high speed and at low cost.

Abstract translation: 要解决的问题：为了容易且低成本地提供一种多孔载体，该多孔载体具有足够的强度，可以大规模和高线速度纯化抗体等，其净化对象物的吸附量大， 使用其的纯化用吸附剂及其制造方法和使用该吸附剂的纯化方法。溶液：多孔载体，使用该吸附剂的纯化，其制造方法和使用该吸附剂的纯化方法 披露。 关于多孔载体，压缩5％时的压缩应力为0.01MPa以上1MPa以下，压缩10％时的压缩应力为0.03MPa以上且3MPa以下，压缩时的压缩应力为15 ％为0.06MPa以上且5MPa以下。 可以通过长链交联剂的两种或多种官能团的作用来增加多孔载体的强度。 抗体等可以大规模，高速且低成本地纯化。

公开(公告)号：JP2008279366A

公开(公告)日：2008-11-20

申请号：JP2007125820

申请日：2007-05-10

Applicant: Kaneka Corp ,  株式会社カネカ

Inventor： KAWAI YOSHIKAZU ,  KAWAHARA NAOMI

IPC: B01J20/24 ,  B01D15/08 ,  G01N30/88

Abstract: PROBLEM TO BE SOLVED: To provide a porous carrier which has strength to be capable of conducting the purification of antibody and the like with large scale and under high wire-speed without problems and which has a large amount of adsorption of the substance to be purified; to provide an adsorbent for purification using the same; to provide a manufacturing method thereof; and to simply and inexpensively provide the purification process using the same.
SOLUTION: The porous carrier is characterized in that: the compression stress at 5% compression is from 0.01 MPa or more to 1 MPa or less; the compression stress at 10% compression is from 0.03 MPa or more to 3 MPa or less; and the compression stress at 15% compression is from 0.06 MPa or more to 5 MPa or less. Also the adsorbent using the same, the manufacturing method thereof and the purification method using the same are provided. As a preferable embodiment, the strength of the porous carrier can be enlarged by causing a long-chain cross-linker with two or more functions to act. Thus, the purification of the antibody and the like can be performed with a large scale under a high speed and in a low cost.
COPYRIGHT: (C)2009,JPO&INPIT

Abstract translation: 待解决的问题：提供一种具有能够大规模，高线速地进行抗体等的纯化的强度的多孔载体，没有问题，并且具有大量的物质吸附 待净化 以提供使用其的纯化吸附剂; 提供其制造方法; 并且简单且廉价地提供使用其的纯化方法。 解决方案：多孔载体的特征在于：5％压缩时的压缩应力为0.01MPa以上至1MPa以下; 10％压缩时的压缩应力为0.03MPa以上3MPa以下。 15％压缩时的压缩应力为0.06MPa以上5MPa以下。 还提供了使用其的吸附剂，其制备方法和使用其的纯化方法。 作为优选实施方案，通过使具有两个或更多个功能的长链交联剂起作用，可以扩大多孔载体的强度。 因此，能够以高速，低成本地进行大规模的抗体等的纯化。 版权所有（C）2009，JPO＆INPIT