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    PROCESS FOR PRODUCING SACCHARIDE
    3.
    发明专利
    PROCESS FOR PRODUCING SACCHARIDE 未知

    公开(公告)号：CA2710504A1

    公开(公告)日：2009-07-09

    申请号：CA2710504

    申请日：2008-12-16

    Applicant: KAO CORP

    Inventor： NOJIRI NAOKI ,  UMEHARA MASAHIRO ,  TOMIOKA KEIICHIRO ,  KAWANO TAKAKO ,  KITSUKI TOMOHITO ,  OKUTSU MUNEHISA ,  OGAWA AKINORI

    IPC: C12P19/00 ,  C12P7/06 ,  C12P7/56

    Abstract: A process for producing saccharide, including saccharifying decrystallized cellulose prepared from a raw material containing cellulose having cellulose I-type crystallinity of more than 33%, the process including: treating the cellulose-containing raw material by means of a mill to reduce the cellulose I-type crystallinity of the cellulose to 33% or less, wherein the cellulose- containing raw material has a cellulose content of a residue obtained by removing water from the cellulose-containing raw material of 20% by weight or more, to thereby prepare decrystallized cellulose, and causing a cellulase and/or a hemicellulase to act on the decrystallized cellulose.

    Procedimiento de producción de sacárido
    6.
    发明专利
    Procedimiento de producción de sacárido 未知

    公开(公告)号：ES2393164T3

    公开(公告)日：2012-12-19

    申请号：ES08868615

    申请日：2008-12-16

    Applicant: KAO CORP

    Inventor： NOJIRI NAOKI ,  UMEHARA MASAHIRO ,  TOMIOKA KEIICHIRO ,  KAWANO TAKAKO ,  KITSUKI TOMOHITO ,  OKUTSU MUNEHISA ,  OGAWA AKINORI

    IPC: C12P19/00 ,  C12P7/06 ,  C12P7/56

    Abstract: Un procedimiento de producción de sacárido, que incluye la sacarificación de la celulosa descristalizadapreparada a partir de una materia prima que contiene celulosa que tiene una cristalinidad de la celulosa de tipo Isuperior al 33% según se calcula a partir de la siguiente fórmula (1):Cristalinidad de la Celulosa de Tipo I (%) >=[(I22.6 - I18.5)/I22.6] x 100 (1)en la que I22,6 es la intensidad de la difracción de un plano reticular (plano 002), como se mide con un ángulo dedifracción 2θ de 22,6° por análisis de difracción de Rayos X, y I18,5 es la intensidad de difracción de un resto amorfocomo se mide con un ángulo de difracción 2θ de 18,5º por análisis de difracción de Rayos X, procedimiento quecomprende:tratamiento de la materia prima que contiene celulosa por medio de un molino para reducir la cristalinidadde la celulosa de tipo I de la celulosa a un 10% o inferior, en el que la materia prima que contiene celulosatiene un contenido de celulosa de un residuo obtenido por eliminación del agua de la materia prima quecontiene celulosa en un 20% en peso o superior, para preparar de ese modo celulosa descristalizada, yprovocando que una celulasa y/o hemicelulasa actúe sobre la celulosa cristalizada, para de ese modosacarificar la celulosa descristalizada,en el que la materia prima que contiene celulosa tiene una densidad aparente de 100 a 500 kg/m3 y untamaño medio de partícula de 0,01 a 1 mm.

    Alkali protease
    7.
    发明专利
    Alkali protease 审中-公开

    公开(公告)号：JP2004201532A

    公开(公告)日：2004-07-22

    申请号：JP2002372506

    申请日：2002-12-24

    Applicant: Kao Corp ,  花王株式会社

    Inventor： OKUDA MITSUYOSHI ,  SUMITOMO NOBUYUKI ,  OGAWA AKINORI ,  KOBAYASHI TORU

    IPC: C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/54

    Abstract: PROBLEM TO BE SOLVED: To provide a new alkali protease which is used for detergents, has a larger mol. wt. and a lower amino acid sequence homology than those of conventional alkali protease for detergents or than those of conventional subtilisin, and to provide a gene encoding the same. SOLUTION: This protein is (a) a protein originated from a Bacillus sp. and comprising a specific amino acid sequence or (b) a protein comprising (a) the protein whose one or more amino acids are deleted, replaced or added and having an alkali protease activity. The protein is a protein comprising an amino acid sequence having the homology of ≥80% based on the amino acid sequence of (a) the protein and an alkali protease activity. A gene encodes the protein. A recombinant vector contains the gene. A transformant has the recombinant vector. COPYRIGHT: (C)2004,JPO&NCIPI

    PECTINIC ACID LIASE GENE
    8.
    发明专利
    PECTINIC ACID LIASE GENE 失效

    公开(公告)号：JP2000125877A

    公开(公告)日：2000-05-09

    申请号：JP30069298

    申请日：1998-10-22

    Applicant: KAO CORP

    Inventor： SAWADA KAZUHISA ,  OGAWA AKINORI ,  SUMITOMO NOBUYUKI ,  HATADA YUJI ,  KOBAYASHI TORU

    IPC: C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/88 ,  C12N15/00 ,  C12R1/125 ,  D06M16/00

    Abstract: PROBLEM TO BE SOLVED: To obtain a new pectinic acid liase gene encoding a specific amino acid sequence, capable of decomposing polysaccharides constituting plant cell wall and useful for producing the above enzyme helpful for wastewater treatment, decomposition and removal of plant cell wall, cotton fiber scouring or the like, or the like. SOLUTION: The objective gene is a new pectinic acid liase gene encoding an amino acid sequence of the formula and capable of including alterations selected from a group consisting of at least one amino acid deletion, substitution, and enables us to produce pectinic acid liase in an isolated state on a large scale which is used for aftertreatment of wastewater including pectin, decomposition and removal of plant cell wall, cotton fiber scouring and a detergent ingredient and is used as a detergent, food processing agent, fiber treating agent or the like. The gene is obtained by preparing genomic DNA from Bacillus sp. KSM-P15 strain (FERM BP-6241) and by amplifying it as a template through PCR using its partial sequences as primers.

    Polygalacturonase
    9.
    发明专利
    Polygalacturonase 有权
    多聚半乳糖

    公开(公告)号：JP2012019757A

    公开(公告)日：2012-02-02

    申请号：JP2010161751

    申请日：2010-07-16

    Applicant: Kao Corp ,  花王株式会社

    Inventor： OGAWA AKINORI ,  NAGAMI ATSUSHI ,  SAEKI KATSUHISA

    IPC: C12N9/24 ,  C12N1/20 ,  C12P19/00

    CPC classification number: Y02P20/52

    Abstract: PROBLEM TO BE SOLVED: To provide a polygalacturonase exhibiting an endo type decomposition style, acting in a neutral to an alkaline region and stable to surfactants and chelating agents, a method for producing the same and a method for producing a mono- and oligo-galacturonic acid by using the enzyme.SOLUTION: This polygalacturonase includes the following zymologic properties: (1) the activity of acting on a polygalacturonic acid, hydrolyzing the α-1,4 bond of the polygalacturonic acid in an endo type, and producing the oligo-galacturonic acid; (2) the optimal reaction pH being in the vicinity of pH 7 (in tris-hydrochloric acid buffer); (3) the optimal reaction temperature being approximately 40 to 50°C (in the tris-hydrochloric acid buffer, at pH 7.0); and the (4) molecular weight being approximately 52,000 (by a gel filtration method).

    Abstract translation: 待解决的问题：提供一种表现出内分泌型分解型的聚半乳糖醛酸酶，其在中性至碱性区域中作用并对表面活性剂和螯合剂具有稳定性，其制备方法及其制备方法 寡聚半乳糖醛酸。 该多聚半乳糖醛酸酶包括以下酶学性质：（1）作用于多聚半乳糖醛酸的活性，水解内切型聚半乳糖醛酸的α-1,4键，并产生寡聚半乳糖醛酸; （2）最佳反应pH在pH7附近（在三盐酸缓冲液中）; （3）最佳反应温度为约40〜50℃（在三盐酸缓冲液中，pH7.0）; （4）分子量约为52,000（通过凝胶过滤法）。 版权所有（C）2012，JPO＆INPIT

    Method for producing sugar
    10.
    发明专利
    Method for producing sugar 有权
    生产糖的方法

    公开(公告)号：JP2009171952A

    公开(公告)日：2009-08-06

    申请号：JP2008209154

    申请日：2008-08-15

    Applicant: Kao Corp ,  花王株式会社

    Inventor： NOJIRI NAOKI ,  UMEHARA MASAHIRO ,  KIZUKI TOMOHITO ,  OKUTSU TAKAHISA ,  OGAWA AKINORI ,  KAWANO TAKAKO

    IPC: C12P19/14

    CPC classification number: Y02E50/17

    Abstract: PROBLEM TO BE SOLVED: To provide a method for producing a sugar, which comprises subjecting an amorphous cellulose prepared by lowering a cellulose I type crystallinity from a cellulose-containing raw material, as a substrate, to an enzymatic reaction using a cellulase or the like, and by which the sugar can efficiently be produced in excellent productivity. SOLUTION: Provided is the method for producing the sugar, characterized by treating a cellulose-containing raw material having a bulk density of 100 to 500 kg/m 3 and an average particle size of 0.01 to 1 mm with a grinder to prepare amorphous cellulose having a decreased cellulose I type crystallinity of ≤33%, and then saccharifying the amorphous cellulose with a cellulase and/or a hemicellulase. The cellulose I type crystallinity (%)=[(I 22.6 -I 18.5 )/I 22.6 ]×100 wherein, I 22.6 is a diffracted intensity of a lattice plane (angle of diffraction 2θ= 22.6°) in X-ray diffraction; and I 18.5 is a diffracted intensity of an amorphous portion (angle of diffraction 2θ= 18.5°). COPYRIGHT: (C)2009,JPO&INPIT

    Abstract translation: 待解决的问题：提供一种糖的制造方法，其包括使用纤维素酶将来自含纤维素原料的纤维素I型结晶度降低的非晶质纤维素作为底物进行酶反应 可以以优异的生产率有效地生产糖。 解决方案：提供糖的制造方法，其特征在于，将堆积密度为100〜500kg / m 3，SP 3的平均粒径为0.01〜 1mm的研磨机，制备纤维素I型结晶度降低≤33％的无定形纤维素，然后用纤维素酶和/或半纤维素酶糖化无定形纤维素。 纤维素I型结晶度（％）= [（I SB = 22.6）/ I SB> 22.6 ]×100其中，I 22.6 是X射线衍射中的晶格面（衍射角2θ= 22.6°）的衍射强度。 并且I 18.5 是非晶部分的衍射强度（衍射角2θ= 18.5°）。 版权所有（C）2009，JPO＆INPIT

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