公开(公告)号：GB2210058B

公开(公告)日：1991-07-31

申请号：GB8821954

申请日：1988-09-19

Applicant: KAO CORP

Inventor： UEDA SHINTA ,  SUGIMURA YUKIO ,  OTSUJI KAZUYA ,  HIGASHI WAYAYOSHI ,  OKAMOTO KIKUHIKO

IPC: A01G1/00 ,  C12N5/04 ,  A01H4/00 ,  C12N5/00 ,  C12N5/02

Abstract: A method is provided for the regeneration of an individual coconut plantlet. It comprises culturing a tissue explant of an undifferentiated male flower of a coconut tree in a culture medium, which contains 10-50 ppm of an auxin, 0.1-3% of a polysaccharide produced by a bacterium of Pseudomonas and 0.05-1% of activated carbon, in a dark place until a shoot-like structure appears; and then culturing under exposure to light of 3,000-20,000 luxes while repeating its subculture in a fresh culture medium.

公开(公告)号：GB2210058A

公开(公告)日：1989-06-01

申请号：GB8821954

申请日：1988-09-19

Applicant: KAO CORP

Inventor： UEDA SHINTA ,  SUGIMURA YUKIO ,  OTSUJI KAZUYA ,  HIGASHI WAYAYOSHI ,  OKAMOTO KIKUHIKO

IPC: A01G1/00 ,  C12N5/04 ,  A01H4/00 ,  C12N5/00 ,  C12N5/02

Abstract: A method is provided for the regeneration of an individual coconut plantlet. It comprises culturing a tissue explant of an undifferentiated male flower of a coconut tree in a culture medium, which contains 10-50 ppm of an auxin, 0.1-3% of a polysaccharide produced by a bacterium of Pseudomonas and 0.05-1% of activated carbon, in a dark place until a shoot-like structure appears; and then culturing under exposure to light of 3,000-20,000 luxes while repeating its subculture in a fresh culture medium.